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1 Division of Structural Cell Biology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
2 Medicinal Pharmacology Laboratory, Medicinal Research Laboratories, Taisho Pharmaceutical Co., Ltd, 1-403 Yoshino, Kita-ku, Saitama, Saitama 331-9530, Japan
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Abstract |
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(Received 13 September 2005;
accepted after revision 24 November 2005;
first published online 24 November 2005)
Corresponding author S. Shiosaka: Division of Structural Cell Biology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan. Email: sshiosak{at}bs.naist.jp
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Introduction |
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There is growing evidence that adhesion molecules play a critical role in stabilizing LTP, with interactions between synaptic membranes and the extracellular matrix (ECM) being particularly important. Ectodomain proteolysis of matrix and cell adhesion molecules (CAM) may play significant roles in dynamic synaptic rearrangement and mechanical plasticity (Matsumoto-Miyai et al. 2003). Several lines of evidence support the possibility that interactions between synaptic membranes and the ECM participate in certain types of synaptic plasticity, such as kindling, kainite-induced seizures, and LTP. Indeed, LTP is reduced by application of peptides and antibodies that block the extracellular interactions of adhesion receptors belonging to the CAM and integrin families (Luthl et al. 1994; Ronn et al. 1995; Bahr et al. 1997; Staubli et al. 1998). Moreover, tissue plasminogen activator (tPA), matrix metalloproteinases, and other extracellular proteases, either released or constitutively present, have been linked to LTP (Frey et al. 1996; Huang et al. 1996; Baranes et al. 1998). Neuropsin (NP; also referred to as KLK8) is of particular interest in this regard because of its unusual distribution and high concentration in the forebrain (Chen et al. 1995). This protease degrades the presynaptic L1 cell adhesion molecule (L1 cam) in an NMDA receptor-dependent fashion (Matsumoto-Miyai et al. 2003) and could thus serve as a link between the postsynaptic triggers for LTP and presynaptic adhesion changes needed for stable potentiation. There is also evidence that NP is involved in early phases of LTP (Momota et al. 1998; Yoshida & Shiosaka, 1999; Komai et al. 2000), unlike tPA, which predominantly affects L-LTP (Frey et al. 1996; Huang et al. 1996; Baranes et al. 1998), possibly via an action of the presynaptically located L1; the shedding of the extracellular domain of L1 is reported to play a significant role in establishing E-LTP (Matsumoto-Miyai et al. 2003).
These results strongly suggest that cleavage of an adhesion molecule by NP may be involved in converting sequential enhancement of a signalling pathway during E-LTP into stable memory acquisition. However, Davies et al. (2001) reported that NP-knockout mice had no significant impairment of spatial learning and displayed normal hippocampal LTP. In the present study therefore we re-examined behavioural memory and electrophysiological synaptic plasticity in NP-knockout mice, and then evaluated whether the protease-activated system alone can evoke potentiation that shares the same signalling mechanism with LTP. We found that learning and E-LTP were significantly impaired in NP-knockout mice, and that NP alone is necessary for the establishment of E-LTP.
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Methods |
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A total of 156 male ddY mice (9–12 weeks old) and 45 male NP+/+, +/– and –/– mice (8–22 weeks old) were used in the present study. ddY mice were purchased from SLC Co. (Hamamatsu, Japan), and heterozygous and homozygous NP-mutant mice were produced as previously described (Hirata et al. 2001). NP–/– mice were backcrossed to C57BL/6J (n > 9), and C57BL/6J control mice (littermates) were used. Mice were maintained under a 12 h: 12 h light: dark cycle and fed ad libitum. At the end of the physiological experiments, mice were killed with an overdose of pentobarbital. All experimental protocols described below were performed according to the animal care and experimentation guidelines of the Nara Institute of Science and Technology and Physiological Society of Japan.
Morris water maze
A circular pool (diameter 120 cm, height 30 cm) was filled to a depth of 15 cm with water at room temperature (21–22°C). Four equally spaced points around the edge of the pool were designated as four starting positions: east (E), south (S), west (W) and north (N). A hidden circular platform (diameter 8 cm) was set 0.5 cm below the surface of the water in a fixed position in the middle of the NW quadrant. Mice were trained to find the platform for four consecutive days. Training consisted of four trials per day with an intertrial interval of 120 min. The mice were placed in the water, facing the wall, at one of the four starting positions in a random sequence; each position was used once each day. The time required to find the escape platform (escape latency) was measured during each trial. Having found and climbed onto the platform, the mice stayed there for 30 s and were then returned to their home cage. If the mice failed to find the platform within 100 s, they were placed on the platform by the experimenter and a maximum score of 100 s was assigned.
Y-maze
The Y-maze apparatus consisted of three arms. Each arm was 40 cm long, 10 cm high, 3 cm wide at the bottom and 10 cm wide at the top, and converged in an equilateral triangular central area. Each mouse, naive to the maze, was placed at the end of one arm and allowed to move freely through the maze during an 8 min session. The sequence of arm entries was recorded manually. Entry was considered complete when the hind paws of the mouse had completely entered the arm. A spontaneous alternation behaviour, which is regarded as a measure of spatial memory, was defined as successive entries into the three different arms (A, B or C) in overlapping triplet sets (e.g. ACBA BACBAB = 5). Percentage alternation was calculated as the ratio of actual to possible alternation (defined as the total number of arm entries minus two) multiplied by 100, as shown in the following equation:
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The number of arm entries also serves as an indicator of activity.
Electrophysiology
ddY male mice were anaesthetized with urethane (1.25 g kg–1; I.P.) and placed in a stereotaxic frame with the skull horizontal. Rectal temperature was maintained at 37°C using a heated jacket pad (BWT-100, Bio Research Center, Nagoya, Japan). Extracellular field recording was carried out as previously described (Matsumoto-Miyai et al. 2003). A bipolar stainless-steel electrode (InterMedical Co., Tokyo, Japan) was used for stimulation in the two populations of Schaffer collateral–commissural projections (2.46 mm posterior and 2.30 mm lateral to bregma or 2.46 mm posterior and 0.4 mm lateral to bregma). Test pulses (100 µs duration) were collected every 30 s, and their intensity was adjusted to evoke field excitatory postsynaptic potential (fEPSP) and population spikes which were 
50% of the maximal responses. Both were recorded with a monopolar tungsten electrode (InterMedical Co.) placed in the stratum radiatum or the stratum pyramidale of the CA1 region (2.46 mm posterior and 2.0 mm lateral to bregma). The slope of fEPSP was measured on the maximal initial slope, and the population spike amplitude was evaluated as the difference between the average of two positive peaks and the negative peak. All responses were expressed as a percentage change from the average response recorded during the 30 min immediately before inhibitor, antibody or r-NP injection, or LTP-inducing stimuli. E-LTP was induced using a single tetanic stimulation (100 Hz for 1 s). A theta-burst stimulation (TBS) protocol consisting of 10 burst-like trains (10 pulses at 200 Hz) at 5 Hz, applied four times every 30 s, was used in an attempt to elicit LTP. Long-term depression (LTD) was induced by low-frequency paired stimulation (LFPS; 700 µA, 150 µs pulses), comprising 900 pairs of pulses given at an interstimulus interval of 50 ms and an interpair interval of 1 s (Thiels et al. 1994). Paired-pulse facilitation (PPF) was tested after a 20 min period of stable responses and 3 h after terminating r-NP injection. Interpulse intervals of 25, 50, 75, 100 and 200 ms were investigated.
Administration of protease inhibitor, anti-NP antibody and r-NP
Intracerebroventricular infusion of an NP-specific inhibitor, NP stop (Taisho Pharmaceutical Co., Tokyo, Japan), and an activity-neutralizing anti-NP antibody (mAbB5; MBL, Nagoya, Japan) was performed as reported previously (Momota et al. 1998). NP stop (1 µg ml–1), mAbB5 (1.4 µg ml–1) or rat IgG diluted in saline was injected (0.22 mm anterior and 1.0 mm lateral to bregma; 2.3 mm ventral from surface of skull) at flow rates of 0.05 µl min–1 for 40 min or 0.5 µl min–1 for 10 min, using a Hamilton syringe driven by syringe pump (CFV-2100; Nihon Kohden, Tokyo, Japan). The r-NP was prepared as previously described (Shimizu et al. 1998); r-proNP, an inactive precursor form, was first obtained from insect cells (Shimizu et al. 1998) and then activated by lysyl endopeptidase (Kato et al. 2001). The enzymatic activity of r-NP was measured with t-Butyloxycarbonyl-L-Valyl-L-Prolyl-L-Arginine 4-Methyl-Coumaryl-7-Amide (Boc-Val-Pro-Arg-MCA), one unit being defined as the amount required to hydrolyse 1 µmol of this substrate per minute at 30°C. The r-NP was diluted with artificial cerebrospinal fluid (ACSF) consisting of 127 mM NaCl, 1.6 mM KCl, 1.24 mM KH2PO4, 1.3 mM MgSO4, 2.4 mM CaCl2, 26 mM NaHCO3 and 10 mM glucose. ACSF containing r-NP, or an equal volume of ACSF alone, was delivered for 10 min at a rate of 80 nl min–1 with a 10 µl Hamilton syringe in the hippocampus. For control experiments, r-NP (1.8 mU ml–1) was irreversibly inactivated by overnight incubation at 4°C with 3 mM phenyl methane sulphonyl fluoride (PMSF) (a serine protease inhibitor) or 5 µg ml–1 mAbB5, and used instead of active r-NP. The Hamilton syringe was connected to the infusion cannula with polyethylene tubing.
Western blot analysis
Hippocampi were removed from three ddY mice 90 min after r-NP injection, and the CA1 region around the inserted electrodes was microdissected. These samples were homogenized in a glass-Teflon homogenizer in ice-cold homogenization buffer (5 mM Hepes, pH 7.4, 320 mM sucrose, 5 mM EDTA, 5 mM EGTA, 50 mM NaF, 10 mM sodium pyrophosphate, 1 mM sodium orthovanadate, 1 mM PMSF, 1 µM okadaic acid and 5 µg ml–1 leupeptin), and then incubated on ice for 10 min. The homogenates were centrifuged at 1000 g for 10 min at 4°C to pellet the nuclei. The supernatants were centrifuged at 10 000 g for 15 min at 4°C, and the crude synaptosomal pellets were resuspended in homogenization buffer and centrifuged again at 10 000 g for another 15 min at 4°C. The washed crude synaptosomes were suspended in lysis buffer (50 mM Tris-HCl, pH 6.8, 150 mM NaCl, 5 mM EDTA, 5 mM EGTA, 50 mM NaF, 10 mM sodium pyrophosphate, 1 mM sodium orthovanadate, 1 mM PMSF, 1% SDS, 1 µM okadaic acid and 5 µg ml–1 leupeptin). Protein concentration was determined by BCA assay (Pierce, Rockford, IL, USA). Equal amounts of proteins were loaded onto SDS-PAGE gels (7.5%), and were then transferred to polyvinylidene difluoride (PVDF) membranes. Western blotting for the phosphorylated GluR1 subunit was performed as previously described (Lee et al. 1998). Briefly, the blotted PVDF membranes were blocked with 4% BSA and 0.05% Tween-20 in PBS for 1 h, and then incubated for 2 h with phosphorylation site-specific antibodies (1: 300; a gift from Dr K. Kameyama, National Institute of Bioscience and Human Technology, Japan). After being washed five times for 5 min each with 1% BSA and 0.05% Tween-20 in PBS, the membranes were incubated for 1 h with alkaline phosphatase-conjugated anti-rabbit immunoglobulin (1: 2000). Immunoreactive bands were detected using the Immun-Star chemiluminescent system (Bio-Rad, Hercules, CA, USA) and exposure to X-ray film. The antibodies were stripped with 62.5 mM Tris-HCl, pH 6.8, 2% SDS, and 100 mM 2-mercaptoethanol at 60°C for 30 min, after which the membranes were reblocked and reprobed with anti-C-terminal GluR1 antibodies (Upstate Biotechnology, Lake Placid, NY, USA) to estimate the total amount of GluR1. Band densities were measured using Scion Image (Scion Co., Frederick, MD, USA). Phospho-GluR1 immunoreactivity was normalized to total GluR1 using a phosphorylation-independent antibody.
Data analysis
All graphical data are represented as the mean ±S.E.M. Statistical analysis of group effects was performed using one-way or two-way analysis of variance (ANOVA) followed by Scheffé's test for post hoc comparison, and individual effects were measured by the unpaired t test. Significance was set at P < 0.05.
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Results |
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We evaluated hippocampus-dependent learning of NP-mutant mice in the Morris water maze task. During the hidden platform task, the escape latency period for the knockout mice was significantly longer than that for wild-type mice (two-way ANOVA: F(1,21)= 6.22, P= 0.021) and the latency period for heterozygous mice tended to be longer than for wild-type mice (two-way ANOVA: F(1,18)= 4.19, P= 0.056) (Fig. 1A and B). In a visual task, there was no difference between the knockout and the wild-type mice (data not shown).
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Despite the significant impairments in the learning tasks shown by the knockout mice, no difference was seen among NP–/–, NP+/– and NP+/+ mice in spontaneous motor activity examined by an infrared beam-based activity sensor apparatus during the 120 min test period (data not shown).
Deletion of the NP gene or inhibition of NP activity abolishes tetanus-elicited E-LTP and reduces theta-burst-elicited LTP in vivo
To examine the synaptic effects of NP, we first analysed hippocampal LTP using NP-deficient mice. E-LTP was severely impaired and almost disappeared in the homozygous mutants following a single tetanus, an E-LTP-specific stimulus (60 min after tetanus: wild-type, 120.5 ± 4.5% of baseline, n= 6; NP-knockout, 100.5 ± 2.1% of baseline, n= 6; t test, P < 0.003; Fig. 2A). Since NP is known to be activated during LTP in an NMDA receptor-dependent manner (Matsumoto-Miyai et al. 2003), we further determined whether inhibition of NP activity can abolish an induced LTP using the activity-neutralizing anti-NP monoclonal antibody mAbB5 or the NP-specific inhibitor NP stop. E-LTP was completely blocked by an intracerebroventricular infusion of either mAbB5 (60 min after tetanus: mAbB5, 103.7 ± 3.5% of baseline, n= 3; rat IgG, 125.1 ± 2.9% of baseline, n= 3; t test, P < 0.02) or NP stop (60 min after tetanus: NP stop, 100.5 ± 1.8% of baseline, n= 5; saline, 126.6 ± 4.3% of baseline, n= 5; t test, P < 0.0006; Fig. 2B). Consistent with our previous data (Komai et al. 2000), application of mAbB5 after 30 min of baseline recording and before TBS resulted in a significant decrease in the magnitude of LTP, most markedly in the early phase (data not shown). This was also confirmed by infusion of NP stop, where a significant TBS-associated reduction in LTP was found (90 min after TBS: NP stop, 114.7 ± 1.9% of baseline, n= 8; saline, 140.2 ± 6.6% of baseline, n= 6; t test, P < 0.002; Fig. 2C). Therefore, endogenous NP, and specifically its proteolytic activity, may play a critical role in the induction of E-LTP in vivo. NP stop, mAbB5 and rat IgG did not have any effect on baseline synaptic transmission.
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If NP has significant effects on E-LTP, exogenous application of recombinant (r-) NP alone should elicit LTP. The experiment was designed because the proteolytic activity of NP (which is usually inactive) is transiently activated by LTP-inducible neural stimuli and can be substituted by an exogenously applied active form of r-NP (Shimizu et al. 1998; Komai et al. 2000; Matsumoto-Miyai et al. 2003). Therefore, we measured the population spike amplitude and the fEPSP slope after intrahippocampal infusion of r-NP in vivo. Infusion led to an increase of the fEPSP slope (180 min after application of 1.8 mU ml–1 r-NP: 137.5 ± 5.8% of baseline, n= 5; Fig. 3A) and of the population spike amplitude in CA1 pyramidal neurones (180 min after application of 1.8 mU ml–1 r-NP: 191.7 ± 7.0% of baseline, n= 5; Fig. 3B). The enhancement reached a plateau 2.5 h after the injection, and was then sustained up to at least 8 h (480 min after application of 1.8 mU ml–1 r-NP: 145.2 ± 0.59% of baseline, n= 3; Fig. 3C). Neither PMSF- nor mAbB5-inactivated r-NP (see Methods) enhanced synaptic efficacy (180 min after application of 1.8 mU ml–1 r-NP: PMSF, 100.0 ± 0.47% of baseline, n= 3; Fig. 3D) (180 min after application of 1.8 mU ml–1 r-NP: mAbB5, 104.6 ± 1.7% of baseline, n= 4).
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The effect of r-NP on the fEPSP slope was examined by infusion of low (0.18 mU ml–1), medium (1.8 mU ml–1) and high (3.6 mU ml–1) concentrations of r-NP. r-NP significantly changed the basal synaptic transmission in a dose-dependent manner (F(3,14)= 25.3, P < 0.001). The low dose had less enhancing effect on fEPSP than the medium dose (60 min after application of a low dose of r-NP: 112.3 ± 1.8% of baseline, n= 5; Fig. 3E and F). Intriguingly, the high dose of r-NP had the opposite effect to the low and medium doses, significantly depressing synaptic transmission (60 min after application of a high dose of r-NP: 85.0 ± 3.8% of baseline, n= 4; one-way ANOVA, P < 0.01 versus ACSF: 60 min after application of ACSF; 104.0 ± 1.1% of baseline, n= 4; Fig. 3E and F). Infused r-NP alone can therefore elicit not only LTP but also LTD in vivo, depending on the dose applied. Because the medium dose (1.8 mU ml–1) produced the maximum effect on LTP-like potentiation, this concentration was used for all subsequent experiments, unless otherwise stated.
Paired-pulse facilitation is not affected during r-NP-elicited synaptic potentiation
Paired-pulse facilitation (PPF) was examined to identify any presynaptic modification by infusing the medium dose of r-NP into the CA1 subfield. There was no difference between before and after r-NP injection at any of the interstimulus intervals tested (25, 50, 75, 100 and 200 ms, n= 9, two-way ANOVA, F(1,80)= 2.09, P= 0.15; Fig. 4A). The size of PPF taken before r-NP injection was largely consistent with that 3 h after injection (and reduction of stimulus intensity to match the pre-r-NP level) (Fig. 4B), indicating that the probability of neurotransmitter release is not affected by r-NP. Thus, synaptic potentiation by r-NP may be attributable to a change in postsynaptic gain.
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Since r-NP alone can elicit long-lasting synaptic potentiation or depression (Fig. 3E and F), we next focused on whether two forms of synaptic potentiation, electrically and r-NP-elicited, occlude each other. We monitored two populations of Schaffer collateral–commissural projections to the same population of postsynaptic neurones. LTP was induced using TBS in one of the pathways (S1), while the other pathway (S2) served as a control (Fig. 5A). Induction of stable LTP prevented the subsequent r-NP-induced synaptic potentiation, whereas application of r-NP led to an increase in the fEPSP slope on the control pathway (150 min after TBS: S1, 99.6 ± 3.5% of baseline, n= 5; S2, 133.1 ± 6.2% of baseline, n= 5, t test, P < 0.002 versus control; Fig. 5B).
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r-NP-elicited change in fEPSP is responsible for phosphorylation of GluR1 subunit of AMPA receptor
Western blot analysis using antibodies specific to the phosphorylated GluR1 subunit enabled us to examine LTP- or LTD-associated change in phosphorylation of the AMPA receptor (LTP: Ser-831; LTD: Ser-845). The phosphorylation profile of the receptor was examined after expression of r-NP-induced LTP- and LTD-like synaptic changes to verify a common cellular signalling mechanism for the two types of synaptic plasticity. As shown in Fig. 6, the medium dose of r-NP, which elicits maximum LTP-like potentiation, increased band density on Ser-831 (155.3 ± 13.8% of control phosphorylation, n= 5, t test, P < 0.004; Fig. 6) but not on Ser-845 (101.1 ± 7.0% of control phosphorylation, n= 5, t test, P > 0.8; Fig. 6). In contrast, the high dose of r-NP into the hippocampus, which induces LTD-like depression, significantly reduced band density on Ser-845 (58.9 ± 13.6% of control phosphorylation, n= 5, t test, P < 0.02; Fig. 6), with no change for Ser-831 (109.6 ± 8.1% of control phosphorylation, n= 5, t test, P > 0.2; Fig. 6). PMSF- or mAbB5-inactivated r-NP completely abolished the changes in phosphorylation of GluR1 (PMSF: Ser-831, 105.3 ± 6.0% of control phosphorylation, n= 3, t test, P > 0.4; Ser-845, 99.9 ± 2.2% of control phosphorylation, n= 3, t test, P > 0.9; mAbB5: Ser-831, 101.7 ± 6.3% of control phosphorylation, n= 3, t test, P > 0.7; Ser-845, 96.1 ± 6.1% of control phosphorylation, n= 3, t test, P > 0.5; Fig. 6). Taken together, these data show that r-NP-induced synaptic plasticity shares an identical cellular mechanism with conventional LTP and LTD elicited by electric stimuli.
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Discussion |
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However, there are some inconsistencies between our data and those of Davies et al. (2001) for NP-deficient mice. Their knockout mice displayed normal LTP (presumably L-LTP), established by repeated tetanic stimulation, and a normal performance in the water maze test, whereas our data indicate significant impairment in both electrophysiological and behavioural studies. The present study clearly demonstrates that the effects of NP on synaptic plasticity are most marked, perhaps even critical, in the early phase of LTP, but less significant in the late phase. Because NP deficiency, or infusion of NP stop or anti-NP monoclonal antibody, strongly impaired E-LTP induced by a single tetanus, it is reasonable to assume that NP is involved in E-LTP rather than L-LTP. The same tendency was also found in the behavioural tests: NP-knockout mice are impaired not in memory retention but in memory acquisition (present study). Since stronger stimuli preferentially elicited late-phase potentiation, the water maze task, which is relatively stressful for mice, induced rather mild impairment in knockout mice. Davies et al. (2001) did not observe any aberration in the water maze task. The contradictory results might be attributable to differences in experimental conditions. The water maze test is particularly sensitive to water temperature, for example: animals trained in cooler water (19°C) perform better than those trained in the warmer water (25°C) which Davies et al. (2001) used (Akirav et al. 2001), and we used relatively cool water (21–22°C) for the water maze task in the present study. Other factors contributing to the inconsistency might be genetic background, the way in which the NP locus was disrupted in deficient mice, and different experimental conditions. To minimize genetic background influences, we exclusively used mutant mice of backcross generation (n > 9) on C57BL/6J and their littermates as a control. We also examined another memory task in addition to the water maze task. Spontaneous alternation behaviour in the Y-maze is regarded as a spatial working memory test. NP–/– and NP+/– mice exhibited a lower alternation ratio than the wild-type mice. Thus, the results of both memory tasks strongly support the idea that NP is involved in the acquisition of hippocampus-dependent learning processes, but is not so important in retention of memory.
The application of NP had no significant effect on paired-pulse facilitation, meaning that NP does not have any effect on presynaptic transmitter release. Therefore, NP-elicited plasticity in the hippocampus is a postsynaptic event, as are electrically evoked LTP and LTD. This interpretation is strongly supported by occlusion experiments and by activation of the AMPA receptor at specific phosphorylation sites for LTP and LTD. Thus, NP-dependent synaptic modification downstream of stimulus-triggered NMDA receptor signalling returns again to the postsynaptic elements, without modification of presynaptic elements. This turn-back signalling from outside to inside might multiply or strengthen the postsynaptic potentiation of signals. Since NP is diffusible in the extracellular space, it can be speculated that neighbouring boutons of L1cam-positive immature small synapses and not-yet-contacted L1cam-positive immature boutons (orphan boutons) are oscillatorily activated during LTP (Nakamura et al. unpublished observations).
We found that the active form of the protease NP alone can elicit a plastic change of intracellular signalling which is similar to electrically evoked LTP, and acts as a major extracellular modifier, particularly on E-LTP. The results imply that postsynaptic signalling subsequent to E-LTP-inducible stimuli returns to the extracellular space, where proNP (zymogen) is ready to activate (Shimizu et al. 1998; Matsumoto-Miyai et al. 2003). Activation of NP occurs by cleavage of a 4-amino acid peptide (QGSK) after a signal from downstream of the NMDA receptor (Shimizu et al. 1998; Matsumoto-Miyai et al. 2003). It is clear that this activation of NP is required for establishment of E-LTP and also partially of LTP induced by TBS, because inhibition or inactivation of NP blocked E-LTP completely and LTP induced by TBS partially (Komai et al. 2000; present study).
Finally, the present study has clearly demonstrated that NP-driven synaptic modification, presumably due to rearrangement of the ECM, is a major mechanism for synaptic plasticity, particularly in E-LTP. This mechanism also plays an important role in LTD. Peri-synaptic proteolysis of the ECM by NP might be essential in E-LTP and consequently in acquisition of memory.
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) mice are shown on the top. B and C, NP inhibitor, NP stop, significantly blocked theta-burst LTP and completely blocked E-LTP elicited by a single train of tetanus. NP stop (•) or saline (
