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Pflanzenphysiologie, Zentrum für Molekularbiologie der Pflanzen (ZMBP), Universität Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany
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3 mM. Electric current measurements and intracellular pHi determinations suggest that RhBG acts as an electroneutral NH4+–H+ exchanger.
(Received 5 May 2004;
accepted after revision 26 July 2004;
first published online 29 July 2004)
Corresponding author U. Ludewig: Pflanzenphysiologie, Zentrum für Molekularbiologie der Pflanzen (ZMBP), Universität Tübingen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany. Email: uwe.ludewig{at}zmbp.uni-tuebingen.de
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Introduction |
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98.6%) is in the charged form. Both species can be transported across plasma membranes, with NH3 being relatively permeable via lipid diffusion, although its permeability varies according to the lipid and protein composition (Lande et al. 1995). Some plasma membranes do not show significant NH3 fluxes (Kikeri et al. 1989; Priver et al. 1993). The term ammonium will be used for NH4+/NH3 throughout the manuscript, while the chemical symbols will be used to denote the charged NH4+ or uncharged NH3 species specifically. The same formalism applies to methylammonium. The charged species, NH4+, requires transport proteins for membrane passage. AMT/MEP-type proteins encode NH4+ transporters and these proteins were initially molecularly identified from yeast and plants (Marini et al. 1994; Ninnemann et al. 1994). The sequence similarity of rhesus polypeptides from erythrocytes (Rh) to AMT/MEP-type proteins suggested that the Rh-type proteins also encode NH4+ transporters (Marini et al. 1997). Indeed, ammonium transport by RhAG glycoprotein was reported after heterologous expression in yeast (Marini et al. 2000). However, RhAG and a homologue, RhCG (Liu et al. 2000), were functionally different from yeast and plant NH4+ transporters (Marini et al. 2000). Functional differences between AMT/MEP-type and Rh-type proteins were also found after heterologous expression in oocytes, where RhAG function seems best explained as an NH4+–H+ antiport (Westhoff et al. 2002), while RhCG was suggested to be involved in NH4+ and NH3 transport (Bakouh et al. 2004). Xenopus laevis oocytes provide an ingenious system for analysing most solute and ion transporters, but need to be used with care for analysis of ammonium transporters since they express endogenous NH4+-permeable ion channels that are activated by NH3 influx and intracellular alkalinization (Burckhardt & Fromter, 1992; Cougnon et al. 1996; Burckhardt & Burckhardt, 1997; Boldt et al. 2003).
Rhesus B glycoprotein (RhBG), another member of the mammalian NH4+ transporter family with a molecular weight of the non-glycosylated protein of 40 kDa, is expressed in key organs associated with ammonium transport and metabolism (Liu et al. 2001; Quentin et al. 2003). Besides expression in ovary and skin, RhBG glycoprotein is detected in the liver, the gatekeeper organ of nitrogen metabolism and nitrogen balance in animals. Animals generally excrete surplus nitrogen via the urea cycle but nitrogen can also be excreted as ammonium. This process is performed by the kidney, another site of RhBG expression, and is used to control the blood plasma pH.
Ammonium metabolism in kidney functions to dispose of H+ in urine. Ammonium is initially produced from glutamine catabolism in the proximal tubule, where it is released into the tubular fluid (Knepper et al. 1989). It is then concentrated in the inner medulla, and is filtered from the tubular fluid in the ascending limb of the loop of Henle, where NH4+ replaces potassium at several transporters at the potassium-binding site; examples include the Na+–K+-ATPase, Na+–K+–2Cl– cotransporter, and potassium channels. RhBG and RhCG glycoproteins are expressed in the distal nephron and collecting duct, where ammonium is finally released in a pH-dependent manner into the urine. RhBG expression is detected in the connecting tubule (CNT), the initial collecting tubule (ICT), the cortical collecting duct (CCD), the outer medullary collecting duct (OMCD) and the inner medullary collecting duct (IMCD) (Quentin et al. 2003; Verlander et al. 2003). Interestingly, RhBG protein is predominantly identified at basolateral membranes, while the homologous RhCG glycoprotein, which is mostly expressed in the same cells, is strictly located in apical membranes (Eladari et al. 2002; Quentin et al. 2003; Verlander et al. 2003). The distal renal epithelia are thought to be much less permeable to NH4+, but they mediate net NH3 transport (Knepper et al. 1989; Flessner et al. 1991). Net NH3 transport may be either by direct NH3 diffusion or by NH4+–H+ exchange. The major form of acid secretion in the urine is as ammonium, so that each H+ is exchanged for NH4+, which is finally excreted.
In this study, currents were recorded by two-electrode voltage-clamp from RhBG-expressing oocytes. In addition, uptake experiments with the radiolabelled ammonium analogue methylammonium and intracellular pHi measurements were performed and identifed RhBG as an NH4+–H+ exchanger. We additionally found that NH4+–H+ exchange is associated with small ammonium-dependent and -independent currents in Xenopus oocytes.
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Human RhBG was cloned from a kidney cDNA library (Clontech) and checked by sequencing. The plasmid pOO2 (Ludewig et al. 2002) containing the coding region of human RhBG plus a 9 bp sequence (Kozak motif: GCCGCCACC) upstream the ATG ligated into the EcoRI site was kindly provided by J.-P.-Cartron and C. Lopez (INSERIM, Paris, France). Capped cRNA was transcribed by SP6 RNA polymerase in vitro using mMessage mMachine (Ambion), after linearizing the plasmid with MluI.
Preparation and injection of oocytes
Ovarian lobes were removed from adult female Xenopus frogs anaesthetized by immersion in 0.2% tricaine methanesulphonate (Sigma) for 45–60 min. Frogs were humanely killed after the final oocyte collection. Animal care and experiments followed approved institutional guidelines at the University of Tübingen. Oocytes (Dumont stage V or VI) were defolliculated using 10 mg ml–1 collagenase (Boehringer, Germany) and 5 mg ml–1 trypsin inhibitor (Sigma, Germany) for 1–2 h and injected with 15–50 nl of cRNA ( 15–50 ng per oocyte). Oocytes were kept after injection for 3–4 days at 16°C in ND96 solution (mM: 96 NaCl, 2 KCl, 1.8 CaCl2, 1 MgCl2, 5 Hepes (pH 7.4)), supplemented with 2.5 mM sodium pyruvate and gentamycin (20 µg ml–1).
Electrophysiological measurements
Standard bath solutions contained (mM): 100 NaCl, 2 CaCl2, 2 MgCl2, 4 Tris, pH adjusted to 7.5 with Mes. For measurements at higher pH, the standard solution adjusted to pH 8.5 with Tris was used. For measurements at pH 5.5 and 6.5, the solution contained 4 mM Mes and pH was adjusted using Tris. In some solutions, NaCl was replaced by choline chloride. Potassium was omitted from all solutions to minimize effects on the endogenous oocyte Na+–K+ pump activity, which is highly sensitive to changes in extracellular [K+] (Cougnon et al. 1996). Oocytes were clamped to –30 mV with a DAGAN CA-1 amplifier and currents were measured after stepping from the holding potential to different test potentials with pCLAMP6.0 software. Short pulses (< 200 ms) were used to repress slowly activating endogenous currents. Pulses were given from +40 to –160 mV in 20 mV steps. Oocytes were placed in a small recording chamber and the gravity-driven solution exchange system allowed full exchange of bath solutions within a few seconds. Ammonium was applied for brief periods of < 2 min, and oocytes were immediately washed with standard bath solution. Data were collected from more than eight batches of oocytes. Each reported experimental result was observed in at least two independent batches of oocytes.
Data analysis
Data were processed using Sigma plot (Jandel Scientific). The available concentration of the charged or the uncharged form was calculated using the Henderson-Hasselbach equation: pH = pKa+ log10[NH3]/[NH4+]. A pKa of 9.25 was used for ammonium and a pKa of 10.66 was used for methylammonium. All data are given as means ± standard deviation. The concentration dependence of methylammonium uptake was fitted using V=Vmax/(1 +Km/c), where Vmax is maximal uptake at a saturating methylammonium concentration, Km is substrate concentration permitting half-maximal uptake, and c is the experimentally used concentration.
Radiotracer uptake
The ammonium analogue methylammonium (MA; [14C]CH3-NH3+; Amersham) was used as a radiotracer. Choline-based bath solutions were used for uptake experiments. Batches of > 10 healthy looking oocytes were incubated for 20 min in 200 µl of standard bath solutions containing radioactive methylammonium at room temperature. Initial tests showed that uptake did not saturate within the first 20 min (Westhoff et al. 2002). Oocytes were then carefully washed 5 times in 1 ml ice-cold buffer containing 10 mM unlabelled methylammonium and separated into groups of three per scintillation vial. After solubilization in 10 µl 5% SDS, 4 ml scintillation buffer was added and activity was analysed by liquid scintillation counting.
Intracellular pH changes (pHi)
Intracellular pH changes (pHi) were monitored using the pH-sensitive dye 2',7'-bis-(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) (Sasaki et al. 1992). Six to eight healthy oocytes were selected and placed in light-protected 1 ml uptake buffer (ND96, containing 5 µM BCECF-AM) and incubated at room temperature for 30 min. Two BCECF-loaded oocytes were washed in ND96, placed on a glass slide, covered with a coverslip and then placed on an inverted Leica fluorescence microscope. Oocytes were superfused with ND96 or ND96 containing 500 µM NH4Cl by gravity flow. Changes in BCECF fluorescence were measured using a spinning wheel fluorescence system with excitation at 410 ± 30 and 470 ± 20 nm and emission at 535 ± 20 nm. Pictures were taken every 10 s through an objective lens (x20) focused on the oocyte rim. Fluorescence changes obtained by superfusion of ND96 solution buffered to different pHo (supplemented with 10 µM of the protonophore carbonyl cyanide 3-chlorophenylhydrazone (CCCP) to collapse pHi) were used to determine the pHi/(fluorescence ratio) slope. CCCP was diluted from a 1000x stock in ethanol.
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As oocytes are known to express endogenous NH4+-permeable channels, they were first tested for endogenous ion channel activities measured between +20 and –140 mV. After oocytes had been voltage clamped, currents elicited by brief pulses were nearly identical in choline-based or sodium-based solution at pHo 7.5 (Fig. 1A). Addition of 1 mM ammonium did not change the currents, but a current of slightly varying magnitude appeared consistently upon addition of 
3 mM NH4+ (Fig. 1A).
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Methylammonium does not induce currents in RhBG-expressing oocytes
In order to minimize the effects of endogenous oocyte ammonium currents, the commonly used transport analogue methylammonium (H3C-NH3+, pKa= 10.66) was used. Methylammonium does not activate endogenous currents at low (mM) concentrations (Fig. 2; data not shown). In non-injected control oocytes, currents were similar if recorded at pHo 5.5 or pHo 8.5 and addition of 1 mM methylammonium did not change the current magnitude at either pHo. Inward currents recorded from RhBG-expressing oocytes were slightly larger, but were again similar at both pHo values. Addition of methylammonium did not induce electrical currents (Fig. 2).
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Methylammonium has the additional advantage that it can be used as a radiolabelled tracer. Uptake of [14C]methylammonium was first measured in non-injected oocytes. Methylammonium uptake (1 mM) increased at higher pHo values and higher H3C-NH2 concentrations in accordance with lipid diffusion of the uncharged species (Fig. 3). Uptake rates were several-fold higher at each pHo in RhBG-expressing oocytes (Fig. 3). RhBG-mediated methylammonium transport was calculated by subtracting endogenous background uptake. RhBG-mediated [14C]methylammonium transport increased at higher pHo (Fig. 3). Such pHo dependence would be expected for a transporter that exchanges H3C-NH3+ with H+, as the driving force for H+, and thus the driving force for the coupled transport, increases at alkaline pHo. This pHo dependence, however, might also be expected for a transporter that solely transports uncharged H3C-NH2, since the substrate concentration increases at higher pHo.
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At pHo 7.5, [14C]methylammonium uptake increased 7-fold upon RhBG expression, and was similar in sodium- or choline-based solutions (Fig. 4A). Thus the small sodium current described above (Fig. 1) is stoichiometrically uncoupled to methylammonium uptake. Uptake of [14C]methylammonium by RhBG-expressing oocytes was again calculated by subtracting endogenous background and saturated with a KmH3C – NH3+= 2.6 mM for charged H3C-NH3+ (Fig. 4B). Ammonium efficiently suppressed [14C]methylammonium uptake by RhBG, suggesting that ammonium is the natural substrate of RhBG and binds with a similar affinity (Fig. 4B and C). Ammonium inhibited [14C]methylammonium uptake with a KiNH4+= 3 mM (Fig. 4C). If the uncharged species (H3C-NH2) is the substrate transported by RhBG, its affinity can be recalculated for experiments at constant pHo 7.5, which gives KmH3C – NH2= 1.8 µM. Experiments at constant H3C-NH3+ (1 mM), but different pHo values, yield KmH3C – NH2= 0.4 µM (Fig. 4D).
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Ammonium, not methylammonium, is likely to be the natural substrate of RhBG and the previous results suggest that electroneutral NH3 transport or NH4+–H+ counter-transport is the mechanism involved. Ammonium transport was directly measured using the pH-sensitive dye 2',7'-bis-(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). If RhBG mediates net NH3 transport (either as NH3 transport or NH4+–H+ counter-transport), pHi alkalinization is expected. In contrast, if NH4+ is significantly transported, pHi acidification would be expected. Brief exposure to 500 µM NH4Cl induced a fluorescence ratio change in RhBG-expressing oocytes, but not in non-injected controls (Fig. 5), which suggested pHi alkalinization. The fluorescence ratio change was in the range of 0.15 ± 0.04 pHi units, as approximated by control measurements using calibrated pHo solutions in the presence of the protonophore CCCP. These experiments identify ammonium as the natural substrate of RhBG and do not support significant net NH4+ transport by RhBG. However, the results do not allow us to distinguish between NH4+–H+ counter-transport and NH3 transport, since both mechanisms reduce intracellular H+ concentrations, either by H+ efflux or, if NH3 is transported, by intracellular protonation of NH3 which consumes a H+ to form NH4+.
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Discussion |
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Electroneutral methylammonium transport by RhBG seems at first to be inconsistent with the fact that expression of RhBG in oocytes induced NH4+ currents (Fig. 1). However, it is well known that analyses of ammonium transporters in oocytes are hampered by the occurrence of distinct endogenous ammonium-activated, NH4+-permeable channels. Activation of these channels is preceded by diffusive entry of NH3, followed by internal alkalinization (Burckhardt & Fromter, 1992; Cougnon et al. 1996; Burckhardt & Burckhardt, 1997; Boldt et al. 2003). The appearance of such currents is dependent on millimolar external ammonium, while low (µM) concentrations do not activate these channels (Ludewig et al. 2002, 2003; Bakouh et al. 2004). The threshold of activation at pH 7.5 is just above 1 mM external ammonium (Ludewig et al. 2002; Boldt et al. 2003; Bakouh et al. 2004). Expression of any net NH3 transporter will increase ammonium entry, and hence internal alkalinization, and will thus lower the external ammonium concentrations needed to activate endogenous NH4+-permeable channels. As a consequence, it follows that even expression of an electroneutral net NH3 transporter inevitably leads to activation of endogenous NH4+ currents. This hypothesis may need further substantiation in future work, and as long as no specific inhibitor exists, it may remain speculative whether the NH4+ currents are carried by endogenous channels or in part of the RhBG protein itself. However, endogenous currents are largely suppressed at low pH, since the ammonium concentration needed to activate endogenous NH4+ currents is much higher (Boldt et al. 2003; Bakouh et al. 2004). Support for the hypothesis that the NH4+ currents are carried by endogenous channels comes from the fact that 1 mM ammonium did not induce currents in RhBG-expressing oocytes at pH 5.5 (data not shown). Activation of endogenous currents may also explain why a GFP-fused RhCG protein appeared to transport NH3 and NH4+ at the same time (Bakouh et al. 2004). Another preliminary study has identified ammonium-induced currents mediated by RhBG (Nakhoul & Hamm, 2004). Clearly, a more rigorous analysis of the endogenous NH4+-permeable channels and their relationship to Rh protein expression is needed.
Net NH3 transport by RhBG was monitored using the fluorescent dye BCECF. No changes were observed upon application of 500 µM NH4Cl in non-injected controls, while a pHi increase was observed in RhBG-expressing oocytes. Other methods to determine pHi, such as pH-sensitive impalement electrodes have identified a transient alkalinization followed by sustained acidification of pHi (Burckhardt & Fromter, 1992; Cougnon et al. 1996). A disadvantage of conventional pH-sensitive electrodes may be that the impalement depth is hard to quantify and pH-sensitive electrodes may measure bulk cytosolic pHi rather than the submembraneous pHi. As the oocyte cytoplasm is not highly transparent, BCECF probably measures the pHi close to the surface.
Methylammonium appears to be superior to ammonium as substrate for evaluating the transport mechanism and was thus mainly used in the present study to investigate RhBG function. RhBG transports methylammonium in an electroneutral manner, since at the resting potential of –20 mV, the uptake of 20 pmol min–1 would correspond to methylammonium currents of 32 nA, if the charged species is solely transported. As shown in Fig. 2, such a current is absent, indicating an electroneutral transport of methylammonium. Increased transport at higher pHo and higher H3C-NH2 concentrations may superficially support the conclusion that RhBG transports the uncharged substrate, but it should be noted that electroneutral NH4+–H+ exchange explains the data in Fig. 3 equally well. Different pHo not only change H3C-NH2 concentrations, but also change pH gradients across the oocyte membrane.
RhBG is a member of the AMT/MEP/Rh family of NH4+ transporters (Marini et al. 1997; Ludewig et al. 2001). Proteins of this family have been grouped into three branches according to sequence similarity: AMT-, MEP- and Rh-like proteins (Ludewig et al. 2001). Despite unambiguous evidence for NH4+ transport by bacterial and plant AMT/MEP-type proteins (Meier-Wagner et al. 2001; Ludewig et al. 2002), it has recently been suggested that NH4+ transporters are in fact channels for the gas NH3 (Soupene et al. 2002). This is surprising, as no direct binding or transport measurements have been presented to support the hypothesis (Soupene et al. 2002). In our hands, the growth tests in batch culture performed by Soupene et al. (2002) do not distinguish between NH4+ and NH3 transport. However, the many detailed studies on NH4+ transporters in bacteria, fungi and plants seem to provide unambiguous evidence that a general feature of these proteins is to bind and transport charged NH4+. While most studies used the NH4+ analogue methylammonium (Siewe et al. 1996; Marini et al. 1997; Meier-Wagner et al. 2001; Sohlenkamp et al. 2002), recent direct electrophysiological measurements of plant NH4+ transporters expressed in oocytes showed that the electrically charged NH4+ binds to the transporter and is transported (Ludewig et al. 2002, 2003). Interestingly, neither binding of NH4+ to that site, nor NH4+ transport is affected by thousand-fold changes in NH3 (Ludewig et al. 2002). Thus NH3 does not seem to bind the NH4+ transporter LeAMT1;1. This fact may not be too surprising, as NH4+ and NH3 are chemically distinct molecules and may require different binding sites: in addition to their charge difference, NH4+ has a tetrahedral structure with 109.5 deg bond angle, whereas NH3 has a pyramidal structure with 107 deg bonds.
It may be of minor importance in liver and kidney physiology whether RhBG exchanges NH4+–H+ or directly transports NH3. Although a final convincing biophysical demonstration for either suggestion may still be lacking, there are several arguments in favour of NH4+–H+ exchange. Homologous proteins (AMT/MEP/Rh family) often have similar binding sites, and in accordance with that, the affinity of H3C-NH3+ for RhBG is in a similar range to its affinity for LeAMT1;2, a NH4+ transporter from tomato (4-fold lower at –20 mV; Ludewig, U unpublished). Saturating binding sites are a typical feature of transporters and a binding site for charged NH4+ may be a structural feature of these structurally related proteins. As discussed above, NH3 does not influence binding of NH4+ to LeAMT1;1, and thus does not itself seem to bind to LeAMT1;1 (Ludewig et al. 2002). Binding of NH4+ to the related RhAG transporter is identical at different pHo values, but when it is assumed that the uncharged species binds, the binding constant varies 10-fold (Westhoff et al. 2002). The calculated affinity of uncharged H3C-NH2 for RhBG would be 1 µM. Such a high affinity seems to be inconsistent with a gas channel, as channels generally allow only loose binding to establish high flux rates (Hille, 2001). In addition, the binding constant of a substrate to its transporter should not vary under different conditions. However, when RhBG is assumed to bind and transport the uncharged substrate H3C-NH2, the affinities assayed at various pHo values or at constant pHo differ by a factor of 4.5 (Fig. 4D). As suggested for RhBG by the data presented in this study, electroneutral net NH3 transport has also been suggested for RhAG and RhCG transport proteins (Marini et al. 2000; Ludewig et al. 2001; Westhoff et al. 2002).
How do the results of this paper relate to the physiological role of RhBG? Electroneutral ammonium transport by RhBG is important in the liver, where two distinct metabolic pathways for ammonium exist (Haussinger et al. 1992). Periportal or midzonal hepatocytes mediate low-affinity ammonium absorption and metabolism to urea. These cells do not express significant amounts of either RhBG or RhCG. Perivenous hepatocytes, however, express basolateral RhBG in combination with a high-affinity, low-capacity metabolic pathway that converts ammonium to glutamine (Weiner et al. 2003). This suggests a major function of RhBG in uptake and scavenging of ammonium, and in hepatocyte pH regulation, probably in combination with bicarbonate transporters.
In the kidney, with few exceptions, those cells that express basolateral RhBG also express apical RhCG (Quentin et al. 2003; Verlander et al. 2003), suggesting that both transporters are corporately involved in transepithelial net NH3 secretion. RhBG and RhCG appear strongly expressed in acid-secreting intercalated cells in the collecting duct, which also express an apical H+-V-type ATPase. Active proton secretion may drive apical NH4+–H+ exchange and will concentrate NH4+ in the urine (Star et al. 1987; Wall et al. 1991). Basolateral RhBG may exchange NH4+ and H+ according to the gradients actively established at the opposite apical membrane and will, in the absence of a basolateral H+ leak, lead to acidification of the blood during transepithelial ammonium transport. Strong expression of RhBG and RhCG in acid-secreting intercalated cells are thus likely to maximize the efficiency of transepithelial ammonium secretion.
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X. Li, S. Jayachandran, H.-H. T. Nguyen, and M. K. Chan Structure of the Nitrosomonas europaea Rh protein PNAS, December 4, 2007; 104(49): 19279 - 19284. [Abstract] [Full Text] [PDF]
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R. M. Seshadri, J. D. Klein, S. Kozlowski, J. M. Sands, Y.-H. Kim, K.-H. Han, M. E. Handlogten, J. W. Verlander, and I. D. Weiner Renal expression of the ammonia transporters, Rhbg and Rhcg, in response to chronic metabolic acidosis Am J Physiol Renal Physiol, February 1, 2006; 290(2): F397 - F408. [Abstract] [Full Text] [PDF]
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R. Chambrey, D. Goossens, S. Bourgeois, N. Picard, M. Bloch-Faure, F. Leviel, V. Geoffroy, M. Cambillau, Y. Colin, M. Paillard, et al. Genetic ablation of Rhbg in the mouse does not impair renal ammonium excretion Am J Physiol Renal Physiol, December 1, 2005; 289(6): F1281 - F1290. [Abstract] [Full Text] [PDF]
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M. E. Handlogten, S.-P. Hong, L. Zhang, A. W. Vander, M. L. Steinbaum, M. Campbell-Thompson, and I. D. Weiner Expression of the ammonia transporter proteins Rh B glycoprotein and Rh C glycoprotein in the intestinal tract Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G1036 - G1047. [Abstract] [Full Text] [PDF]
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C. Lopez, S. Metral, D. Eladari, S. Drevensek, P. Gane, R. Chambrey, V. Bennett, J.-P. Cartron, C. Le Van Kim, and Y. Colin The Ammonium Transporter RhBG: REQUIREMENT OF A TYROSINE-BASED SIGNAL AND ANKYRIN-G FOR BASOLATERAL TARGETING AND MEMBRANE ANCHORAGE IN POLARIZED KIDNEY EPITHELIAL CELLS J. Biol. Chem., March 4, 2005; 280(9): 8221 - 8228. [Abstract] [Full Text] [PDF]
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) and sodium-based solutions without ammonium (•), or containing 1 mM (
) and 20 mM (
) ammonium. A, background currents from non-injected oocytes (n= 7). B, means from (n= 5) RhBG-expressing oocytes. C, effect of extracellular cations on inward currents at –100 mV (n.i. = not injected).
pHH3C – NH2= 0.4 µM.