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Departments of 1 Physiology2 Veterinary Biomedical Sciences3 the Dalton Cardiovascular Research Center, University of Missouri, Columbia, MO, USA
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(Received 2 February 2004;
accepted after revision 23 March 2004;
first published online 26 March 2004)
Corresponding author J. T. Cunningham: Department of Pharmacology, UTHSCSA, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA. Email: cunninghamt{at}uthscsa.edu
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Introduction |
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The hindlimb-unloaded (HU) rat is a model of microgravity and bed rest (Morey-Holton & Globus, 2002). In this rat model, the hindlimbs are elevated above the floor of the cage such that the weight of the animal is borne by the forelimbs. Blood volume is shifted to the head and thoracic cavity (Tipton et al. 1996). Diuresis and natriuresis follow and a reduction in plasma volume occurs (Deavers et al. 1980; Musachia et al. 1992; Martel et al. 1996). Attenuation of water intake (Steffen et al. 1984) and baroreflex control of sympathetic nerve activity (Moffitt et al. 1998) have also been reported. In addition to the cephalic pooling of body fluid, hypokinesia may also contribute to the diuresis and natriuresis associated with HU (Bouzeghrane et al. 1996; Zorbas et al. 1996).
In these studies we examined the behavioural component of body fluid regulation by measuring water ingestion and voluntary sodium intake during a brief (24 h) period of HU. Since the diuresis and natriuresis associated with HU occurs during the first 24 h (Deavers et al. 1980; Musachia et al. 1992; Martel et al. 1996), we chose to focus the study on this time period in order to more clearly determine how these early responses to HU would influence water and sodium intake. Based on Kaufman's observations (Kaufman, 1984; Toth et al. 1987) and previous studies that have demonstrated that HU produces cephalic pooling of body fluids followed by diuresis and natriuresis (Deavers et al. 1980; Tucker et al. 1987) it was hypothesized that both water intake and intake of sodium chloride solutions would be diminished during acute HU, contributing to the decrease in plasma volume. After observing that 24 h HU was associated with an increase in sodium intake, we tested the specifity of the sodium appetite by giving HU rats access to a potassium chloride solution and examined the effects of sodium availability on changes in plasma volume during 24 h HU. We also tested the roles of aldosterone and the renin–angiotensin system in the sodium appetite produced by 24 h HU by measuring plasma aldosterone and plasma renin activity during HU and by administering an aldosterone antagonist, spironolactone during 24 h HU.
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Methods |
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In order to address the role of restraint stress in this paradigm, the rats were all subjected to a training protocol in which they were HU for 1 h over 3 days. To accomplish HU, a harness was attached to the base of the tail and hooked to a swivel on a nylon line above the cage such that the animal was at an approximately 45 deg angle (Moffitt et al. 1998). The animal could move freely about the cage using its front paws.
Surgical procedures
Catheter implantation. Forty-five rats were instrumented with femoral venous and carotid arterial catheters for plasma volume measurements. Each rat was anaesthetized with pentobarbital sodium (50 mg kg–1I.P.) and the skin around the incision site was shaved and cleaned. Using aseptic technique, an incision was made and a blunt dissection performed to isolate the vessels from the surrounding tissue. The vessel was tied proximally and clamped. A small incision was made and a catheter was introduced into the vessel, advanced into the abdominal aorta or vena cava and secured in place with suture. Each catheter was constructed of PE 10 heat-melded to PE 50 tubing. The catheters were tunnelled subcutaneously and exteriorized between the scapulae. Each catheter was filled with heparinized saline and plugged with a 23 g obturator. An additional group of rats was implanted with only carotid artery catheters for withdrawing blood for plasma renin measurements. Each rat was anaesthetized with pentobarbital sodium (50 mg kg–1I.P.) and prepared as described above. Once the carotid artery was isolated and clamped a small incision was made, a Silastic-tipped PE 50 catheter was inserted into the vessel and advanced just into the descending aorta. These catheters were exteriorized between the scapulae. Each rat was allowed to recover for at least 3–5 days prior to being used in another protocol.
Osmotic minipump implantation. Thirty-seven rats were implanted with osmotic mini-pumps (Alzet model 2004, 0.25 µl h–1; Cupertino, CA, USA) for either intracerebroventricular (I.C.V.) or subcutaneous (S.C.) infusions. Osmotic mini-pumps were filled with either spironolactone (Sigma Chemical Co., St Louis, MO, USA) dissolved in artificial cerebrospinal fluid (400 µg ml–1) containing 1% alcohol or only the alcohol-containing vehicle (Francis et al. 2001). The pumps were incubated for at least 48 h in a 37°C water bath and weighed prior to implantation. Surgeries were performed using aseptic technique and the rats were anaesthetized with pentobarbital sodium (50 mg kg–1I.P.). For I.C.V. infusions, the pumps were connected to a piece of 23 gauge stainless steel tubing (Small Parts Miami Lakes, FL, USA), which was bent at a 90 deg angle, with polyethylene tubing. The free end of the 23 gauge tubing was placed in the left lateral ventricle using stereotaxic technique. A small hole was drilled in the skull at 1.0 mm posterior and 1.5 mm lateral to bregma (using the level skull technique, Paxinos & Watson, 1997). The cannula was put into the hole so that it extended 6 mm down into the brain from the surface of the skull and then it was cemented into place with dental acrylic and jeweller's screws. The osmotic pumps were then sutured into a small pocket made under the skin at the base of the neck. For S.C. infusions, the pumps were prepared and placed under the skin at the base of the neck just as they were implanted for the I.C.V. infusions.
Sodium and water intake
Rats were trained to the HU procedure as described above. Rats were randomly divided into five different groups. Distilled water, and rat chow was available to all the animals ad libitum. One group was given only distilled water to drink (n= 10). In a second group of rats 0.9% sodium chloride was available in addition to water (n= 11). Water intake and sodium chloride solution intake were monitored daily. Each cage was outfitted with calibrated tubes containing either water or a salt solution so that fluid intake could be measured to the nearest 0.1 ml. In addition calibrated centrifuge tubes were hung below the tubes containing distilled water or salt solution to collect any fluid that was lost due to leakage or spilling. Water and saline solution that was recovered in these tubes was subtracted from the total measured. Total fluid intake was determined by adding the amounts of water and saline solution that were ingested. Measurements were made at the same time each day.
At least 2 days following training, rats were hindlimb unloaded for 24 h. HU was accomplished in the same manner as training. Water and sodium chloride solution intakes were monitored over the 24 h. Rats were returned to normal posture following 24 h HU and water and sodium chloride solution intakes were monitored during a 24 h recovery period.
Because 0.9% NaCl may be palatable to the rats, we examined NaCl intake using a solution that is less palatable. To accomplish this, the identical testing protocol was repeated in a third group of rats using a 1.8% NaCl solution (n= 7). In order to test the specificity of the effects of HU on sodium intake, a fourth group was trained and suspended (HU) as previously described but had access to a 1.8% KCl and water rather than a NaCl solution (n= 6). Another group of rats was trained for HU and given 1.8% KCl and water to drink but was not suspended (n= 4).
Plasma volume measurements
Rats were trained for the HU protocol as above with 0.9% sodium chloride solution available in addition to distilled water. For this study the rats were instrumented with chronic indwelling catheters. The sodium chloride solution was withdrawn and rats were implanted with a carotid artery and femoral vein catheter. After 3 days recovery (during which the sodium chloride solution was not available (Lane et al. 1997), the 0.9% sodium chloride solution was returned to half the rats. The remainder of the animals had only water available, and served as controls.Half of the control group and half of the rats with 0.9% sodium chloride were then suspended for 24 h. Thus, there were four experimental groups: control posture, water only (n= 11); HU, water only (n= 11); control posture, 0.9% sodium chloride and water available (n= 11); and HU, 0.9% sodium chloride and water available (n= 12). At the end of this 24 h period, plasma volumes were measured using the Evans blue dye method as previously described (Campbell et al. 1958; Farjanel et al. 1997). Briefly, 25 µg of dye in 100 µl saline was injected via the femoral venous catheter. The catheter was flushed with 200 µl saline. After 5 min the arterial catheter was cleared of saline and a blood sample was drawn into a heparinized syringe. The HU rats remained suspended until the blood samples were drawn. The blood was centrifuged and plasma sample obtained. The dye content of the sample was determined spectrophotometrically at 610 nm and compared to a standard curve constructed using known amounts of Evans blue dye and plasma from donor rats. Plasma volume per 100 g body weight was calculated for statistical analysis.
Plasma renin activity and aldosterone assays. Rats were trained for HU as previously described. One group of the rats was suspended for 24 h (n= 9) while another group served as controls (n= 7). Both groups were given only water to drink since the ingestion of a sodium-containing solution may influence aldosterone and renin release. At the end of the 24 h period each rat was anaesthetized with pentobarbital sodium (50 mg kg–1I.P.) and immediately decapitated. A 2–4 ml volume of whole blood from each rat was collected in tubes containing EGTA and centrifuged for 10 min at 4 g in a refrigerated centrifuge. The plasma from each sample was removed and separated into two different samples. Plasma renin activity (PRA) was determined using a RIANEN angiotensin I 125I radioimmunoassay kit (DuPont NEN, Boston, MA, USA) designed to measure PRA by the quantification of generated angiotensin I. The assay sensitivity (defined as the concentration of hormone at 95%B/Bo) was 6.0 pg and the intra- and intercoefficients of variation averaged 8% and 10%, respectively. Serum (or plasma) aldosterone was measured using a Coat-A-Count solid-phase 125I radioimmunoassay kit from Diagnostic Products Corporation (Los Angeles, CA, USA). The assay sensitivity was 9 pg ml–1 and the intra- and intercoefficients of variation averaged 5.6% and 12.8%, respectively. All assays were performed at the RIA core at the University of Iowa. Control experiments indicate that the PRA levels obtained by anaesthesia and decapitation are not significantly different from samples obtained from plasma collected from unanaesthetized rats using catheters placed in the carotid artery (decapitation (n= 7) 4.19 ± 1.7 ng ml–1 h–1 angiotensin I versus catheter (n= 10) 3.18 ± 1.6 ng ml–1 h–1 angiotensin I, P > 0.05).
Spironolactone experiments
Based on the results of the study on plasma aldosterone, we tested the role of aldosterone on the salt appetite generated during 24 h HU by blocking aldosterone receptors with the antagonist spironolactone (Francis et al. 2001). Rats were randomly assigned to five groups: controls (n= 8); I.C.V. infused with vehicle (n= 8); I.C.V. infused with spironolactone (400 µg ml–1; n= 9); S.C. infused with vehicle (n= 7); S.C. infused with spironolactone (400 µg ml–1; n= 13). The four groups receiving I.C.V. or S.C. infusions were implanted with osmotic minipumps as described above. The fifth group did not receive osmotic minipumps and served as unoperated controls.
Each rat was individually housed in the Plexiglas cages for HU 3–4 days prior to receiving the minipumps and baseline water and 1.8% NaCl intakes were recorded. After receiving the minipumps the rats were allowed 7 days to recover from surgery and daily water and 1.8% NaCl intakes were recorded. During this period the rats were trained for HU after which they were suspended for 24 h as described above. Body weight was also measured daily during these experiments. At the end of the 24 h HU, the rats were deeply anaesthetized with pentobarbital sodium (100 mg kg–1I.P.) and the osmotic minipumps were removed and weighed to ensure that they worked properly. In addition, the rats with I.C.V. cannulae implants were given injections of Evans blue dye (2% w/v) through the cannulae to verify that the cannulae terminated in the lateral ventricle.
Statistical analysis
Data were analysed by ANOVA. When appropriate, multiple comparisons of significant results were made using Student-Newman-Keuls tests. The level of significance was set at P < 0.05. The relationship between PRA and plasma aldosterone in HU and control posture rats was determined using a Pearson product moment correlation coefficient. In situations when data were not normally distributed or variance was non-homogeneous (spironolactone experiment) a natural log transformation was used. Statistical analyses were performed using commercially available software (SigmaStat, v. 2.03, Jandel Scientific). All data are presented as means ±S.E.M.
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Results |
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The water intake of the experimental groups is illustrated in Fig. 1. Rats offered only distilled water to drink exhibited a decrease in water intake during the 24 h of HU. Intakes returned to baseline levels during the 24 h recovery period following HU.
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Examination of the total fluid volume ingested revealed that only rats allowed access to distilled water as their sole source of fluid decreased total fluid intake (Fig. 3). When sodium chloride solutions were available in addition to distilled water, fluid intake (ml water + ml sodium chloride solution) was not different from control during the 24 h HU or 24 h recovery period.
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Plasma volume
HU resulted in a significant decrease in plasma volume in animals having distilled water alone available to drink Fig. 4). Plasma volume was reduced approximately 13% by the end of 24 h HU compared to the control group. There was no significant difference in plasma volume between animals in the control posture with or without 0.9% sodium chloride available. In contrast to animals with access to water alone, HU did not result in decreased plasma volume in rats that had also 0.9% sodium chloride available during HU. Consistent with the plasma volume measurements, rats in the control posture with water or with water and sodium chloride solution available, and HU rats with sodium chloride available showed little change in weight during the 24 h period (0.4 ± 1.9 g, –1.4 ± 1.4 g and 0.6 ± 2.6 g, respectively). However, HU rats with only water available to drink had a significant decrease in weight (–4.8 ± 2.1 g) during the 24 h of HU.
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HU was associated with a significant increase in plasma aldosterone compared to the control posture rats (Fig. 5, P < 0.05). HU did not significantly affect PRA (Fig. 5, P > 0.05). In the control posture rats, plasma aldosterone was positively correlated with PRA (r2= 0.693). However, aldosterone was not positively correlated with PRA in the HU rats (r2=–0.37).
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There were no significant differences in any group between pre-surgical and post-surgical baseline daily water or 1.8% NaCl intakes. During 24 h HU, water intake was significantly decreased across all of the groups (Table 1). Thus, spironolactone administered either I.C.V. or S.C. did not significantly affect the decrease in water intake associated with HU.
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Discussion |
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HU and water intake
In the present studies, water intake was decreased during 24 h HU although this decrease was not statistically significant in every group. Water intake is not commonly measured during HU and the results of such studies have been variable. Decreases in water intake have been observed in HU rats and mice (Deavers et al. 1980; Steffen et al. 1984). However, other investigators using the rat model have observed a non-significant trend toward a decrease in water intake (McCombs et al. 1996) or no reduction in water intake during HU (Martel et al. 1996). Bouzeghrane et al. (1996) did not observe changes in water intake in either HU rats or tethered non-suspended controls. Important differences exist between these investigations and the present study. Variation in the angle of HU might contribute to the differences in the results as could the age or weights of the animals (Morey-Holton & Globus, 2002).
Hypokinesia has also been shown to influence water intake and body fluid balance (Zorbas et al. 1996). In rats subjected to 90 days of hypokinesia water intake significantly decreased while fluid and electrolyte excretion significantly increased (Zorbas et al. 1996). The decrease in water intake during hypokinesia is also associated with decreased food intake (Zorbas et al. 1996). Some investigators have observed a decrease in food intake during HU (Steffen et al. 1984; McCombs et al. 1996) believed to be associated with a reduced energy expenditure that accompanies the limited use of the hindlimbs (Cintron et al. 1990; Stein & Gaprindashvili, 1994). Thus, hypokinesia could contribute to the decrease in water intake related to a decrease in energy expenditure as well as produce the diruesis and natriuresis that is associated with HU although urine output was not measured in the present study. Nevertheless, this hypothesis seems unlikely given the fact that the rats are clearly able to drink, and they maintain their fluid intake when they are given NaCl solutions.
The changes in drinking behaviour observed in HU rats in this study are consistent with the changes observed in humans during bed rest (Grigor'ev & Egorov, 1992) and exposure to microgravity (Grigor'ev et al. 1979; Grigor'ev & Egorov, 1992; Smith et al. 1997). Common to both space flight and bed rest is a reduction in water intake (Grigor'ev et al. 1992; Smith et al. 1997). This change in behaviour contributes to the reduction of body fluid volume.
HU and NaCl intake
Contrary to our initial hypothesis that water and sodium intake both would be suppressed by HU, intake of NaCl-containing solutions increased during 24 h HU. This increased intake was not observed when the rats were offered a KCl-containing solution with water. This suggests that the increase was specific for NaCl. Total fluid intake was significantly reduced from baseline by 24 h HU when the rats had access only to water. When the rats also had access to a NaCl-containing solution, their total fluid intake during 24 h HU was not different from baseline. These results indicate that 24 h HU is associated with a significant increase in the consumption of NaCl-containing solutions that prevents a significant decrease in total fluid intake during 24 h HU. Toth et al. (1987) demonstrated that stretch of the aortocaval junction attenuated sodium intake induced by volume depletion with a hyperoncotic colloid and to treatment with deoxycorticosterone acetate. Since it has been reported that in this model rats experience an increase in central venous pressure during HU (Musachia et al. 1992; Martel et al. 1996), we hypothesized that similar mechanisms might be activated that would suppress NaCl ingestion. However, we observed a marked increase in intake of sodium chloride solutions. It could be that a natriuresis associated with restraint stress or hypokinesia (Bouzeghrane et al. 1996; Zorbas et al. 1996) stimulated the increased sodium consumption. Hypokinesia-induced diuresis and natriuresis may have prevented significant cephalic pooling of body fluids and prevented the activation of cardiac afferents that would have suppressed the ingestion of the NaCl-containing solutions.
The possibility that the sodium appetite is produced by stress seems unlikely because previous studies have shown that in the rat restraint stress either significantly reduces (Bensi et al. 1997) or does not affect sodium appetite (Howell et al. 1999). Furthermore, studies have shown that some forms of stress produce a decrease in sodium excretion (Koepke & Dibona, 1985; Veelken et al. 1996). Thus the increase in drinking of NaCl solutions associated with 24 h HU is not likely to be a stress response.
We conducted additional experiments in an attempt to determine the mechanism responsible for the increased sodium intake associated with 24 h HU. Aldosterone is thought to contribute to the production of sodium appetite with the renin–angiotensin system (Sakai et al. 1986; Epstein, 1990) and circulating angiotensin has been demonstrated to be important in stimulating sodium appetite (Thunhorst & Fitts, 1994). In the current study, PRA and plasma aldosterone were measured in rats after 24 h of HU with only water to drink. Aldosterone was significantly increased in the HU animals while we failed to find a significant change in PRA. This result is consistent with previous studies measuring plasma renin activity in HU rats that show that plasma renin activity is reduced during chronic HU (Tucker et al. 1987; McCombs et al. 1996). Aldosterone has been found to be elevated during HU in one study (McCombs et al. 1996). Perhaps the natriuresis observed during HU results in hyponatraemia that promotes aldosterone production.
Based on our measurements of aldosterone, we hypothesized that increased plasma aldosterone may contribute to the increased sodium intake that we observed during HU. To test this hypothesis we administered an aldosterone antagonist centrally and peripherally during HU. This protocol was selected based on the results of a previous study that demonstrated selective actions of spironolactone in the central nervous system (Francis et al. 2001). The results show that central aldosterone receptor blockade with spironolactone significantly decreased sodium intake during 24 h HU. These results suggest that the sodium appetite produced by 24 h HU is mediated by centrally acting aldosterone. However, aldosterone appears to be acting independently of the peripheral renin–angiotensin system.
Adrenocorticotrophic hormone (ACTH) also promotes the release of aldosterone. ACTH release might be expected due to the stress of HU and in fact ACTH has been demonstrated to be elevated early in HU (Thomason & Booth, 1990). Additionally, ACTH has been demonstrated to produce a sodium appetite following several days of administration (Blaine et al. 1975; Weisinger et al. 1978; Blair-West et al. 1989). The long time course usually required for the production of sodium appetite in this model would make ACTH an unlikely candidate. The mechanism that produced the increase in plasma aldosterone associated with 24 h HU remains to be determined.
A decrease in plasma volume of approximately 13% was apparent in HU rats that had only distilled water available to drink. This is consistent with previous literature (Martel et al. 1996). Presumably, the increase in central venous pressure results in a withdrawal of renal sympathetic nerve activity and decrease in vasopressin secretion (Kaczmarczyk et al. 1983; DiBona & Kopp, 1997; Grindstaff et al. 2000) thus promoting the excretion of sodium and water. Reduction in water intake would also contribute to the reduction in body fluid volume. Kaufman (1990) has also suggested that atrial stretch leads to atrial natriuretic factor release that promotes shunting of fluid from the vascular space into the lymphatic system to reduce vascular volume. Additionally, hypokinesia may also contribute to the decrease in plasma volume (Zorbas et al. 1996).
In this experiment, HU rats ingesting a sodium solution maintained plasma volume. Thus, it is possible that rats given NaCl solutions to drink during HU may be less susceptible to orthostatic intolerance if reduced plasma volume is one of its major contributing factors (Convertino, 1996). Previous studies suggest that increased salt intake or salt loading can reduce orthostatic intolerance in humans following space flight (Bungo et al. 1985) and improve orthostatic tolerance in patients with orthostatic-related syncope (El Sayed & Hainsworth, 1996; Mtinangi & Hainsworth, 1998). A recent study by Bayorh et al. 2000) demonstrated that placing rats on a high sodium diet significantly affected the changes in baroreflex function produced by 7 days of HU. Their data show that HU rats on a 8% NaCl diet failed to show a rightward shift in the baroreflex control of heart rate (Bayorh et al. 2000). Thus salt loading may be an effective countermeasure for some of the effects of cardiovascular deconditioning.
The HU rat has emerged as one of the major animal models used for land-based studies of the possible effects of microgravity associated with space flight (Morey-Holton & Globus, 2002). Similar to microgravity and bed rest, HU is reported to produce a shift of body fluids to the head and thorax resulting in increases in central venous pressure (Martel et al. 1996; McCombs et al. 1996). This increase in central blood volume is sensed by cardiac atrial baroreceptors which mediate the diuresis and natriuresis observed during the early phases of HU (Deavers et al. 1980; Tucker et al. 1987; Martel et al. 1996; McCombs et al. 1996). The resulting reduction in plasma volume contributes to the return of central venous pressure to normal levels between 12 and 24 h later (Martel et al. 1996). This reduction in plasma volume is believed to contribute to the development of cardiovascular deconditioning observed following HU (Martel et al. 1996). In addition to cephalic shifts in body fluid, hypokinesia may also contribute to changes in body fluid balance produced by HU. Bouzeghrane et al. (1996) showed that many of the changes in body fluid balance that are associated with HU may be the results of stress or hypokinesia related to connecting the rats to the suspension system without changing their posture. These findings may challenge the validity of the HU rat model since the cephalic fluid shift that is normally observed in humans (Grigor'ev et al. 1979; Greenleaf, 1984; Harper & Lyles, 1988; Leach et al. 1996) may not be the primary cause of the changes in body fluid homeostasis associated with HU.
The control of body fluid balance is complex, requiring the central integration of neural and humoral inputs related to volume, pressure and osmolality. We had expected to find that the HU rat exhibited coordinated behavioural responses that were consistent with the reduction of body fluid volume, namely decreased water and sodium intake. Rather, we observed that 24 h HU is associated with an aldosterone-dependent increase in sodium intake despite the fact that water intake is reduced. This increased sodium intake produced a normal total fluid intake that apparently kept the HU rats from reducing their plasma volume. It could be that opposing systems, those contributing to reduction of body fluid through diuresis, natriuresis, and the reduction of water intake and those promoting the maintenance of body fluid through ingestion of sodium, may be activated simultaneously. Alternatively, the sodium appetite could be the result of the diuresis and natriuresis although water intake remains suppressed. Whether these changes in body fluid balance are related to cephalic pooling associated with suspension during HU or produced by hypokinesia remains to be determined. Future experiments will examine changes in water and sodium intake in chronic HU rats since it is a more clinically relevant model for cardiovascular deconditioning and determine whether or not sodium loading is an effective countermeasure for the chronic effects of cardiovascular deconditioning.
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