| Movie 1 |
To delineate the defect 10 min post lesion, a network of the tight junction
protein occludin (red) as well as the cells’ nuclei (stained by DAPI,
blue) are shown in three dimensions using confocal fluorescence microscopy.
The movie shows x–y scans parallel to the epithelium (quadratic image),
x–z scans at the indicated green line (rectangular image on top of
the quadratic image), and y–z scans at the indicated red line (rectangular
image to the right of the quadratic image). The blue line in the x–z
and y–z scans indicates the x–y plane shown.
|
| Movie 2–4 |
To visualize the funnel-like defect in space, we generated a
three-dimensional reconstruction by superposition of optical sections showing
the tight junction proteins. The movies show the 3-D reconstruction with
ZO-1 (green) and occludin (red) merged (yellow). Six minutes post lesion
(Movie 2), the ring of tight junction proteins was hardly visible at the
bottom of the defect. Ten minutes post lesion (Movie 3) the ring encloses
a visible gap, which 15 min post lesion (Movie 4) appears to be closed.
Note also the tight junctions between intact cells. |
| Download: |
Movie 1 (3.3Mb) MPEG Movie
Movie 2 (3.8Mb) MPEG Movie
Movie 3 (3.8Mb) MPEG Movie
Movie 4 (4.7Mb) MPEG Movie |
