| Preparation |
Umbilical vein endothelial cells |
Aortic endothelial cells |
Pulmonary artery endothelial cells |
Brain microvascular endothelial cells
|
| Other studies |
This study |
|
| Basal pH with HCO3 |
7.26 |
7.2 |
|
6.9, 7.18** |
7.15 |
| Basal pH without HCO3 |
7.21 |
7.2, 7.08* |
7.02 |
6.83 |
7.1 |
| Intrinsic buffer capacity near 7.1 |
15 mM |
|
69 mM |
|
25 mM |
| Near pH 7.1 |
| Na+/H+ exchange |
17 µM s1 |
Yes |
|
Yes*, yes** |
4 µM s1 |
| Na+-dependent HCO3 influx |
yes |
Yes |
|
25 µM s1 |
| Cl dependent |
17 µM s1 |
|
suggestion |
| Cl independent |
|
17 µM s1 |
| Na+-independent Cl/HCO3 |
|
Yes |
See note*
 |
|
28 µM s1 when |
| exchange |
|
reversed by Cl removal |
| After NH4Cl prepulse#. |
Yes* |
|
| Na+/H+ exchange |
> 50 µM s1 at pH 6.9 |
Yes* |
Yes |
Yes, yes** |
270 µM s1 at pH 6.36 |
| Na+-dependent HCO3 influx |
Yes |
Yes* |
|
Yes |
|
| Cl dependent |
> 34 µM s1 at pH 6.9 |
Yes* |
|
Yes but data not shown |
|
| Net transport |
None |
None |
None |
secretion: apical (blood) to basolateral (brain) |
| References |
Sun et al. (1999) |
Ziegelstein et al. (1992) |
Cutaia & Parks (1996) |
Sipos et al. (2005) |
This study |
| Escobales et al. (1990)* |
Ziegelstein et al. (1998) |
Cutaia et al. (1998) |
Vigne et al. (1991)* |
|
| Wittstein et al. (2000) |
Nozik-Grayck et al. (2003)* |
Hsu et al. (1996)** |
|
| Faber (1998)* |
|
|
All HCO3 transport indicated has been shown to be either DIDS or H2DIDS sensitive, all Na+/H+ exchange has been shown to be sensitive to either EIPA or DMA, (#) or other form of imposed acidification. Nozik-Grayck et al. (2003) demonstrated ·O2/HCO3 exchange which was presumed to be mediated by the Na+-independent Cl/HCO3 exchanger, AE2. |