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a SavicBrain Research Institute, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland
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Abstract |
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-amino-3hydroxy-5methyl-4-isoxadepropionate (AMPA)-type glutamate receptors in CA3–CA1 synapses. This form of synaptic strengthening occludes long-term potentiation (LTP) and enhances long-term depression (LTD), processes involved in learning and memory. These changes in synaptic transmission and plasticity, which are fully blocked with N-methyl-D-aspartate (NMDA) receptor antagonists, may underlie epilepsy induction and seizure-associated memory deficits.
(Received 4 August 2003;
accepted after revision 27 October 2003;
first published online 31 October 2003)
Corresponding author M. H. Abegg: Brain Research Institute, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland. Email: mhabegg{at}access.unizh.ch
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Introduction |
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TopAbstractIntroductionMethodsResultsDiscussionReferences |
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Methods |
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TopAbstractIntroductionMethodsResultsDiscussionReferences |
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Organotypic hippocampal slice cultures were prepared from 6-day-old Wistar rat pups killed by decapitation (Gähwiler et al. 1997), following a protocol approved by the Veterinary Department of the Canton of Zurich. After 2 weeks in vitro, slices were randomly allocated into three groups that were incubated for 15 ± 3 h (overnight) either in serum-based medium alone or in medium containing BMC (50 µM), or BMC plus the specific NMDA receptor antagonist (R,E)-4-(3-phosphonoprop-2-enyl)piperazine-2-carboxylic acid (CPP 40 µM; donated by Novartis, Basel, Switzerland).
Electrophysiology
Field recordings of spontaneous bursting activity were performed at 35°C (incubation temperature) with patch pipettes (3–5 M
) containing 2 M NaCl in culture medium. Whole-cell voltage-clamp recordings of excitatory synaptic currents were obtained using an Axopatch 200B amplifier (Axon Instruments, Foster City, CA, USA) and pipettes containing (mM): 140 potassium or caesium gluconate, 10 KCl, 5 Hepes, 1.1 EGTA, 4 MgCl2, 10 phosphocreatine, pH 7.3, 285 mosmol l-1, unless otherwise stated. Slices were perfused with warmed (32°C) saline containing (mM): 137 NaCl, 2.7 KCl, 2.8 CaCl2 2 MgCl2, 11.6 NaHCO3, 0.4 NaH2PO4, 5.6 glucose and phenol red (10 mg l-1), pH 7.4, unless otherwise mentioned. Only cells with a series resistance between 10 and 15 M were included.
Miniature excitatory postsynaptic currents (mEPSCs) were recorded at –70 mV in the presence of 0.5 µM tetrodotoxin (TTX, Latoxan, Valence, France), 50 µM picrotoxin, 50 µM BMC and 40 µM CPP using potassium gluconate-based intracellular solution (see above). Miniature inhibitory postsynaptic currents (mIPSCs) were recorded at –70 mV in the presence of 0.5 µM TTX, 20 µM 2,3-dioxo-6-nitro-1,2,3,4-tetrahydrobenzo(f)quinoxaline-7-sulphonamide (NBQX) and 40 µM CPP using the following intracellular solution (mM): 140 caesium gluconate, 10 NaCl, 1 MgCl, 10 Hepes, 0.4 Mg2GTP, 0.1 EGTA, pH 7.3, 285 mosmol l-1. mEPSCs and mIPSCs were analysed offline with the ‘Mini Analysis Program’ (Synaptosoft, Leonia, CA, USA) using a detection threshold of 5 pA. Cumulative histograms were constructed by pooling 300 consecutive events from each cell. Average traces were obtained for each experiment by aligning individual mEPSCs to their rising phases; fitting a single exponential on the 90–10% of the decaying signal yielded the decay time constant.
Excitatory postsynaptic currents (EPSCs) were evoked using a monopolar glass stimulation electrode filled with extracellular medium and placed into stratum radiatum close to the recording electrode, in the presence of 50 µM picrotoxin, 50 µM BMC, 4 mM Mg2+ and 4 mM Ca2+, and after a cut between CA3 and CA1. AMPA receptor-mediated EPSCs were evoked at –70 mV, whereas NMDA receptor-mediated EPSCs were evoked at +40 mV during blockade of AMPA receptors by application of 20 µM NBQX.
Field excitatory postsynaptic potentials (fEPSPs) were recorded in the stratum radiatum of the CA1 region with a glass electrode containing 2 M NaCl and 3 mM BMC after cutting between areas CA3 and CA1. Stimuli (0.1 ms) were delivered to CA3 axons at 0.25 Hz by a bipolar glass electrode filled with extracellular solution. LTP was induced by theta burst stimulation composed of five trains at 5 Hz, each consisting of five stimuli delivered at 100 Hz. LTD was induced by stimulating at 3 Hz for 5 min (Dudek & Bear, 1992). The levels of potentiation and depression were estimated for each cell by measuring the average slope of field potentials in the middle third of its rising phase over 5 min taken 25 min after the end of LTP or LTD induction and were expressed as a percentage of baseline slope.
Average values are expressed as means ±S.E.M. Statistical comparisons were made using the Student's t tests. Drugs were from either Tocris Cookson (Bristol, UK) or Sigma (Buchs, Switzerland), unless otherwise specified.
Preparation and application of Semliki Forest virus vectors
The cDNA for green fluorescent protein (GFP)-tagged GluR1 (Mack et al. 2001) was inserted into the XhoI and AvrII sites of the pSFV2gen vector, yielding pSFV2gen-GFP-GluR1. Viral particles were produced according to our standardized procedure (Ehrengruber, 2002). Slices were injected with diluted (1 : 50) virus stock over two or three sites in the CA1 pyramidal cell layer, returned to the incubator, and cultured for an additional 2 days.
Confocal microscopy and image analysis
Optical stacks (27.2 µm x 27.2 µm, step size 0.5 µm, 8-bit, 512 x 512 pixels) were acquired on a Leica SP2 confocal microscope (Leica Microsystems, Heidelberg, Germany) equipped with a heated (32°C) perfusion chamber and a HCX APO x 63 water immersion objective lens (NA = 0.9). No averaging was employed, laser power, gain and offset of the photomultipliers were equal for corresponding images. Spines were identified in images obtained in control and BMC containing medium. Relative spine fluorescence was determined by averaging pixel values usually over two layers containing spines and also from the equivalent places before treatment. Mean values of spine fluorescence measured after overnight incubation (after) were normalized to mean values before incubation (before) both in control and BMC-treated slices. Background fluorescence in nearby regions without any obvious structures was subtracted from all measurements. Images shown are maximal intensity projections of several optical sections, median filtered with pixel width 1. All analysis was performed with ImageJ (National Institutes of Health, USA).
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Results |
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Recordings of field potentials in the CA1 stratum radiatum under culture conditions (perfusion of culture medium at 35°C) revealed that acute application of BMC induced epileptiform bursts (burst frequency: 0.05 ± 0.007 Hz, burst duration: 4.2 ± 0.6 s, n= 17, Fig. 1A) that were never observed in control conditions (n= 17, Fig. 1B) and correspond to synchronous population activity of neurones within the culture (Traub & Wong, 1982). Epileptiform bursts lasted longer than 500 ms and were thus classified as ictal activity (see Karnup & Stelzer, 2001). Simultaneous recordings of field potentials in the CA3 region (presynaptic activity) and the membrane potential of a CA1 pyramidal cell (postsynaptic activity) revealed that during bursting pre- and postsynaptic cells are activated concomitantly (n= 3, Fig. 1C). Bursting was still present after overnight incubation in BMC-containing medium, suggesting that slices have an ability to burst during the entire period of disinhibition (n= 13); in addition, burst frequency was significantly higher after overnight incubation than after acute BMC application (burst frequency: 0.13 ± 0.032 Hz, n= 13, P= 0.02), suggesting that prolonged disinhibition accelerates bursting.
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Epileptiform activity enhances AMPA receptor-mediated synaptic transmission
To examine whether epileptiform activity affects excitatory synaptic transmission, we recorded AMPA receptor-mediated mEPSCs from CA1 pyramidal cells that had burst overnight. We found a significant increase in both the frequency (control: 1.06 ± 0.2 Hz, n= 10, BMC: 2.56 ± 0.3 Hz, n= 7, 241 ± 28% of control, P= 0.002, Fig. 3) and the amplitude (control: 20.4 ± 1.2 pA, n= 10; BMC: 26 ± 1.3 pA, n= 7, 127 ± 6.3% of control, P= 0.021, Fig. 3) of mEPSCs in BMC-treated slices. No changes in the 10–90% rise time (control: 1.15 ± 0.05 ms, n= 10, BMC: 1.15 ± 0.13 ms, n= 7, 100 ± 11% of control, P= 0.99), the decay time constant (control: 3.78 ± 0.11 ms; BMC: 3.46 ± 0.41 ms, 92 ± 10% of control, P= 0.4), the input resistance (control: 138 ± 6 M, BMC: 143 ± 7 M, 104 ± 5% of control, P= 0.57) and the membrane capacitance (control: 110 ±8 pF, BMC: 104 ± 5 pF, 95 ± 5% of control, P= 0.61) were found after overnight bursting. The increase in mEPSC frequency may be due to a higher probability of vesicular glutamate release or alternatively to an increased number of functional synapses. To test the first possibility, we measured the amplitude ratio of evoked AMPA receptor-mediated EPSCs in response to paired-pulse stimulation of CA3 axons at different time intervals (50, 75, 100, 150 and 200 ms). A change in this ratio reflects a change in the probability of vesicular release (Debanne et al. 1996). There were no significant differences in the paired-pulse ratios of evoked AMPA receptor-mediated EPSCs at any of the intervals tested between control and BMC-treated slices (P > 0.26 at all intervals, n= 5 in each group, Figs 4C and D), suggesting no change in the probability of vesicular release.
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Next, we tested whether the enhancement of synaptic transmission shows NMDA receptor dependence, by incubating slices overnight in medium containing BMC + CPP. Although this treatment did not have a major effect on BMC-induced bursting (see Fig. 1F), it prevented the rise in mEPSC frequency (1.12 ± 0.27 Hz, n= 8, 105.6 ± 25.4% of control, P= 0.86, Fig. 3) and amplitude (21.4 ± 1.3 pA, n= 8, 104.9 ± 6.3% of control, P= 0.41, Fig. 3), as well as the increase of the AMPA-to-NMDA current ratio (1.3 ± 0.4, n= 5, 65 ± 20% of control, P= 0.15, Fig. 4A and B), without affecting the kinetics of mEPSCs (10–90% rise time: BMC + CPP: 1.03 ± 0.06 ms, n= 8, 90 ± 5% of control, P= 0.2; decay time constant: BMC + CPP: 3.63 ± 0.20 ms, 95 ± 5% of control, P= 0.5). Taken together, these data show that epileptiform activity leads to an NMDA receptor-dependent activation of silent synapses and to potentiation of functional synapses.
Epileptiform activity promotes delivery of GluR1-GFP to dendritic spines
To directly monitor the redistribution of AMPA receptors following epileptiform activity, we overexpressed recombinant green fluorescent protein-tagged GluR1 AMPA receptor subunits (GluR1-GFP) (Mack et al. 2001) in CA1 pyramidal cells using a Semliki Forest virus expression system (Ehrengruber, 2002) (Fig. 5A). These recombinant subunits can form homomeric receptors that are translocated into synapses upon tetanic stimulation but that remain largely excluded from synapses under normal synaptic transmission (Shi et al. 2001). Two days post transfection, we collected confocal images of GFP fluorescence from primary and secondary apical dendrites before and after overnight incubation with BMC. Bursting led to a significant increase in fluorescence in dendritic spines (143 ± 21.6% of before, P= 0.0002 paired t test, n= 26 spines in n= 5 slices, Figs 5B and C), whereas the amount of fluorescence at the corresponding location in images obtained before bursting was often near the background level. The mean spine fluorescence did not change in control slices (97 ± 14% of before, P= 0.62 paired t test, n= 15 spines in n= 8 slices, Figs 5B and C). Often we found that bursting activity induced formation of fluorescence clusters in dendrites, which we did not further quantify. Given that most excitatory synapses of CA1 pyramidal cells are localized on dendritic spines, the translocation of GluR1-GFP into spines suggests that bursting activity drives AMPA receptors into synapses.
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An NMDA receptor-dependent increase in synaptic transmission, as we observe after bursting, is known to occur during LTP in area CA1 (Collingridge et al. 1983). Moreover, Hebbian conditions for LTP induction are established during bursting, i.e. pre- and postsynaptic cells are activated simultaneously (see Fig. 1C). It is therefore conceivable that bursting-induced synaptic strengthening and LTP share the same expression mechanism. If this is the case, then LTP induced with a conventional stimulation protocol should be reduced or even occluded after overnight bursting. To test this possibility, we compared the degree of potentiation in control, BMC- and BMC + CPP-treated slices after theta burst stimulation of CA3 axons. Stable LTP lasting at least 30 min was induced in control slices (142 ± 15% of baseline fEPSP slope, n= 11, P= 0.011, Fig. 6A and C) whereas no LTP could be elicited in BMC-treated slices (83 ± 9% of baseline fEPSP slope, n= 10, P= 0.08, Fig. 6A and C). When NMDA receptors were blocked during overnight epileptiform activity, thereby preventing synaptic strengthening, LTP of similar magnitude as in control slices was induced after washout of CPP (163 ± 22% of baseline fEPSP slope, n= 11, P= 0.01, Fig. 6A and C). These results show that synapses that were strengthened by overnight bursting cannot be further potentiated, indicating that epileptiform activity and LTP have a common expression mechanism.
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The occlusion of LTP and the enhancement of LTD are consistent with the hypothesis that the bursting-induced strengthening of CA3–CA1 synapses corresponds to LTP.
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Discussion |
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TopAbstractIntroductionMethodsResultsDiscussionReferences |
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Epileptiform activity caused a significant increase in the frequency and amplitude of AMPA receptor-mediated mEPSCs. mEPSC frequency varies with the number of functional synapses on a given cell and with the probability of vesicular release, whereas the number and/or conductance of receptors within a synapse define the amplitude. Prolonged bursting could increase mEPSC frequency by increasing the probability of vesicular release. However, our data are not consistent with this hypothesis as the presynaptic parameter paired-pulse ratio was unchanged after overnight bursting, and the increase in mEPSC frequency was blocked by NMDA receptor antagonists despite a maintained high level of neuronal activity. Our data suggest, rather, that bursting-induced activation of silent synapses accounted for the increase in mEPSC frequency. In support of this, we observed an increase in the evoked AMPA-to-NMDA current ratio after bursting. This ratio represents the relative AMPA and NMDA receptor content at both functional and silent synapses. The more than 5-fold increase in the AMPA-to-NMDA current ratio was accompanied by a 30% increase in mEPSC amplitude, suggesting that potentiation of functional synapses only partially accounted for the observed increase in the AMPA-to-NMDA current ratio and implying a major contribution from silent synapse activation. Taken together, these results show that bursting increases the number of functional AMPA receptors in both functional and silent synapses. Alternatively, a decrease in NMDA receptor-mediated synaptic transmission may contribute to a bursting-induced increase in AMPA-to-NMDA current ratio. Although we did not directly investigate NMDA receptor function, the changes in mEPSCs, GluR1-GFP trafficking, and LTP/LTD levels clearly identify AMPA receptor-mediated synaptic transmission as a target of epileptiform activity. Moreover, the ability to induce LTD, which is NMDA receptor-dependent at the CA3–CA1 synapse (Debanne et al. 1994), indicates that NMDA receptors remain functional after bursting.
That bursting enhances excitatory synaptic transmission is supported by our finding that disinhibition led to spine delivery of GluR1-GFP. In line with this, it has been recently shown that LTP induction relocalizes alphavirally overexpressed recombinant GluR1 to the synapse (Hayashi et al. 2000). Hence, we presume that bursting also leads to the insertion of GluR1-GFP in synapses which are localized on dendritic spines. As reported by Shi et al., we also observed dendritic clusters of GluR1-GFP after epileptiform activity, the meaning of which is obscure and has been suggested to be related to the spine apparatus (Shi et al. 1999).
After overnight epileptiform activity, no LTP could be induced with theta burst stimulation, indicating that synapses were already maximally potentiated, i.e. bursting raised the level of baseline synaptic efficacy to its maximal state. Since the amounts of bi-directional plastic changes depend on the level of baseline synaptic efficacy (Rioult-Pedotti et al. 2000; Savic et al. 2003), it was not surprising that occlusion of LTP was accompanied by a concomitant increase of LTD in slices that had shown bursting activity overnight. Thus bursting and tetanic stimulation both activate the same pathway resulting in potentiation of excitatory synapses. This view is additionally supported by our finding that bursting, like LTP, increases synaptic efficacy through activation of NMDA receptors (see Figs 1, 3 and 6).
The strengthening of excitatory synapses during bursting potentially affects all excitatory synapses that have the capacity for NMDA receptor-dependent plasticity. This strengthening will reinforce the excitatory network and may shift the balance of excitation and inhibition towards excitation, thereby increasing the bursting probability (Bains et al. 1999; Staley et al. 2001). We suggest that bursting-induced reinforcement of the excitatory network accounts for the sustained spontaneous bursting that we observed after BMC washout. In line with this, when synaptic strengthening was prevented by NMDA receptor blockade during bursting, no spontaneous epileptiform activity was observed after washout of BMC and CPP. We speculate that reinforcement of the excitatory network represents a final common pathway on to which all epilepsy models that use high neuronal activity as an induction stimulus converge. This shift may also explain how seizure foci can spread from one brain region to another, as occurs during secondary epileptogenesis (Mayersdorf & Schmidt, 1982).
Several factors including changes in inhibitory synaptic transmission, alterations in membrane transporters and synaptic reorganization may participate in the induction and maintenance of seizure activity. Our data are not consistent with a change in inhibition (see Fig. 2), but rather emphasize the role of strengthening excitatory synaptic connections through an LTP-like mechanism, as postulated previously (Ben-Ari & Represa, 1990). Our findings are consistent with results from kindled rats which display progressively increasing EPSPs in the course of kindling (Sutula & Steward, 1986) and with reports showing that bursting activity potentiates excitatory synapses (Buzsaki et al. 1987; Schneiderman et al. 1994; Bains et al. 1999) and that NMDA receptor antagonists can prevent (Ben-Ari & Gho, 1988; Croucher et al. 1988; Stasheff et al. 1989) or delay epileptogenesis (Sato et al. 1988; Durmuller et al. 1994; DeLorenzo et al. 1998).
As LTP is thought to be involved in memory acquisition (see, e.g. Tsien et al. 1996), bursting-induced LTP occlusion may explain the transient decrease in learning ability observed after seizures (Cain et al. 1993; McNamara et al. 1993; Rutten et al. 2002). Accordingly, we speculate that the recovery of memory formation after cessation of seizure activity (Cain et al. 1993; Rutten et al. 2002) mirrors the time course of LTP decay (Villarreal et al. 2002).
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