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NEUROSCIENCE |
1 Department of Pharmacology, Southern Illinois University School of Medicine, Springfield, IL 62702, USA
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Abstract |
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37°C) PKC-mediated enhancement of mEPSC frequency is significantly decreased by a specific TRPV1 antagonist, suggesting a physiological role of TRPV1 at the central terminals. Furthermore, bradykinin (BK) significantly potentiated TRPV1-modulated synaptic responses by activating the PLC-PKC pathway. Our results indicate that TRPV1 activation can modulate excitatory synaptic transmission at the first sensory synapse and its effects can further be augmented by activation of PKC. Increased gain of sensory input by TRPV1-induced enhancement of glutamate release and its potentiation by various inflammatory mediators may contribute to persistent pain conditions. Selective targeting of TRPV1 expressed on the central terminals of sensory neurons may serve as a strategy to alleviate chronic intractable pain conditions.
(Received 20 January 2007;
accepted after revision 8 March 2007;
first published online 15 March 2007)
Corresponding author L. S. Premkumar: Department of Pharmacology, Southern Illinois University School of Medicine, Springfield, IL 62702, USA. Email: lpremkumar{at}siumed.edu
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Introduction |
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The transient receptor potential vanilloid 1 (TRPV1) is a non-selective, calcium-permeable cation channel that is expressed on both the central and peripheral terminals of small to medium diameter sensory neurons (Caterina et al. 1997; Tominaga et al. 1998; Nakatsuka et al. 2002; Baccei et al. 2003). TRPV1 is gated by protons, heat, lipid mediators such as anandamide, N-arachidonoyl-dopamine (NADA), metabolites of arachidonic acid (Zygmunt et al. 1999; Hwang et al. 2000; Huang et al. 2002; De Petrocellis et al. 2004), vanilloid compounds such as capsaicin, the pungent ingredient in hot chilli peppers (Caterina et al. 1997), and resiniferatoxin (RTX), an ultra-potent agonist obtained from a succulent plant, Euphorbia resinifera (Szallasi & Blumberg, 1999; Raisinghani et al. 2005).
TRPV1 is widely implicated in various inflammatory and neuropathic pain conditions (Holzer, 2004). Studies using TRPV1 knock-out mice suggest that it is critical in mediating inflammatory thermal hyperalgesia (Caterina et al. 2000; Davis et al. 2000). Recently, selective ablation of TRPV1-expressing DRG neuronal cell bodies has been reported to be beneficial in treating pain in canine models of bone cancer and arthritis (Karai et al. 2004; Brown et al. 2005). These studies underscore the importance of TRPV1 in mediating various thermal inflammatory and chronic pain conditions.
Phosphorylation by protein kinase A (PKA) and protein kinase C (PKC) is known to modulate the properties of TRPV1 (Caterina & Julius, 2001; Holzer, 2004). Continuous agonist application results in dramatic calcium-dependent run-down of TRPV1-mediated currents, designated as acute desensitization. On the other hand, a calcium-dependent decrease in TRPV1 responses with repeated agonist applications is referred to as tachyphylaxis. Interestingly, PKA-mediated phosphorylation of TRPV1 prevents tachyphylaxis (Bhave et al. 2002; Mohapatra & Nau, 2003), whereas PKC-mediated phosphorylation is known to potentiate heat-, protons- and agonist-activated TRPV1 currents (Cesare & McNaughton, 1996; Premkumar & Ahern, 2000; Vellani et al. 2001; Tominaga et al. 2001; Crandall et al. 2002; Premkumar et al. 2004). Importantly, PKC-activating phorbol esters and the inflammatory mediator bradykinin (BK) have been shown to cause dose-dependent lowering of the thermal activation threshold of TRPV1 to below body temperature (Numazaki et al. 2002; Sugiura et al. 2002). Furthermore, studies in mice lacking different isoforms of PKC have established the pro-nociceptive role of PKC in the spinal cord. PKC-
knock-out mice failed to develop neuropathic pain syndromes following partial sciatic nerve ligation but demonstrated normal responses to acute nociceptive stimuli (Malmberg et al. 1997). In addition, PKC-
knock-out mice did not develop thermal or mechanical hyperalgesia mediated by administration of epinephrine or acetic acid (Khasar et al. 1999).
BK is released at the site of tissue injury or in response to inflammation when protease kallikreins cleave the precursor kininogens (Couture et al. 2001). The BK receptor, B2, is constitutively expressed in both primary afferent sensory neurons and spinal cord neurons (Seabrook et al. 1997; Wang et al. 2005). The role of BK in sensitizing nociceptors in the periphery is well established (Dray et al. 1988; Couture et al. 2001). However, a recent study suggests that BK may enhance glutamatergic synaptic transmission between spinal cord neurons (Wang et al. 2005). BK has been reported to sensitize TRPV1 in dissociated DRG neuronal cultures via second messengers produced downstream to B2 receptor activation (Shin et al. 2002), mainly by relieving the inhibitory effect of phosphatidylinositol-4,5-bisphosphate (PIP2) (Chuang et al. 2001) and by activating protein kinase C (Cesare & McNaughton, 1996; Premkumar & Ahern, 2000; Sugiura et al. 2002). However, whether BK can influence TRPV1-modulated synaptic transmission is still unclear. In the present study, we have examined the role of TRPV1 in modulating glutamatergic synaptic transmission at the first sensory synapse. Our results demonstrate for the first time that phorbol esters and BK by activating PKC potentiate excitatory synaptic transmission at the first sensory synapse by sensitizing TRPV1. Significantly, at normal body temperatures, phosphorylated TRPV1 is active and influences synaptic transmission. Some of these data have appeared in an abstract form elsewhere (Sikand & Premkumar, 2005).
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Methods |
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All animal experiment protocols used in this study were approved by the Southern Illinois University School of Medicine Animal Care Committee. The animals were cared for according to the standards recommended by the National Institutes of Health (NIH). Pregnant Sprague–Dawley rats were heavily anaesthetized with a lethal dose of Nembutal (80 mg kg–1, I.P.), and E18 embryos were removed. The embryos were decapitated under hypothermic anaesthesia. DRG and spinal cord DH were dissected and collected in L-15 medium (Invitrogen, Carlsbad, CA, USA) under sterile conditions. The DRG and DH tissue were then triturated to dissociate the neurons in Neurobasal medium (Invitrogen) containing pencillin (50 units ml–1) and streptomycin (25 µg ml–1). The neurons were plated on glass coverslips previously coated with poly D-lysine (10 µg ml–1, Sigma, St Louis, MO). In some dishes, only DH or DRG neurons were plated to obtain DH or DRG neuronal monocultures. Neurons were maintained in Neurobasal culture medium (Invitrogen) that also contained nerve growth factor (2.5S NGF; 100 ng ml–1; Roche Molecular Biochemicals, Indianapolis, IN, USA), 0.5 mM L-glutamine (Invitrogen) and B27 (a serum free supplement) (Invitrogen). The cultures were maintained at 37°C in a humidified atmosphere of 95% air and 5% CO2 and the culture medium was replaced weekly.
Patch-clamp recordings from DH neurons in DRG–DH cocultures
Cover slips with the cocultured neurons were placed in a recording chamber and mounted on the stage of an Olympus IMT-2 microscope (Olympus, Lake Success, NY, USA). The DH neurons were distinguished from the DRG neurons based on their morphology and electrophysiological properties. The DH neurons selected were fusiform, pyramidal or multipolar in shape in contrast to the rounded pseudounipolar characteristics of the DRG neurons. Moreover, in our cultures no mEPSCs could be recorded when a DRG neuron was voltage clamped and capsaicin application resulted in an inward whole cell current. However, mEPSCs in control and upon capsaicin application could be recorded when a DH neuron was voltage clamped. The bath solution contained (mM): 150 NaCl; 5 KCl; 2 MgCl2; 0.1 CaCl2; 10 glucose; 10 Hepes; and the pH was adjusted to 7.4 with NaOH. The recording pipettes were made from borosilicate glass (World Precision Instruments, Sarasota, FL, USA), which had the resistance of 3–10 M
and filled with a solution that contained (mM): 140 CsMeSO3; 5 CsCl; 10 Hepes; 5 MgCl2; 10 EGTA; 5 Mg-ATP; 1 Li-GTP; and pH was adjusted to 7.4 with CsOH. All experiments were performed at room temperature (24 ± 2°C) except when effects of PKC-mediated phosphorylation of TRPV1 were studied at 37°C. In order to record fast excitatory postsynaptic currents, DH neurons were voltage clamped (EPC10, HEKA) at –60 mV (close to ECl). The capacitance and the series resistance were compensated and the input resistance of the cell was monitored. The data were filtered at 2.5 kHz and digitized at 5 kHz. Adequate voltage clamp was confirmed by recording the mEPSCs at different voltages and determining their reversal potentials. A new coverslip was used for every experiment to prevent run-down of mEPSCs and the activation of second messenger pathways.
Neurons were perfused with standard bathing solution plus lidocaine (10 mM), strychnine (1 µM) and bicuculline (10 µM). A high concentration of lidocaine was used to block both TTX-sensitive and TTX-resistant Na+ channels. Effects of TRPV1 receptor activation on glutamatergic synaptic transmission were determined by application of capsaicin (10–100 nM). mEPSCs sensitive to block by the AMPA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) were recorded for 2 min in presence of lidocaine, following which, capsaicin was bath-applied for 30 s and changes in mEPSCs were recorded continuously thereafter. Effects of PDBu (1 µM) and BK (1 µM) were tested following wash of capsaicin for 4–5 min to allow increases in mEPSCs to return to the basal control conditions. Following a 2 min pretreatment with PDBu or BK, capsaicin was applied and changes in mEPSC frequency and amplitude were recorded. In experiments measuring mEPSCs in the absence of extracellular Ca2+, the bath solution was the same as the standard solution except that Ca2+ was omitted and 5 mM EGTA was added.
The experiments requiring higher temperature were performed by flowing solutions through a heated coil (TC-324B, Warner Instrument Corp., Hamden, CT, USA). The final temperature of the recording chamber was maintained between 36 and 38°C. The mEPSC events were recorded from voltage clamped DH neurons under these conditions as described above.
Data analysis
Data were analysed using Mini Analysis Program (Synaptosoft, Decatur, GA, USA). The amplitude and frequency of the synaptic events were determined from 20 s data segments to avoid desensitization of responses over time. Only mEPSCs with amplitudes 5 pA or greater were analysed.
All the chemicals used in this study were obtained from Sigma (St Louis, MO, USA). The working concentrations of capsaicin, BK, U73122, U73343, PDBu, BIM and capsazepine were prepared fresh before the experiments from either an ethanol or DMSO stock. N-(4-Tertiarybutylphenyl)-4-(3-chloropyridin-2-yl)tetr-ahydropyrazine-1(2H)-carbox-amide (BCTC) was a gift from a pharmaceutical company and was prepared fresh from a DMSO stock. The maximum concentration of ethanol and DMSO were < 0.01 and 0.02%, respectively.
Statistics
Kolmogorov–Smirnov (KS) test was used to compare the cumulative probability curves for interevent intervals and amplitude between various treatment groups. Data are represented as means ± S.E.M. and expressed as a percentage of control, which is scaled to 100%. Student's paired t test was used for statistical comparisons and significance was considered at P < 0.05.
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Results |
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Using DRG–DH neuronal cocultures, mEPSCs were recorded from DH neurons voltage clamped at –60 mV in presence of extracellular solution containing lidocaine (10 mM), bicuculline (10 µM) and strychnine (1 µM) to block tetrodotoxin-sensitive and -resistant sodium channels, GABAA and glycine receptors, respectively. mEPSC events were recorded in the presence of low extracellular calcium (0.1 mM) to prevent rapid run-down of synaptic events following application of capsaicin. The AMPA receptor antagonist CNQX (10 µM) completely abolished all synaptic events, suggesting that they are glutamate mediated (data not shown). Application of capsaicin robustly increased the frequency of mEPSCs in a concentration-dependent manner. Overall, the mEPSC frequency expressed as a percentage of control increased significantly to 664.04 ± 135.2% with 10 nM capsaicin (range 213– 1966%, n = 17, P < 0.05) and 3714.3 ± 927% with 100 nM capsaicin (range 309–8700%, n = 11, P < 0.05) (Fig. 1A, B and D). The mean mEPSC amplitude was not significantly altered (control, 9.5 ± 0.5 pA, n = 17; 10 nM capsaicin, 10.9 ± 0.8 pA, n = 17 and control, 8.1 ± 0.6 pA, n = 11; 100 nM capsaicin, 9.6 ± 1.3 pA, n = 11) (Fig. 1C). An increase in the mEPSC frequency but not the amplitude suggests that capsaicin acts presynaptically to increase glutamate release from the central terminals of sensory neurons. The facilitatory effect of capsaicin on synaptic transmission was observed in a high percentage (> 80%) of voltage clamped DH neurons in DRG–DH neuronal cocultures. The higher incidence of obtaining synaptic capsaicin responses, even though only 30% of DRG neurons express TRPV1, is probably due to multiple synaptic inputs received by a DH neuron from TRPV1-expressing DRG neurons in our coculture system.
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In order to determine whether capsaicin could modulate the inhibitory synaptic transmission mediated by GABAergic and glycinergic interneurons at the first sensory synapse, recordings were made in the presence of the AMPA and NMDA receptor antagonists CNQX (10 µM) and 2-amino-5-phosphonovaleric acid (APV; 20 µM), respectively, at 0 mV, where excitatory currents reverse and inhibitory currents can be recorded in isolation using our experimental conditions. Application of capsaicin (100 nM) failed to enhance the inhibitory transmission in the cocultures (data not shown). These observations suggest that capsaicin selectively enhances excitatory synaptic transmission.
To demonstrate that increase in mEPSCs in response to capsaicin application is due to TRPV1 activation, a TRPV1 selective antagonist, capsazepine (Cpz), was used. Cpz (10 µM) abolished the facilitatory effects of 10 nM capsaicin on mEPSC frequency (Fig. 1D). Capsaicin-induced mEPSC frequency decreased from 797 ± 180% in capsaicin treated cells (n = 5, P < 0.05) to 132 ± 29% in presence of Cpz (n = 5, P < 0.05). There was no change in the amplitude of mEPSCs in the presence of Cpz (9.2 ± 1.2 pA, n = 5), when compared to the control (9.3 ± 1.2 pA, n = 5). These results confirm that capsaicin acts via TRPV1 located on the central terminals of primary afferents to increase glutamate release. In addition, exposure to Cpz alone at room temperature did not affect basal synaptic activity (103.92 ± 20.1%, n = 6), suggesting that there is no tonic TRPV1 activation at this synapse in our coculture system. Further, we determined whether extracellular calcium is necessary for the increase in mEPSC frequency following TRPV1 activation. Recordings were made using a Ca2+-free extracellular solution containing calcium chelator, EGTA (5 mM). Application of capsaicin (100 nM) for 30 s failed to produce a significant increase in the frequency of mEPSCs (105.67 ± 8.87%, n = 3), suggesting that extracellular Ca2+ is essential for the observed effects of capsaicin (Fig. 1D).
Changes in mEPSCs in response to continuous and repeated application of capsaicin
Capsaicin-induced whole-cell currents have been shown to exhibit calcium-dependent desensitization and tachyphylaxis (Caterina & Julius, 2001; Holzer, 2004). Receptor desensitization is an inherent property of ligand-gated channels, which is usually dependent on the time and concentration of agonist exposure, but is calcium independent. In contrast, desensitization of TRPV1 is a calcium-dependent phenomenon. Continued application of capsaicin decreased the frequency of mEPSCs over time (Figs 1A and 2A and B). Change in mEPSC frequency in successive 5 s intervals immediately following capsaicin application was analysed. Capsaicin-mediated increases in mEPSC events showed a gradual decrease with time. The frequency of mEPSCs in successive 5 s intervals following capsaicin (10 nM) application, expressed as a percentage of control, were 581.65 ± 112% (n = 8); 644.45 ± 104% (n = 8); 447.7 ± 58% (n = 8); and 328.5 ± 36.7% (n = 6) (Fig. 2B). The run-down of synaptic events with continuous capsaicin application may be a result of Ca2+-dependent decrease in TRPV1 channel function.
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Enhancement of synaptic transmission by phorbol esters
In order to study the effects of PKC-mediated sensitization of TRPV1 on synaptic transmission, we used the phorbol ester phorbol 12,13-dibutyrate (PDBu). Previous studies have utilized PDBu to activate PKC and examined synaptic transmission in the CNS (Malenka et al. 1986; Muller et al. 1991; Hori et al. 1996; Rhee et al. 2002). In this study, application of PDBu (10 nM to 1 µM) alone induced a dose-dependent increase in the frequency but not the amplitude of mEPSCs at this synapse (Fig. 3A, C and E), suggesting presynaptic enhancement of glutamate release. On an average, mEPSC frequency significantly increased in a dose-dependent manner following PDBu application (10 nM, 267.8 ± 25%, n = 6, P < 0.05; 100 nM, 327.6 ± 65.5%, n = 5, P < 0.01; 1 µM, 400 ± 64%, n = 10, P < 0.001) (Fig. 3E), whereas the mean amplitude remained unaltered (control, 10.3 ± 0.6 pA, n = 6; 10 nM PDBu, 11.9 ± 1.8 pA, n = 6; control, 11.5 ± 1.1 pA, n = 5; 100 nM PDBu, 10 ± 0.9 pA, n = 5; and control, 13.5 ± 2.8 pA, n = 10; 1 µM PDBu, 13.4 ± 1.3 (n = 10). PDBu-induced enhancement of synaptic activity was readily reversible following a 3–4 min wash; the increases in mEPSCs returned to the basal levels in most recordings (10 nM, 106.4 ± 10.3%, n = 4; 100 nM, 109 ± 11.2%, n = 4; 1 µM, 114 ± 12.4%, n = 4). Moreover, unlike the decrease in the frequency of mEPSCs seen with capsaicin, repeated applications of PDBu (100 nM) increased the mEPSC frequency to a similar extent (first application, 258.6 ± 24.5%, n = 5; second application, 258.2 ± 28%, n = 5; third application, 257.7%, n = 3).
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Potentiation of TRPV1-mediated enhancement of glutamatergic synaptic transmission by PDBu
PKC-mediated phosphorylation robustly potentiates TRPV1 activity and this sensitization plays an important role in chronic pain processing (Cesare & McNaughton, 1996; Premkumar & Ahern, 2000; Chuang et al. 2001; Vellani et al. 2001; Sugiura et al. 2002; Bonnington & McNaughton, 2003). Therefore, we studied the influence of PKC on TRPV1-modulated synaptic transmission at the first sensory synapse. Our earlier experiments have established that the first application of capsaicin resulted in a large increase in synaptic transmission with a significant reduction during second application followed by consistent responses thereafter. Therefore, we used the second response as our control and compared the changes in subsequent responses to determine the effect of PKC on synaptic transmission as a result of TRPV1 phosphorylation. The mEPSC frequency (expressed as percent of control) with second application of capsaicin (100 nM) was 311.7 ± 93.8%, n = 6 and following PDBu pretreatment, the mEPSC frequency with capsaicin increased to 1280.8 ± 236.5%, n = 6, P < 0.05 (Fig. 4A, B and D). The change in frequency of mEPSC with PDBu (1 µM) in these experiments was 318.64 ± 117.5%, n = 6. The average amplitude remained unchanged (control, 9.6 ± 1 pA, n = 6; 100 nM capsaicin, 9.7 ± 1.3 pA, n = 6; 1 µM PDBu, 10.1 ± 1.8 pA, n = 6; capsaicin after PDBu, 10.3 ± 1.6 pA, n = 6). Interestingly, PDBu-mediated direct increase in the synaptic events was seen in all neurons patched even when capsaicin failed to enhance synaptic events. However, further enhancement of capsaicin-mediated synaptic responses following PDBu pretreatment was observed in 66% of capsaicin-sensitive neurons (10 of 15). Of these, 60% of neurons showed profound potentiation and the rest did not demonstrate a statistically significant change with capsaicin after PDBu application. The variability of these responses can be explained by the extent of synaptic connections made to the recording DH neuron. PDBu increases synaptic transmission indiscriminatingly between both DH–DH as well as DRG–DH synapses, whereas capsaicin-mediated increase in mEPSC frequency is specific to TRPV1-expressing DRG and DH neuronal synapses. Thus, the number of synaptic connections between DRG and DH neurons will determine the extent of potentiation observed.
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PKC-mediated phosphorylation has been suggested to sensitize TRPV1 resulting in lowering of its activation threshold from 43°C to below body temperature (Sugiura et al. 2002). We hypothesize that PKC-mediated phosphorylation of TRPV1 expressed in the central terminals of sensory neurons lowers its activation threshold and enhances synaptic transmission. To test this, mEPSC events were recorded at 37°C in the presence of the control solution and after application of PDBu. A 2 min application of PDBu enhanced the synaptic transmission to 227.8 ± 36% of the control, n = 5 (Fig. 6A). Furthermore, to separate the PDBu-mediated direct effects from the TRPV1-modulated component, the selective TRPV1 antagonist N-(4-tertiarybutylphenyl)-4-(3-chloropyridin-2-yl)tetr-ahydropyrazine-1(2H)-carbox-amide (BCTC, 100 nM) was used. Interestingly, the mEPSC frequency decreased significantly in the presence of BCTC to 125.5 ± 5% of control, n = 5, suggesting the involvement of TRPV1 (Fig. 6A, B and D). PDBu and BCTC application did not significantly affect the amplitude of the mEPSC events (Fig. 6C). In addition, application of BCTC alone neither affected the basal synaptic transmission at 37°C nor inhibited PDBu-mediated direct responses at room temperature (data not shown). These findings suggest that PKC-mediated phosphorylation of TRPV1 sensitizes the channel and decreases it activation threshold to body temperature. This mechanism may be important in activating TRPV1 expressed in the central terminals of sensory neurons, in the absence of an agonist or in the presence of low potency endogenous agonists.
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BK is an important inflammatory mediator known to induce thermal hyperalgesia, at least in part by sensitizing TRPV1 (Cesare & McNaughton, 1996; Premkumar & Ahern, 2000; Chuang et al. 2001; Sugiura et al. 2002; Shin et al. 2002). BK, acting via its B2 receptor, activates phospholipase C (PLC) to cleave PIP2 and produce inositol-1,4,5-triphosphate (IP3) and diacylgycerol (DAG) (Burgess et al. 1989; Cesare et al. 1999). DAG can activate PKC directly and IP3 by releasing calcium from internal stores. Furthermore, BK-induced activation of PLC hydrolyses PIP2 to relieve TRPV1 from its tonic inhibition (Chuang et al. 2001). Here, we determined whether BK could induce an increase in TRPV1-modulated excitatory synaptic transmission at the first sensory synapse.
Application of BK (1 µM) produced a direct increase in glutamatergic synaptic transmission. The frequency of mEPSCs increased to 238.4 ± 54% of the control, n = 8. The amplitude of the mEPSCs showed variability and was not statistically significant (control, 10.7 ± 0.9 pA, n = 8; BK, 12.2 ± 1.2 pA, n = 8). The change in the frequency of mEPSCs suggests presynaptic actions of BK, due to activation of BK receptors expressed on the sensory neurons (see also Wang et al. 2005).
To study the influence of BK on TRPV1-modulated synaptic transmission, we determined the effect of BK pretreatment on capsaicin-induced increase in mEPSCs. BK (1 µM) resulted in the potentiation of capsaicin response in 63% of neurons responsive to both capsaicin and BK (5 of 8). The frequency of mEPSC events in response to 10 nM capsaicin increased from 264 ± 55%, n = 5 to 890 ± 83%, n = 5 after 2 min BK application (Fig. 7A, B and D). In these patches, the direct increases mediated by BK were 202.5 ± 27%, n = 5. There was no change in the amplitude of mEPSCs with capsaicin application following BK. The amplitude of mEPSCs with application of capsaicin following BK was 12.1 ± 1.3 pA, n = 5, as compared to capsaicin alone, 11.7 ± 1.5 pA, n = 5 (Fig. 7C). The extent of increase in capsaicin-induced mEPSC frequency mediated by PDBu and BK is variable. As mentioned previously, the variability of these responses can be explained by the extent and unpredictable synaptic connections made to the recording DH neuron. In most experiments, a synergistic response was seen, however, in a few experiments the potentiation was not statistically significant.
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To characterize signalling pathways involved in BK-induced potentiation of TRPV1-modulated synaptic transmission, we examined specific pathways known to be activated by BK (Burgess et al. 1989). To establish the role of PKC-mediated phosphorylation of TRPV1 following BK application, we used the PKC inhibitor BIM in our experiments. Pretreatment with BIM (500 nM for 30 min) inhibited BK-mediated potentiation of TRPV1-modulated synaptic transmission. The mEPSC frequency (expressed as a percentage of control) with the second application of capsaicin were 193.3 ± 33.4%, n = 4, and 133.7 ± 24%, n = 4, with capsaicin after BIM + BK treatment, respectively (Fig. 8A, C and E). These effects are significantly lower when compared to the frequency of mEPSC events with capsaicin after BK (890 ± 83%, n = 5, P < 0.05). The amplitude in these recordings remained unaltered (control, 12.5 ± 2.6 pA, n = 4; capsaicin, 11.8 ± 1.6 pA, n = 4, BK + BIM, 11.7 ± 1.6 pA, n = 4; and capsaicin after BK + BIM, 10.34 ± 0.5 pA, n = 4) (Fig. 8D). These experiments suggest that BK sensitizes TRPV1-modulated synaptic transmission by activating PKC.
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Discussion |
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Enhancement of glutamatergic synaptic transmission by activation of TRPV1 at the first sensory synapse
In this study, we have shown that application of capsaicin robustly enhances excitatory synaptic transmission at the first sensory synapse using DRG–DH neuronal cocultures. In the coculture system it may not be possible to differentiate synapses formed between DRG neurons and DH neurons of specific laminae. However, similar augmentation of synaptic transmission has been observed with capsaicin in the substania gelatinosa (Yang et al. 1998; Nakatsuka et al. 2002; Baccei et al. 2003), hypothalamus (Sasamura et al. 1998), locus coeruleus (Marinelli et al. 2002), nucleus tractus solitarii (Doyle et al. 2002) and substantia nigra (Marinelli et al. 2003) with no effect on GABA- or glycine-mediated synaptic transmission (Yang et al. 1998; Marinelli et al. 2003). However, a recent study has demonstrated that capsaicin enhanced both glutamatergic and GABAergic transmission in the medial preoptic nucleus of the hypothalamus (Karlsson et al. 2005).
The mEPSC frequency increased without affecting the amplitude, suggesting enhanced glutamate release from the terminals of primary afferents upon TRPV1 activation. The TRPV1 antagonist capsazepine by itself did not affect the basal synaptic activity at room temperature, suggesting a lack of tonic TRPV1 activation by endogenous ligands in our DRG–DH neuronal coculture system. However, capsazepine inhibited capsaicin-modulated transmission, suggesting that the effect is specific to TRPV1 activation. Baccei et al. (2003) have demonstrated that capsaicin-induced facilitation of excitatory synaptic transmission in spinal cord slices occurs in the absence of extracellular calcium. Interestingly, in our study, capsaicin-induced increase in mEPSC frequency was dependent on extracellular calcium. This discrepancy could be attributed to a high concentration of capsaicin (2 µM) used in their study. Higher concentrations of capsaicin have been shown to mediate non-specific effects independent of TRPV1 activation (Kawasaki et al. 2004).
Changes in mEPSC frequency with continued and repeated activation of TRPV1
TRPV1-mediated whole cell currents show profound calcium-dependent run-down upon continuous and repeated applications of capsaicin (Caterina & Julius, 2001; Holzer, 2004). Phosphorylation at Ser-116 in the amino-terminus of TRPV1 by PKA is important in inhibiting capsaicin-mediated receptor desensitization (Bhave et al. 2002; Mohapatra & Nau, 2003). Thr-144, Thr-370 and Ser-502 play a crucial role in PKA-mediated sensitization of TRPV1 (Mohapatra & Nau, 2003). In addition, Ca2+–calmodulin-dependent protein kinase II (CaMKII)-mediated phosphorylation of TRPV1 is crucial for its activation by capsaicin. In contrast, activation of calcium-dependent phosphatase calcineurin mediates dephosphorylation of the receptor resulting in its desensitization (Docherty et al. 1996; Jung et al. 2004). Thus, CaMKII and calcineurin may control the subtle balance between the phosphorylated and dephosphorylated states of TRPV1 resulting in either its activation or desensitization (Jung et al. 2004; Rosenbaum et al. 2004). The desensitization observed with TRPV1 is calcium dependent, unlike conventional desensitization observed with ligand-gated channels such as AMPA and nicotinic acetylcholine receptors, which is calcium independent, but dependent on the duration of activation and the concentration of agonist.
Change in the mEPSC frequency following capsaicin application is dependent on the influx of calcium through TRPV1. Therefore, an alteration in mEPSC frequency is an indirect measure of TRPV1 function. We have found that both continued and repeated applications of capsaicin decreased the frequency of mEPSCs. The decrease in mEPSC frequency following capsaicin application was long lasting and did not recover upon washout within the time course of our experiments (60 min). This decrease in transmission upon capsaicin application could be as a result of either Ca2+-dependent receptor desensitization or primary afferent depolarization (PAD), as a result of TRPV1 activation in the presynaptic terminals of sensory neurons. PAD is considered important in limiting firing of sensory neurons upon repeated capsaicin applications (Yang et al. 1998; Doyle et al. 2002; Nakatsuka et al. 2002; Baccei et al. 2003). This involves inactivation of sodium channels due to a relatively depolarized membrane potential secondary to activation of TRPV1. However, whether PAD could affect action potential-independent miniature synaptic events is still unclear. Moreover, this may not be applicable to our study because mEPSCs were recorded in conditions (10 mM lidocaine) that block voltage-gated Na+ and Ca2+ channels. Furthermore, in current-clamp experiments, capsaicin applications resulted in membrane depolarization that was readily reversible. Repeated capsaicin application in these experiments was accompanied by a progressive decrease in response only in the presence of Ca2+, suggesting Ca2+-dependent receptor desensitization (our unpublished data). Therefore, progressive decrease in the synaptic transmission upon either repeated or continuous application of capsaicin is most likely due to Ca2+-dependent desensitization of TRPV1.
Enhancement of synaptic transmission by sensitization of TRPV1 by PKC
Capsaicin-mediated whole-cell currents are robustly potentiated by PKC-activating phorbol esters (PDBu, PMA) in oocytes, in HEK cells heterologously expressing TRPV1 and in native DRG neurons (Premkumar & Ahern, 2000; Vellani et al. 2001; Crandall et al. 2002; Bonnington & McNaughton, 2003). Two serine residues (Ser-502 and Ser-800) on TRPV1 have been identified as being important in PKC-mediated effects (Numazaki et al. 2002). Moreover, inflammatory mediators like BK, prostaglandins (PG), nerve growth factor (NGF) and ATP activate PKC downstream to their G-protein coupled receptors, thereby modulating TRPV1 activity by phosphorylation (Shu & Mendell, 1999; Premkumar & Ahern, 2000; Tominaga et al. 2001; Sugiura et al. 2002). However, the influence of PKC-mediated phosphorylation of TRPV1 in modulating synaptic transmission at the first sensory synapse has not been studied.
An interesting observation in our study is that PDBu mediates direct (PKC-independent) and PKC-dependent effects on synaptic transmission. Activation of PKC has been shown to facilitate excitatory synaptic transmission in the hippocampus (Malenka et al. 1986; Muller et al. 1991; Hori et al. 1996). However, PDBu has also been reported to increase synaptic transmission by itself to a significant extent in hippocampal slices (Rhee et al. 2002). Recent studies have suggested a novel binding site for phorbol ester/DAG on synaptic vesicle priming protein, Munc13–1, which is present only in glutamatergic synapses of the hippocampus (Rhee et al. 2002; Brose & Rosenmund, 2002). Activation of Munc13–1 could explain the direct effects of phorbol esters in increasing synaptic transmission. Recently, some studies have suggested that PDBu may enhance synaptic transmission mainly by enhancing the Ca2+ sensitivity of synaptic vesicle fusion in the calyx of Held (Lou et al. 2005). Thus, PDBu may sensitize the release machinery in the cocultures to enhance synaptic transmission. We have gathered evidence that phosphorylation of TRPV1 plays a role, as the effects we observed with PDBu and capsaicin are synergistic and could be blocked by BIM. However, PDBu-induced direct effect could not be blocked by BIM. These results strongly suggest two different mechanisms for modulation of synaptic transmission by PDBu.
The role of TRPV1 distributed in areas (such as the central terminals of DRG neurons) that are not exposed to temperature ranges required to activate TRPV1 is under debate. Here, we demonstrate that application of PDBu does indeed sensitize TRPV1 and lowers its activation threshold (Numazaki et al. 2002; Sugiura et al. 2002). At 37°C, PKC activation resulted in an increase in mEPSC events, a significant component of which could be blocked by BCTC, a selective TRPV1 antagonist. However, it should be noted that higher concentrations of BCTC may inhibit other TRP channels, for example recently TRPM8 currents have been shown to be blocked by BCTC (Weil et al. 2005; Madrid et al. 2006).
This is an important observation as it further bolsters our hypothesis that persistent TRPV1 activation at body temperature following PKC activation in inflammatory or neuropathic pain maybe an important underlying factor contributing to chronic pain conditions. In addition, the inhibitory affects of BCTC on PDBu-mediated responses were not observed when these experiments were performed at room temperature and TRPV1 antagonism at 37°C in the absence of PDBu did not affect the basal synaptic transmission in the cocultures, suggesting that PKC sensitizes the receptor to activate it at body temperature. Thus, it is possible that multiple physical and chemical stimuli may act synergistically to potently activate TRPV1 and enhance synaptic transmission.
Modulation of synaptic transmission by sensitization of TRPV1 by BK
Inflammatory mediators like BK, PGs, NGF and ATP have been reported to sensitize TRPV1 by activating PKC, PKA and/or CAMKII (Holzer, 2004). We used BK to study its influence on TRPV1-modulated synaptic transmission. Modulation of glutamatergic synaptic transmission by BK has been attributed to activation of its B2 receptors in spinal cord dorsal horn neurons (Wang et al. 2005). We observed that application of BK alone enhanced glutamate release in our cocultures, suggesting a presynaptic locus of action.
BK is known to activate phosholipase C (PLC) resulting in the conversion of PIP2 to IP3 and DAG. DAG directly and IP3 by mobilizing calcium from the internal stores can activate PKC (Burgess et al. 1989). BK also activates phospholipase A2 (PLA2) to produce lipoxygenase metabolites that can activate TRPV1 directly (Hwang et al. 2000; Shin et al. 2002). We have found that BK synergistically increased capsaicin-induced augmentation of mEPSC frequency, which could be blocked by PKC and PLC inhibitors. These data suggest that BK potentiates TRPV1-modulated synaptic transmission by activating the PLC–PKC pathway.
The source and the stimulus for BK release in the spinal cord during inflammation are not fully understood. However, elevated BK levels have been detected in the spinal cord following peripheral noxious insult (Wang et al. 2005). Moreover, administration of BK receptor antagonists has beneficial effects against both acute and chronic pain conditions (Ferreira et al. 2002), further supporting the role of BK in pain modulation. Chronic inflammation and/or pathological conditions of the spinal cord/CNS may increase BK levels in the spinal cord, thereby sensitizing TRPV1 by PKC-mediated phosphorylation. Previous studies have demonstrated that BK-induced sensitization of TRPV1 by PKC reduced its activation threshold to well below body temperature (Sugiura et al. 2002). Thus, as discussed earlier, under these conditions, TRPV1 could be persistently active in the absence of any other agonist to enhance excitatory synaptic transmission. This facilitation of transmission may result in the hyperexcitability of the postsynaptic dorsal horn neurons. Selective targeting of TRPV1 at the central terminals of sensory neurons could be a beneficial therapeutic strategy to treat chronic, refractory pain arising from inaccessible areas such as viscera, bone, etc.
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Bhave G, Zhu W, Wang H, Brasier DJ, Oxford GS & Gereau RW (2002). cAMP-dependent protein kinase regulates desensitization of the capsaicin receptor (VR1) by direct phosphorylation. Neuron 35, 721–731.[CrossRef][Medline]
Bonnington JK & McNaughton PA (2003). Signalling pathways involved in the sensitisation of mouse nociceptive neurones by nerve growth factor. J Physiol 551, 433–446.
Brose N & Rosenmund C (2002). Move over protein kinase C, you've got company: alternative cellular effectors of diacylglycerol and phorbol esters. J Cell Sci 115, 4399–4411.
Brown DC, Iadarola MJ, Perkowski SZ, Erin H, Shofer F, Laszlo KJ, Olah Z & Mannes AJ (2005). Physiologic and antinociceptive effects of intrathecal resiniferatoxin in a canine bone cancer model. Anesthesiology 103, 1052–1059.[CrossRef][Medline]
Burgess GM, Mullaney I, McNeill M, Dunn PM & Rang HP (1989). Second messengers involved in the mechanism of action of bradykinin in sensory neurons in culture. J Neurosci 9, 3314–3325.[Abstract]
Caterina MJ & Julius D (2001). The vanilloid receptor: a molecular gateway to the pain pathway. Annu Rev Neurosci 24, 487–517.[CrossRef][Medline]
Caterina MJ, Leffler A, Malmberg AB, Martin WJ, Trafton J, Petersen-Zeitz KR, Koltzenburg M, Basbaum AI & Julius D (2000). Impaired nociception and pain sensation in mice lacking the capsaicin receptor. Science 288, 306–313.
Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD & Julius D (1997). The capsaicin receptor: a heat-activated ion channel in the pain pathway. Nature 389, 816–824.[CrossRef][Medline]
Cesare P & McNaughton P (1996). A novel heat-activated current in nociceptive neurons and its sensitization by bradykinin. Proc Natl Acad Sci U S A 93, 15435–15439.
Cesare P, Moriondo A, Vellani V & McNaughton PA (1999). Ion channels gated by heat. Proc Natl Acad Sci U S A 96, 7658–7663.
Chuang HH, Prescott ED, Kong H, Shields S, Jordt SE, Basbaum AI, Chao MV & Julius D (2001). Bradykinin and nerve growth factor release the capsaicin receptor from PtdIns(4,5)P2-mediated inhibition. Nature 411, 957–962.[CrossRef][Medline]
Couture R, Harrisson M, Vianna RM & Cloutier F (2001). Kinin receptors in pain and inflammation. Eur J Pharmacol 429, 161–176.[CrossRef][Medline]
Crandall M, Kwash J, Yu W, White G (2002). Activation of protein kinase C sensitizes human VR1 to capsaicin and to moderate decreases in pH at physiological temperatures in Xenopus oocytes. Pain 98, 109–117.[CrossRef][Medline]
Davis JB, Gray J, Gunthorpe MJ, Hatcher JP, Davey PT, Overend P, Harries MH, Latcham J, Clapham C, Atkinson K, Hughes SA, Rance K, Grau E, Harper AJ, Pugh PL, Rogers DC, Bingham S, Randall A & Sheardown SA (2000). Vanilloid receptor-1 is essential for inflammatory thermal hyperalgesia. Nature 405, 183–187.[CrossRef][Medline]
De Petrocellis L, Chu CJ, Moriello AS, Kellner JC, Walker JM & Di Marzo V (2004). Actions of two naturally occurring saturated N-acyldopamines on transient receptor potential vanilloid 1 (TRPV1) channels. Br J Pharmacol 143, 251–256.[CrossRef][Medline]
Docherty RJ, Yeats JC, Bevan S & Boddeke HW (1996). Inhibition of calcineurin inhibits the desensitization of capsaicin-evoked currents in cultured dorsal root ganglion neurones from adult rats. Pflugers Arch 431, 828–837.[Medline]
Doyle MW, Bailey TW, Jin YH & Andresen MC (2002). Vanilloid receptors presynaptically modulate cranial visceral afferent synaptic transmission in nucleus tractus solitarius. J Neurosci 22, 8222–8229.
Dray A, Bettaney J, Forster P & Perkins MN (1988). Bradykinin-induced stimulation of afferent fibres is mediated through protein kinase C. Neurosci Lett 91, 301–307.[CrossRef][Medline]
Ferreira J, Campos MM, Araujo R, Bader M, Pesquero JB & Calixto JB (2002). The use of kinin B1 and B2 receptor knockout mice and selective antagonists to characterize the nociceptive responses caused by kinins at the spinal level. Neuropharmacology 43, 1188–1197.[CrossRef][Medline]
Holzer P (2004). TRPV1 and the gut: from a tasty receptor for a painful vanilloid to a key player in hyperalgesia. Eur J Pharmacol 500, 231–241.[CrossRef][Medline]
Hori Y, Endo K & Takahashi T (1996). Long-lasting synaptic facilitation induced by serotonin in superficial dorsal horn neurones of the rat spinal cord. J Physiol 492, 867–876.[Medline]
Huang SM, Bisogno T, Trevisani M, Al-Hayani A, De Petrocellis L, Fezza F, Tognetto M, Petros TJ, Krey JF, Chu CJ, Miller JD, Davies SN, Geppetti P, Walker JM & Di Marzo V (2002). An endogenous capsaicin-like substance with high potency at recombinant and native vanilloid VR1 receptors. Proc Natl Acad Sci U S A 99, 8400–8405.
Huettner JE, Kerchner GA & Zhuo M (2002). Glutamate and the presynaptic control of spinal sensory transmission. Neuroscientist 8, 89–92.[Abstract]
Hwang SW, Cho H, Kwak J, Lee SY, Kang CJ, Jung J, Cho S, Min KH, Suh YG, Kim D & Oh U (2000). Direct activation of capsaicin receptors by products of lipoxygenases: endogenous capsaicin-like substances. Proc Natl Acad Sci U S A 97, 6155–6160.
Jung J, Shin JS, Lee SY, Hwang SW, Koo J, Cho H & Oh U (2004). Phosphorylation of vanilloid receptor 1 by Ca2+/calmodulin-dependent kinase II regulates its vanilloid binding. J Biol Chem 279, 7048–7054.
Karai L, Brown DC, Mannes AJ, Connelly ST, Brown J, Gandal M, Wellisch OM, Neubert JK, Olah Z & Iadarola MJ (2004). Deletion of vanilloid receptor 1-expressing primary afferent neurons for pain control. J Clin Invest 113, 1344–1352.[CrossRef][Medline]
Karlsson U, Sundgren-Andersson AK, Johansson S & Krupp JJ (2005). Capsaicin augments synaptic transmission in the rat medial preoptic nucleus. Brain Res 1043, 1–11.[CrossRef][Medline]
Kawasaki Y, Kohno T, Zhuang ZY, Brenner GJ, Wang H, Van Der Meer C, Befort K, Woolf CJ & Ji RR (2004). Ionotropic and metabotropic receptors, protein kinase A, protein kinase C, and Src contribute to C-fiber-induced ERK activation and cAMP response element-binding protein phosphorylation in dorsal horn neurons, leading to central sensitization. J Neurosci 24, 8310–8321.
Khasar SG, Lin YH, Martin A, Dadgar J, McMahon T, Wang D, Hundle B, Aley KO, Isenberg W, McCarter G, Green PG, Hodge CW, Levine JD & Messing RO (1999). A novel nociceptor signaling pathway revealed in protein kinase C epsilon mutant mice. Neuron 24, 253–260.[CrossRef][Medline]
Li P & Zhuo M (1998). Silent glutamatergic synapses and nociception in mammalian spinal cord. Nature 393, 695–698.[CrossRef][Medline]
Lou X, Scheuss V & Schneggenburger R (2005). Allosteric modulation of the presynaptic Ca2+ sensor for vesicle fusion. Nature 435, 497–501.[CrossRef][Medline]
Madrid R, Donovan-Rodriguez T, Meseguer V, Acosta MC, Belmonte C & Viana F (2006). Contribution of TRPM8 channels to cold transduction in primary sensory neurons and peripheral nerve terminals. J Neurosci 29, 12512–12525.
Malenka RC, Madison DV & Nicoll RA (1986). Potentiation of synaptic transmission in the hippocampus by phorbol esters. Nature 321, 175–177.[CrossRef][Medline]
Malmberg AB, Chen C, Tonegawa S & Basbaum AI (1997). Preserved acute pain and reduced neuropathic pain in mice lacking PKC. Science 278, 279–283.
Marinelli S, Di Marzo V, Berretta N, Matias I, Maccarrone M, Bernardi G & Mercuri NB (2003). Presynaptic facilitation of glutamatergic synapses to dopaminergic neurons of the rat substantia nigra by endogenous stimulation of vanilloid receptors. J Neurosci 23, 3136–3144.
Marinelli S, Vaughan CW, Christie MJ & Connor M (2002). Capsaicin activation of glutamatergic synaptic transmission in the rat locus coeruleus in vitro. J Physiol 543, 531–540.
Mohapatra DP & Nau C (2003). Desensitization of capsaicin-activated currents in the vanilloid receptor TRPV1 is decreased by the cyclic AMP-dependent protein kinase pathway. J Biol Chem 278, 50080–50090.
Muller D, Buchs PA, Stoppini L & Boddeke H (1991). Long-term potentiation, protein kinase C, and glutamate receptors. Mol Neurobiol 5, 277–288.[Medline]
Nakatsuka T, Furue H, Yoshimura M & Gu JG (2002). Activation of central terminal vanilloid receptor-1 receptors and 
-methylene-ATP-sensitive P2X receptors reveals a converged synaptic activity onto the deep dorsal horn neurons of the spinal cord. J Neurosci 22, 1228–1237.
Numazaki M, Tominaga T, Toyooka H & Tominaga M (2002). Direct phosphorylation of capsaicin receptor VR1 by protein kinase C and identification of two target serine residues. J Biol Chem 277, 13375–13378.
Premkumar LS & Ahern GP (2000). Induction of vanilloid receptor channel activity by protein kinase C. Nature 408, 985–990.[CrossRef][Medline]
Premkumar LS, Qi ZH, Van Buren J & Raisinghani M (2004). Enhancement of potency and efficacy of NADA by PKC-mediated phosphorylation of vanilloid receptor. J Neurophysiol 91, 1442–1449.
Raisinghani M, Pabbidi RM & Premkumar LS (2005). Activation of transient receptor potential vanilloid 1 (TRPV1) by resiniferatoxin. J Physiol 567, 771–786.
Rhee JS, Betz A, Pyott S, Reim K, Varoqueaux F, Augustin I, Hesse D, Sudhof TC, Takahashi M, Rosenmund C & Brose N (2002). phorbol ester- and diacylglycerol-induced augmentation of transmitter release is mediated by Muncl13s and not by PKCs. Ce
