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Erratum for Taylor et al., J Physiol 576 (3) 769-785.
J Physiol Volume 579, Number 1, 285-, February 15, 2007 DOI: 10.1113/jphysiol.2006.578101
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Errata

The paper by Taylor et al. (2006) contains errors to Table 5 on page 781

The table should have appeared as follows:


Table 5.  Comparison of transport properties for vascular endothelia
Preparation Umbilical vein endothelial cells Aortic endothelial cells Pulmonary artery endothelial cells Brain microvascular endothelial cells
Other studies This study

Basal pH with HCO3 7.26 ~7.2 6.9, 7.18** 7.15
Basal pH without HCO3 7.21 ~7.2, 7.08* 7.02 6.83 7.1
Intrinsic buffer capacity near 7.1 15 mM 69 mM 25 mM
Near pH 7.1
 Na+/H+ exchange ~17 µM s–1 Yes Yes*, yes** ~4 µM s–1
 Na+-dependent HCO3 influx yes Yes ~25 µM s–1
  Cl dependent ~17 µM s–1 suggestion
  Cl independent ~17 µM s–1
 Na+-independent Cl/HCO3 Yes See note* {dagger} ~28 µM s–1 when
 exchange reversed by Cl removal
After NH4Cl prepulse#. Yes*
 Na+/H+ exchange > 50 µM s–1 at pH 6.9 Yes* Yes Yes, yes** ~270 µM s–1 at pH 6.36
 Na+-dependent HCO3 influx Yes Yes* Yes
  Cl dependent > 34 µM s–1 at pH 6.9 Yes* Yes but data not shown
Net transport None None None secretion: apical (blood) to basolateral (brain)
References Sun et al. (1999) Ziegelstein et al. (1992) Cutaia & Parks (1996) Sipos et al. (2005) This study
Escobales et al. (1990)* Ziegelstein et al. (1998) Cutaia et al. (1998) Vigne et al. (1991)*
Wittstein et al. (2000) Nozik-Grayck et al. (2003)* Hsu et al. (1996)**
Faber (1998)*

All HCO3 transport indicated has been shown to be either DIDS or H2DIDS sensitive, all Na+/H+ exchange has been shown to be sensitive to either EIPA or DMA, (#) or other form of imposed acidification. {dagger}Nozik-Grayck et al. (2003) demonstrated ·O2/HCO3 exchange which was presumed to be mediated by the Na+-independent Cl/HCO3 exchanger, AE2.

Also on page 776, right column, the increase in pH cited on the 5th and 4th line from the bottom should read: 0.63 ± 0.06 to 0.17 ± 0.04 by 2 µM ethoxzolamide and to 0.18 ± 0.02


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Taylor CJ, Nicola PA, Wang S, Barrand MA & Hladky SB (2006). Transporters involved in regulation of intracellular pH in primary cultured rat brain endothelial cells. J Physiol 576, 769–785.[Abstract/Free Full Text]





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