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PERSPECTIVES |
1 Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada N1G 2W1
Email: mlinding{at}uoguelph.ca
During strenuous exercise, contracting skeletal muscle fatigues at a high rate in part due to ionic imbalances occurring across the sarcolemma that contribute to decreased sarcolemmal excitatibility (Sjogaard, 1990). It is suspected that the reduced excitability contributes to a reduction in calcium release by the sarcoplasmic reticulum and a consequent decrease in the force of muscle contraction (Lindinger, 2006). The contribution of this membrane mechanism to the fatigue of high-intensity exercise has been proposed as a way of protecting contracting muscle cells against the damage that may occur if contraction were to continue (Sjogaard, 1990). The accumulation of lactate and especially associated protons, both within the cell and in the extracellular fluids surrounding cells (Lindinger et al. 2005), have also long been thought to contribute to the fatigue of high-intensity exercise. However, Nielsen et al. (2001) previously raised serious questions about the roles of lactate and acidosis in the fatigue process when they showed, in rat muscle in vitro, that acidosis counteracted the effects of increased extracellular [K+] ([K+]o) on sarcolemmal Vm. So, while the effects of increased [K+]o as a key contributor to the fatigue of high-intensity exercise remain unrefuted, the elevated extracellular [H+] of exercise appears to allow muscle to continue to contract despite a higher [K+]o. Further, the authors have gone on to state: ‘In many kinds of exercise, reduced excitability may never contribute to fatigue because endogenous protection via activation of the Na+–K+ pumps and/or development of acidosis is sufficient to maintain excitability’ (Nielsen & Overgaard, 2006). It is noteworthy that other ‘protective’ effects of lactate on sustaining muscle contraction are also recognized (Brooks, 2001; Cairns, 2006).
In this issue of The Journal of Physiology, Nielsen and coworkers (de Paoli et al. 2007) provide support that extracellular lactate accumulation has a protective effect on muscle excitability. They demonstrated that an increased extracellular acidity (20 mmol l–1 lactic acid), similar to that seen during high-intensity exercise, is additive to the effects of elevated adrenaline on stabilizing membrane excitability. Most importantly, the combination of these two effects was able to fully restore muscle force production. In contrast, adrenaline without concurrent increase in extracellular acidity, through its effects of increasing sarcolemmal Na+–K+-ATPase activity and hence membrane potential, was not able to fully restore sarcolemmal excitability or muscle force production.
Limitations of the studies to date is that they have been performed on isolated fibre bundles or fibres, typically at room temperature in vitro, and the manipulations performed only mimic a few of the key changes that occur during high-intensity muscle contractions. Another caveat is that the influence of elevated [H+]o on fatigue of muscle in vitro is pronounced at low temperature but not at physiological temperatures; however, this temperature effect is not pronounced at elevated intracellular [H+] (Knuth et al. 2006). It remains unknown if the mechanisms that operate in such controlled conditions in vitro occur to a similar degree within complex in vivo systems. In the exercising human, there occur simultaneous changes in both the intra- and extracellular ionic and metabolic environments. So while the in vitro studies provide insight as to what may occur, we should still ask the question: Why should we expect the results of the present study to be similar to what occurs in contracting muscle in vivo? I am sure that we all look forward to finding out the answers.
References
Brooks GA (2001). Lactate doesn't necessarily cause fatigue: why are we surprised? J Physiol 536, 1.
Cairns SP (2006). Lactic acid and exercise performance – culprit or friend? Sports Med 36, 279–291.[CrossRef][Medline]
de Paoli FV, Overgaard K, Pedersen TH & Nielsen OB (2007). Additive protective effects of the addition of lactic acid and adrenaline on excitability and force in isolated rat skeletal muscle depressed by elevated extracellular K+. J Physiol 581, 829–839.
Knuth ST, Dave H, Peters JR & Fitts RH (2006). Low cell pH depresses peak power in rat skeletal muscle fibres at both 30°C and 15°C: implications for muscle fatigue. J Physiol 575, 887–899.
Lindinger MI (2006). Determinants of sarcolemmal and transverse tubular excitability in skeletal muscle: implications for high intensity exercise. Equine Comp Exerc Physiol 2, 209–217.
Lindinger MI, Kowalchuk JM & Heigenhauser GJ (2005). Applying physicochemical principles to skeletal muscle acid-base status. Am J Physiol Regul Integr Comp Physiol 289, R891–R894.
Nielsen OB, de Paoli F, Overgaard K (2001). Protective effects of lactic acid on force production in rat skeletal muscle. J Physiol 536, 161–166.
Nielsen OB & Overgaard K (2006). Point:Counterpoint authors respond to commentaries on ‘Lactic acid accumulation is an advantage/disadvantage during muscle activity’. J Appl Physiol 101, 367.
Sjogaard G (1990). Exercise-induced muscle fatigue: the significance of potassium. Acta Physiol Scand Suppl 593, 1–63.[Medline]
Related Article
J. Physiol. 2007 581: 829-839.
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