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¹ Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA

	Abstract

Top Abstract Introduction Methods Results and Discussion References

 
We have investigated the nature of the Ca²⁺ entry supporting [Ca²⁺]_i oscillations in human embryonic kidney (HEK293) cells by examining the roles of recently described store-operated Ca²⁺ entry proteins, Stim1 and Orai1. Knockdown of Stim1 by RNA interference (RNAi) reduced the frequency of [Ca²⁺]_i oscillations in response to a low concentration of methacholine to the level seen in the absence of external Ca²⁺. However, knockdown of Stim1 did not block oscillations in canomical transient receptor potential 3 channel (TRPC3)-expressing cells and did not affect Ca²⁺ entry in response to arachidonic acid. The effects of knockdown of Stim1 could be reversed by inhibiting Ca²⁺ extrusion with a high concentration of Gd³⁺, or by rescuing the knockdown by overexpression of Stim1. Similarly, knockdown of Orai1 abrogated [Ca²⁺]_i oscillations, and this was reversed by use of high concentrations of Gd³⁺; however, knockdown of Orai1 did not affect arachidonic acid-activated entry. RNAi targeting 34 members of the transient receptor potential (TRP) channel superfamily did not reveal a role for any of these channel proteins in store-operated Ca²⁺ entry in HEK293 cells. These findings indicate that the Ca²⁺ entry supporting [Ca²⁺]_i oscillations in HEK293 cells depends upon the Ca²⁺ sensor, Stim1, and calcium release-activated Ca²⁺ channel protein, Orai1, and provide further support for our conclusion that it is the store-operated mechanism that plays the major role in this pathway.

(Received 27 November 2006; accepted after revision 8 January 2007; first published online 11 January 2007)
Corresponding author G. S. Bird: NIEHS, PO Box 12233, Research Triangle Park, NC 27709, USA. Email: bird{at}niehs.nih.gov

	Introduction

Top Abstract Introduction Methods Results and Discussion References

 
Activation of phospholipase C-coupled receptors results in both intracellular Ca²⁺ release and increased influx of Ca²⁺ across the plasma membrane. In many instances, the influx of Ca²⁺ occurs through the capacitative or store-operated route (Putney, 1986; 1997,; Parekh & Penner, 1997; Parekh & Putney, 2005). When supramaximal receptor activation induces large, sustained increases in [Ca²⁺]_i, the evidence for capacitative calcium entry is fairly straightforward. However, when lower, more physiological concentrations of receptor agonists induce regenerative [Ca²⁺]_i oscillations, both the role and nature of the associated Ca²⁺ entry is a matter of some debate. Specifically, it has been suggested that another, non-capacitative pathway involving arachidonic acid-activated channels provides the Ca²⁺ entry necessary to drive [Ca²⁺]_i oscillations (Shuttleworth, 1999; Shuttleworth et al. 2004). We recently published pharmacological evidence indicating that in human embryonic kidney (HEK293) cells, maintenance of [Ca²⁺]_i oscillations required capacitative or store-operated Ca²⁺ entry (Bird & Putney, 2005). However, the specificity of this pharmacological approach has been questioned (Mignen et al. 2005). In addition, in a recent report, Yan et al. (2006) found no effect of knockdown of the store-operated Ca²⁺ entry protein, Stim1, on [Ca²⁺]_i oscillations in intestinal cells of Caenorhabditis elegans. These investigators concluded that store-operated entry was not required for Ca²⁺ signalling under physiological conditions, but rather functioned in pathophysiological conditions of extreme store depletion (Yan et al. 2006). Therefore, in the current study, we utilized a molecular approach to investigate the Ca²⁺ entry mechanism involved in [Ca²⁺]_i oscillations in HEK293 cells. We utilized RNA interference (RNAi) to knock down two recently described molecular components of capacitative calcium entry, Stim1 (Roos et al. 2005; Liou et al. 2005) and Orai1 (Feske et al. 2006; Zhang et al. 2006; Vig et al. 2006b). In confirmation of the finding of Roos et al. (2005), knockdown of Stim1, but not the structurally related Stim2, significantly decreased store-operated Ca²⁺ entry, as assessed with both thapsigargin and a supramaximal concentration of methacholine. Knockdown of Stim1 also significantly reduced the frequency of [Ca²⁺]_i oscillations in response to low concentrations of methacholine. The specificity of Stim1 was demonstrated by the failure of Stim1 knockdown to reduce canomical transient receptor potential 3 channel (TRPC3)-dependent oscillations, or arachidonic acid-activated Ca²⁺ entry. Knockdown of the calcium release-activated calcium channel component, Orai1, similarly inhibited oscillations. These findings show that two proteins that play essential roles in capacitative calcium entry, Stim1 and Orai1, are essential in the signalling mechanism for [Ca²⁺]_i oscillations under conditions of physiological stimulation strengths. They also provide further support for our conclusion that the store-operated pathway provides the Ca²⁺ entry supporting [Ca²⁺]_i oscillations in HEK293 cells.

	Methods

Top Abstract Introduction Methods Results and Discussion References

 
Cell culture

HEK293 cells (ATCC) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and 2 mM glutamine and maintained in a humidified 95% air–5% CO₂ incubator at 37°C. HEK293 cells stably expressing a green fluorescent protein-tagged TRPC3 were also maintained in culture as described by Trebak et al. (2003). In preparation for cDNA transfection or small inhibitory RNA (siRNA) knockdown, cells were transferred to six-well plates and allowed to grow to 90% confluence. In preparation for Ca²⁺ measurements, cells were cultured to about 70% confluence and then transferred onto 30 mm round glass coverslips (#1 thickness) at two different cell densities (Bird & Putney, 2005). Specifically, 0.5 ml either a 400 000 cells ml^–1 (high density) or 60 000 cells ml^–1 (low density) cell suspension was transferred to the centre of the coverslip, and the cells were left to attach for a period of 12 h. Additional DMEM was then added to the coverslip, and the cells were maintained in culture for an additional 36 h before use for calcium measurements. Unless specified, cells were grown in the high density condition; as previously described, low density conditions were required for reproducible responses to arachidonic acid (Bird & Putney, 2005).

Plasmids

Stim1 with the enhanced yellow fluorescent protein (EYFP) fused to the N-terminus was obtained from Tobias Meyer, Stanford University. Full-length Stim2 cDNA plasmid was purchased from Origene in the pCMV6-XL5 vector, and EYFP-C1 from Clontech (Mountain View, CA, USA).

siRNA knockdown

HEK293 cells were plated in a six-well plate on day 1. On day 2, cells were transfected with siRNA (100 nM) against Stim1 (Dharmacon, Lafayette, CO, USA), Orai1 (Invitrogen) or siCONTROL (Dharmacon) using Metafectene (Biontex Laboratories GmbH, Martinsried/Planegg, Germany; 7 µl per well), and including siGLO (Dharmacon) as a marker. The sequence of the siRNA against Stim1 was: agaaggagcuagaaucucac (Mercer et al. 2006); for Orai1 it was: cccuucggccugaucuuuaucgucu (Mercer et al. 2006). After a 6 h incubation period, the medium bathing the cells was replaced by DMEM and maintained in culture. On day 3, siRNA-treated cells were optionally transfected with cDNA for Stim1 tagged with EYFP, Stim2 plus EYFP or EYFP alone as described below. On day 4, cells were transferred to 30 mm glass coverslips in preparation for Ca²⁺ measurements as described above, which were performed on day 5 or 6.

cDNA transfection

HEK293 cells were plated in a six-well plate on day 1. On day 2, cells were transfected using Lipofectamine 2000 (Invitrogen; 2 µl per well) with cDNA for EYFP, EYFP-tagged Stim1 or EYFP-tagged Stim2. After a 6 h incubation period, the medium bathing the cells was replaced by complete DMEM and maintained in culture. On day 3, cDNA-treated cells were transferred to 30 mm glass coverslips in preparation for Ca²⁺ measurements as described above, which were performed on day 4 or 5. The concentrations of plasmids used were 0.5 µg well^–1 for EYFP–Stim1, 0.1 µg well^–1 for EYFP and 2 µg well^–1 for Stim2.

Single-cell calcium measurement

Fluorescence measurements were made with HEK293 cells loaded with the calcium sensitive dye, fura-5F, as previously described (Bird & Putney, 2005). Briefly, cells plated on 30 mm round coverslips and mounted in a Teflon chamber were incubated in DMEM with 1 µM of the acetoxymethyl ester (AM) form of fura-5F (fura-5F/AM, Molecular Probes, USA) at 37°C in the dark for 25 min. For [Ca²⁺]_i measurements, cells were bathed in Hepes-buffered salt solution (HBSS) at room temperature (21°C) containing (mM): NaCl 120, KCl 5.4, Mg₂SO₄ 0.8, Hepes 20, CaCl₂ 1.8 and glucose 10; pH was adjusted to 7.4 with NaOH. Nominally Ca²⁺-free solutions were HBSS with no added CaCl₂. Fluorescence images of the cells were recorded and analysed with a digital fluorescence imaging system (InCyt Im2, Intracellular Imaging Inc., Cincinnati, OH, USA). Fura-5F fluorescence was monitored by alternatively exciting the dye at 340 and 380 nm, and collecting the emission wavelength at 520 nm. Changes in intracellular calcium are expressed as the ratio of fura-5F fluorescence due to excitation at 340 and 380 nm (F₃₄₀/F₃₈₀). Before starting the experiment, regions of interests identifying transfected cells expressing the EYFP tag were created by observing cells at a 530 nm emission wavelength and illuminated with 477 nm excitation light. Typically, 25–35 cells were monitored per experiment. In all cases, ratio values have been corrected for contributions by autofluorescence, which is measured after treating cells with 10 µM ionomycin and 20 mM MnCl₂. Quantitative analysis of agonist-induced oscillation rates was carried out as previously described (Bird & Putney, 2005). Rates of oscillation were calculated on responding cells only, and the fraction of responding cells is reported in each case. Responding cells were those producing at least one immediate transient rise in [Ca²⁺]_i following application of methacholine.

Calcium entry RNAi screen

By monitoring agonist- and thapsigargin-induced CCE in HEK293 cells, an RNAi screen was performed using SMARTpool siRNA reagents (Dharmacon). The screen involved transfection with SMARTpool siRNA, a mixture of four siRNAs designed and synthesized by the vendor. In preparation for siRNA knockdown, HEK293 cells were plated in a 96-well plate (at 10 000 cells per well) (day 1). On day 2, cells were transfected with various siRNAs using metafectene, and including siGLO (Dharmacon) as a marker. After 6 h incubation, the medium bathing the cells was replaced by complete DMEM. Transfected cells were maintained in culture until day 4 when [Ca²⁺]_i measurements were performed.

The effects of siRNA on Ca²⁺ entry were screened using cells seeded in 96-well plates and a fluorescence imaging plate reader (FLIPR384, Molecular Devices, CA, USA). Agonist- and thapsigargin-induced [Ca²⁺]_i changes were measured in transfected HEK293 cells grown on a 96-well plate and loaded with a calcium-sensitive ‘no wash’ dye (FLIPR Calcium 3 Assay kit, Molecular Devices Corp., Sunnyvale, CA, USA). Fluorescence was monitored on a fluorescence imaging plate reader, FLIPR384, by exciting the calcium indicator at 488 nm, and collecting emission fluorescence at 510–570 nm.

Materials

Methacholine was purchased from Sigma (St Louis, MO, USA), thapsigargin from Alexis (San Diego, CA, USA), fura-5F/AM from Molecular Probes (Eugene, OR, USA) and arachidonic acid, (5,8,11,14-eicosatetraenoic acid) was obtained from BioMol (PA, USA). SMARTpool combinations of four siRNAs against Stim1, Stim2 and 34 TRP genes, as well as a control siRNA (siCONTROL) and fluorescent siRNA (siGLO) were obtained from Dharmacon.

	Results and Discussion

Top Abstract Introduction Methods Results and Discussion References

 
Figure 1 demonstrates the effectiveness of functional knockdown of capacitative calcium entry in HEK293 cells utilizing an siRNA duplex as previously described (Mercer et al. 2006). As shown in Fig. 1, treatment of HEK293 cells with Stim1 siRNA reduced the thapsigargin-induced calcium entry with no effect on the depletion of intracellular calcium by thapsigargin (Fig. 1C). It is interesting that the lack of effect on the size of the thapsigargin-sensitive pool indicates that HEK293 cells can maintain normal endoplasmic reticulum Ca²⁺ stores with reduced (but not zero) store-operated Ca²⁺ entry. Treatment of cells with the siRNA constructs siGLO or siCONTROL had no effect on either thapsigargin-induced calcium release or calcium entry (Fig. 1A and B, respectively). Data summarizing the effects of each condition on the peak calcium release and peak calcium entry are shown in Fig. 1D.

View larger version (21K): [in this window] [in a new window]   Figure 1.  Effects of RNAi knockdown on thapsigargin-induced calcium responses in HEK293 cells using single-cell analysis HEK293 cells transfected with siGLO (A), siCONTROL (B) or Stim1 siRNA (C) were loaded with fura-5F and treated with thapsigargin (TG) in the absence and then the presence of 1.8 mM extracellular Ca2+. For each condition, data are summarized in D for the amplitude of thapsigargin-induced Ca2+ release in the absence of extracellular Ca2+ (TG-release), and the amplitude of thapsigargin-induced Ca2+ entry in the presence of extracellular Ca2+ (Peak entry). The data are means ± S.E.M. for six (A and B) and three (C) independent experiments.

View larger version (21K): [in this window] [in a new window]   Figure 2.  Thapsigargin-induced calcium entry can be rescued by over expression of Stim1 As described in the Methods, HEK293 cells transfected with Stim1 siRNA (A and C) and siGLO (B), were subsequently transfected with cDNA for EYFP (A and B) or EYFP–Stim1 (C). The cells were then loaded with fura-5F and treated with thapsigargin (TG) in the absence and then the presence of 1.8 mM extracellular Ca2+. For each condition, data are summarized in D for the amplitude of thapsigargin-induced Ca2+ release in the absence of extracellular Ca2+ (TG-release), and the amplitude of thapsigargin-induced Ca2+ entry in the presence of extracellular Ca2+ (Peak entry). The data are means ± S.E.M. for three independent experiments.

View larger version (21K): [in this window] [in a new window]   Figure 3.  Thapsigargin (TG) induced calcium entry cannot be rescued by over expression of Stim2 As described in the Methods, HEK293 cells transfected with siGLO (A) or Stim1 siRNA (B and C), were subsequently transfected with cDNA for EYFP (A and B) or Stim2 together with EYFP (C). The cells were then loaded with fura-5F and treated with thapsigargin (TG) in the absence and then the presence of 1.8 mM extracellular Ca2+. For each condition, data are summarized in D for the amplitude of thapsigargin-induced Ca2+ release in the absence of extracellular Ca2+ (TG-release), and the amplitude of thapsigargin-induced Ca2+ entry in the presence of extracellular Ca2+ (Peak entry). The data are means ± S.E.M. for three independent experiments. Analysis of variance followed by Tukey-Kramer multiple comparisons test revealed that for entry, siGLO was significantly greater than either Stim1 siRNA or Stim1 siRNA + Stim2 siRNA (P < 0.01), but the latter were not significantly different from one another (P > 0.05).

Thus far, we have confirmed findings from other laboratories that knocking down the levels of Stim1 in HEK293 cells substantially reduces the calcium entry signal activated under maximal stimulus conditions with thapsigargin, and by use of a rescue protocol, demonstrated that the effects of the specific siRNA duplex we have employed are specific for effects on Stim1 expression. However, the relevance of findings with complete Ca²⁺ store depletion by thapsigargin to signalling mechanisms with agonists at physiological stimulation strengths has been rightly questioned (Shuttleworth, 1999; van Rossum et al. 2004). Thus, we next wanted to use these same molecular approaches to investigate the Ca²⁺ entry pathway underlying a more physiologically relevant [Ca²⁺]_i response (i.e. agonist-induced [Ca²⁺]_i oscillations).

In experiments summarized in Fig. 4, we have characterized the effect of Stim1 siRNA on methacholine-induced calcium oscillations in HEK293 cells. As previously reported (Bird & Putney, 2005), treatment of fura-5F-loaded HEK293 cells with 5 µM methacholine in the presence of 1.8 mM extracellular Ca²⁺ results in a sustained, or slowly declining oscillatory Ca²⁺ response in the majority of cells (Fig. 4A). As previously described (Bird & Putney, 2005), the characteristics of this Ca²⁺ response to 5 µM methacholine are that 80% of the cells respond (Fig. 4D) and with an oscillatory response that is sustained over the 25 min period tested (Fig. 4E). By contrast, the oscillations in HEK293 cells treated with Stim1 siRNA in response to 5 µM methacholine were not sustained but transient (Fig. 4B and E). This would be consistent with the loss of a calcium entry required to support oscillations. However, as indicated in Fig. 4D, the percentage of responding cells under these conditions falls to 45%. These two characteristics, reduced responsiveness and transient oscillatory frequency, bare the hallmarks of an agonist response observed with a lower agonist concentration (see data for response to 1 µM methacholine by Bird & Putney (2005)).

View larger version (37K): [in this window] [in a new window]   Figure 4.  Effect of Stim1 RNAi knockdown on oscillatory calcium response in HEK293 cells HEK293 cells transfected with siGLO (A) or Stim1 siRNA (B and C) were loaded with fura-5F and treated with low concentrations of methacholine (MeCh) in Hepes-buffered salt solution containing 1.8 mM extracellular calcium. A, typical calcium response of a field of siGLO-transfected HEK293 cells to 5 µM MeCh. B and C, typical calcium response of a field of Stim1 siRNA transfected HEK293 cells to 5 and 10 µM MeCh, respectively. D, summarized data of the percentage responding cells observed in A–C (means ± S.E.M. for four independent experiments). E, summarized calcium oscillation frequency data of the effects of 5 µM () MeCh (added at arrow) in siGLO transfected HEK293 cells, and 5 µM () and 10 µM () MeCh (added at arrow) in Stim1 siRNA transfected HEK293. The data are means ± S.E.M. of four independent experiments.

The above findings are consistent with a primary effect of Stim1 knockdown on loss of calcium entry necessary for sustained oscillations. To further evaluate this interpretation, we investigated the effects of ‘Gd³⁺ insulation’ on the oscillatory calcium response in Stim1 knockdown cells. That is, we incubated HEK293 cells in the presence of a high concentration of GdCl₃ (1 mM), such that transmembrane calcium fluxes across the plasma membrane in both directions were prevented. Under these conditions, HEK293 cells do not lose intracellular calcium to the extracellular space, and agonist-induced [Ca²⁺]_i oscillations are sustained despite the absence of calcium entry (Sneyd et al. 2004; Bird & Putney, 2005). As shown in Fig. 5A, the transient oscillatory calcium response observed with 10 µM methacholine in Stim1 siRNA-treated cells is now sustained in the presence of 1 mM GdCl₃. Thus, the primary effect of Stim1 knockdown on agonist-induced [Ca²⁺]_i oscillations is to cause loss of the calcium entry necessary to sustain them. As a final proof of this idea, we transiently expressed Stim1 in these same Stim1 siRNA-treated cells with the result that the sustained oscillatory calcium response to methacholine was now rescued (Fig. 5B).

View larger version (23K): [in this window] [in a new window]   Figure 5.  A sustained oscillatory calcium response to methacholine (MeCh) can be maintained in Stim1 siRNA-treated HEK293 cells by ‘Gd3+ insulation’ or by expression of Stim1 A, HEK293 cells transfected with siGLO () or Stim1 siRNA (,) were loaded with fura-5F and treated with low concentrations of MeCh in Hepes-buffered salt solution (HBSS) containing 1.8 mM extracellular calcium. The summarized calcium oscillation frequency data are shown for siGLO-transfected cells responding to 5 µM MeCh () and Stim1 siRNA transfected cells responding to 10 µM MeCh (,) (MeCh added at arrow). For one set of Stim1 siRNA treated cells (), the cells were treated under ‘Gd3+ insulation’ conditions (nominally calcium free HBSS supplemented with 1 mM GdCl3). The data are means ± S.E.M. of four independent experiments. B, HEK293 cells transfected with Stim1 siRNA were subsequently transfected with cDNA for enhanced yellow fluorescent protein (EYFP; ) or EYFP–Stim1 (). Fura-5F-loaded cells were then treated with 20 µM MeCh in HBSS containing 1.8 mM extracellular calcium. The summarized calcium oscillation frequency data for the MeCh response (added at arrow) are shown. The data are means ± S.E.M. of three independent experiments.

View larger version (29K): [in this window] [in a new window]   Figure 6.  RNAi knockdown of Stim1 does not prevent a sustained oscillatory response to methacholine in the presence of TRPC3, nor does it reduce the calcium entry response to arachidonic acid A, HEK293 cells transfected with siGLO () or Stim1 siRNA () were loaded with fura-5F and treated with low concentrations of methacholine (MeCh) in Hepes-buffered salt solution (HBSS) containing 1.8 mM extracellular calcium. The summarized calcium oscillation frequency data are shown for siGLO-transfected cells responding to 5 µM MeCh () and Stim1 siRNA transfected cells responding to 10 µM MeCh () (MeCh added at arrow). After a 25 min MeCh response period in siGLO-transfected cells, 5 µM Gd3+ was added to demonstrate that the oscillatory response could be blocked. The data are means ± S.E.M. of three independent experiments. B, in parallel with the experiments performed in A, HEK293 cells stably expressing HTRPC3 (HEK–TRCPC3 cells) were transfected with Stim1 siRNA, loaded with fura-5F and treated with 10 µM MeCh in HBSS containing 5 µM GdCl3 (to block store-operated calcium entry) and with either 1.8 mM extracellular calcium present (), or nominally calcium free (). The summarized calcium oscillation frequency data are shown. The data are means ± S.E.M. of three independent experiments. C, as described in the Methods, HEK293 cells transfected with siGLO Orai1 siRNA or Stim1 siRNA were plated at low density, loaded with fura-5F and treated with 30 µM arachidonic acid (AA). The initial treatment with AA was in nominally calcium-free HBSS, and then 1.8 mM extracellular calcium was added back to monitor calcium entry. The data presented are means from three independent experiments. D, summarized data for AA-induced calcium entry ( ratio) shown in C. Data are means ± SEM for three independent experiments.

Effects of knockdown of Orai1 and TRP channels

In addition to Stim1, capacitative or store-operated Ca²⁺ entry depends upon a plasma membrane protein, Orai1 (Feske et al. 2006; Zhang et al. 2006; Vig et al. 2006b). Recent findings indicate that Orai1 probably functions as a subunit of the store-operated channel itself (Yeromin et al. 2006; Prakriya et al. 2006; Vig et al. 2006a). As shown in Fig. 7, knockdown of Orai1 by RNAi (Mercer et al. 2006) significantly reduced the frequency of [Ca²⁺]_i oscillations without reducing the percentage of cells responding to methacholine (Fig. 7A and B). This effect was due to reduction of Ca²⁺ entry because the inhibition resulting from knockdown of Orai1 was prevented by use of 1 mM Gd³⁺ to prevent both Ca²⁺ entry and Ca²⁺ extrusion (Fig. 7C). Finally, similar to the findings for Stim1, knockdown of Orai1 by RNAi did not affect arachidonic acid-induced Ca²⁺ entry (Fig. 6C).

View larger version (15K): [in this window] [in a new window]   Figure 7.  Effect of Orai1 RNAi knockdown on oscillatory calcium response in HEK293 cells HEK293 cells transfected with siGLO or Orai1 siRNA were loaded with fura-5F and treated with low concentrations of methacholine (MeCh) in Hepes-buffered salt solution (HBSS) containing 1.8 mM extracellular calcium. A, summarized data of the percentage cells responding to 5 µM MeCh (means ± S.E.M. for four independent experiments). B, summarized calcium oscillation frequency data on the effects of 5 µM () MeCh (added at arrow) in siGLO-transfected HEK293 cells, and 5 µM () MeCh (added at arrow) in Orai1 siRNA-transfected HEK293. The data are means ± S.E.M. of four independent experiments. C, summarized calcium oscillation frequency data are shown for siGLO- () and Orai1 siRNA (,)-transfected cells responding to 5 µM methacholine (MeCh added at arrow). For one set of Orai1 siRNA-treated cells (), the cells were treated under ‘Gd3+ insulation’ conditions (nominally calcium free HBSS supplemented with 1 mM GdCl3). The data are means ± S.E.M. of three independent experiments.

View larger version (32K): [in this window] [in a new window]   Figure 8.  Characterization of the effects of RNAi knockdown on [Ca2+]i signalling responses to methacholine in HEK293 cells HEK293 cells grown in a 96-well well plate were transfected with siRNA against various targets (see Table 1), including Stim1 and Stim2. [Ca2+]i responses to 300 µM methacholine (MeCh) or 2 µM thapsigargin (TG) were monitored in the presence of 1.8 mM extracellular Ca2+ (A and C), or after addition of 5 µM Gd3+ to block Ca2+ entry (B and D). The data presented are from a single experiment, with each trace the average response from two wells. The data are representative of three similar experiments.

Together, our data confirm recent studies implicating Stim1, a Ca²⁺-binding protein located in the membrane of the endoplasmic reticulum, as a potential Ca²⁺ sensor that initiates the process of store-operated Ca²⁺ entry. In contrast with the findings of Liou et al. (2005) and consistent with the findings of Roos et al. (2005), we found no evidence of such a role for Stim2; knockdown of Stim2 had no effect on Ca²⁺ signalling in HEK293 cells and, more significantly, Stim2 could not rescue [Ca²⁺]_i responses after knockdown of Stim1. We have provided the first demonstration of an essential role for Stim1 and also Orai1 in the mechanism of [Ca²⁺]_i signalling with physiological stimulus strength, when [Ca²⁺]_i levels undergo oscillations. We have also demonstrated for the first time that TRPC3 channels and arachidonic acid-activated channels, both of which are activated downstream of phospholipase C, but not by Ca²⁺-store depletion, do not require Stim1.

While this work was under review, a publication appeared by Mignen et al. (2007) proposing a role for Stim1 in arachidonic acid-activated current in HEK293 cells. In contrast to the findings reported here, these authors reported suppression of arachidonate-activated currents by knockdown of Stim1. The reason for these discrepant results is not clear. However, interestingly, Mignen et al. (2007) also reported that it was specifically Stim1 in the plasma membrane that supported the arachidonate-activated currents. In our experiments in which we rescued [Ca²⁺]_i oscillations following Stim1 knockdown, we utilized an EYFP–Stim1 construct which we (Mercer et al. 2006) and others (Liou et al. 2005) have shown does not traffic to the plasma membrane. Thus, it is unlikely that the role of Stim1 shown in our current study involves regulation of arachidonic acid-activated currents.

The current findings extend and support our previous conclusions on the nature of the Ca²⁺ entry mechanism that supports [Ca²⁺]_i oscillations in this model system. Previously, we reported that it was predominantly if not exclusively the store-operated pathway that supported [Ca²⁺]_i oscillations. That argument was based primarily on pharmacological properties of the store-operated pathway. Here we utilized a molecular criterion – the requirement for Stim1 and Orai1 – to further support this conclusion. The conclusion drawn by Yan et al. (2006) regarding the lack of a role for store-operated entry in C. elegans intestinal cell oscillations may be specific for that particular cell type, and at present should probably not be assumed to be a general property. The current study supports our previous conclusion (Bird & Putney, 2005) that [Ca²⁺]_i oscillations are dependent on store-operated channels in the HEK293 cell model, but our results with TRPC3 over-expressing cells show that [Ca²⁺]_i oscillations can be supported by non-store-operated channel mechanisms. Thus, we believe it is important to consider the question of the nature of regulated Ca²⁺ entry responsible for [Ca²⁺]_i oscillations or other important response patterns independently for each particular cell type, receptor type or physiological process. The advent of molecular players in the store-operated pathway provides powerful tools for such investigations. With these and other tools, we can continue to probe the molecular and cellular processes that integrate plasma membrane Ca²⁺ fluxes with the complex [Ca²⁺]_i oscillations.

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	Acknowledgements

 
We thank Drs Elizabeth Murphy and Dave Miller who read the manuscript and provided helpful comments. This work was supported by funds from the Intramural Program of the National Institute of Environmental Health Science (NIEHS) and NIH.

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