| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Received June 21, 2001
Accepted after revision December 17, 2001
1 Department of Cell and Molecular Physiology, University of North Carolina-CH, Chapel Hill, NC 27599-7545, USA
* To whom correspondence should be addressed. E-mail: erp{at}med.unc.edu.
Relationships between the morphology of individual neurones of the spinal superficial dorsal horn (SDH), laminae I and II, and their electrophysiological properties were studied in spinal cord slices prepared from anaesthetized, free-ranging hamsters. Tight-seal, whole-cell recordings were made with pipette microelectrodes filled with biocytin to establish electrophysiological characteristics and to label the studied neurones. Neurones were categorized according to location and size of the somata, the dendritic and axonal pattern of arborization, spontaneous synaptic potentials, evoked postsynaptic currents, pattern of discharge to depolarizing pulses and current-voltage relationships. Data were obtained for 170 neurones; 13 of these had somata in lamina I and 157 in lamina II. Stimulation of the segmental dorsal root evoked a prompt excitatory response in almost every neurone sampled (161/166) with nearly 3/4 displaying putative monosynaptic EPSCs. The majority of neurones (133/170) fitted one of several distinctive morphological categories. To a considerable extent, neurones with a common morphological configuration and neurite disposition shared electrophysiological characteristics. Five of the 13 lamina I neurones were relatively large with extensive dendritic arborization in the horizontal dimension and a prominent axon directed ventrally and contralaterally. These presumptive ventrolateral projection neurones differed structurally and electrophysiologically from the other lamina I neurones, which had ipsilateral, locally arborizing axons and/or branches entering the dorsal lateral funiculus. One hundred and twenty lamina II neurones fitted one of five morphological categories: islet, central, medial-lateral, radial or vertical. Central cells were further divided into three groups on functional features. We conclude that the spinal SDH comprises many types of neurones whose morphological characteristics are associated with specific functional features implying diversity in functional organization of the SDH and in its role as a major synaptic termination for thin primary afferent fibres.
This article has been cited by other articles:
|
|
 |
|
  Y. Lu, J. Zheng, L. Xiong, M. Zimmermann, and J. Yang Spinal cord injury-induced attenuation of GABAergic inhibition in spinal dorsal horn circuits is associated with down-regulation of the chloride transporter KCC2 in rat J. Physiol., December 1, 2008; 586(23): 5701 - 5715. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-Y. Peng, H.-M. Chang, S. Y. Chang, K.-C. Tung, S.-D. Lee, D. Chou, C.-Y. Lai, C.-H. Chiu, G.-D. Chen, and T.-B. Lin Orexin-A modulates glutamatergic NMDA-dependent spinal reflex potentiation via inhibition of NR2B subunit Am J Physiol Endocrinol Metab, July 1, 2008; 295(1): E117 - E129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Graham, A. M. Brichta, and R. J. Callister Recording Temperature Affects the Excitability of Mouse Superficial Dorsal Horn Neurons, In Vitro J Neurophysiol, May 1, 2008; 99(5): 2048 - 2059. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Xu, C. Lopes, Y. Qian, Y. Liu, L. Cheng, M. Goulding, E. E. Turner, D. Lima, and Q. Ma Tlx1 and Tlx3 Coordinate Specification of Dorsal Horn Pain-Modulatory Peptidergic Neurons J. Neurosci., April 9, 2008; 28(15): 4037 - 4046. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. W. Bailey, S. M. Appleyard, Y.-H. Jin, and M. C. Andresen Organization and Properties of GABAergic Neurons in Solitary Tract Nucleus (NTS) J Neurophysiol, April 1, 2008; 99(4): 1712 - 1722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Liu, T. Fujita, T. Nakatsuka, and E. Kumamoto Phospholipase A2 Activation Enhances Inhibitory Synaptic Transmission in Rat Substantia Gelatinosa Neurons J Neurophysiol, March 1, 2008; 99(3): 1274 - 1284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Pinto, V. A. Derkach, and B. V. Safronov Role of TTX-Sensitive and TTX-Resistant Sodium Channels in A{delta}- and C-Fiber Conduction and Synaptic Transmission J Neurophysiol, February 1, 2008; 99(2): 617 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Maxwell, M. D. Belle, O. Cheunsuang, A. Stewart, and R. Morris Morphology of inhibitory and excitatory interneurons in superficial laminae of the rat dorsal horn J. Physiol., October 15, 2007; 584(2): 521 - 533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. B. Lu, K. Ballanyi, W. F. Colmers, and P. A. Smith Neuron type-specific effects of brain-derived neurotrophic factor in rat superficial dorsal horn and their relevance to 'central sensitization' J. Physiol., October 15, 2007; 584(2): 543 - 563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Graham, A. M. Brichta, P. R. Schofield, and R. J. Callister Altered potassium channel function in the superficial dorsal horn of the spastic mouse J. Physiol., October 1, 2007; 584(1): 121 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Graham, A. M. Brichta, and R. J. Callister Moving From an Averaged to Specific View of Spinal Cord Pain Processing Circuits J Neurophysiol, September 1, 2007; 98(3): 1057 - 1063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Lu and E. R. Perl Selective action of noradrenaline and serotonin on neurones of the spinal superficial dorsal horn in the rat J. Physiol., July 1, 2007; 582(1): 127 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yasaka, G. Kato, H. Furue, M. H. Rashid, M. Sonohata, A. Tamae, Y. Murata, S. Masuko, and M. Yoshimura Cell-type-specific excitatory and inhibitory circuits involving primary afferents in the substantia gelatinosa of the rat spinal dorsal horn in vitro J. Physiol., June 1, 2007; 581(2): 603 - 618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. A. Santos, S. Rebelo, V. A. Derkach, and B. V. Safronov Excitatory interneurons dominate sensory processing in the spinal substantia gelatinosa of rat J. Physiol., May 15, 2007; 581(1): 241 - 254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Kato, Y. Kawasaki, R.-R. Ji, and A. M. Strassman Differential wiring of local excitatory and inhibitory synaptic inputs to islet cells in rat spinal lamina II demonstrated by laser scanning photostimulation J. Physiol., May 1, 2007; 580(3): 815 - 833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Berkowitz Spinal Interneurons That Are Selectively Activated during Fictive Flexion Reflex J. Neurosci., April 25, 2007; 27(17): 4634 - 4641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Graham, A. M. Brichta, and R. J. Callister Pinch-current injection defines two discharge profiles in mouse superficial dorsal horn neurones, in vitro J. Physiol., February 1, 2007; 578(3): 787 - 798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Schoffnegger, B. Heinke, C. Sommer, and J. Sandkuhler Physiological properties of spinal lamina II GABAergic neurons in mice following peripheral nerve injury J. Physiol., December 15, 2006; 577(3): 869 - 878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Balasubramanyan, P. L. Stemkowski, M. J. Stebbing, and P. A. Smith Sciatic Chronic Constriction Injury Produces Cell-Type-Specific Changes in the Electrophysiological Properties of Rat Substantia Gelatinosa Neurons J Neurophysiol, August 1, 2006; 96(2): 579 - 590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Kuo, R. H. Lee, L. Zhang, and C. J. Heckman Essential role of the persistent sodium current in spike initiation during slowly rising inputs in mouse spinal neurones J. Physiol., August 1, 2006; 574(3): 819 - 834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Brown, S. Henning, and C. L. Wellman Mild, Short-term Stress Alters Dendritic Morphology in Rat Medial Prefrontal Cortex Cereb Cortex, November 1, 2005; 15(11): 1714 - 1722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Prescott and Y. De Koninck Integration Time in a Subset of Spinal Lamina I Neurons Is Lengthened by Sodium and Calcium Currents Acting Synergistically to Prolong Subthreshold Depolarization J. Neurosci., May 11, 2005; 25(19): 4743 - 4754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Lu and E. R. Perl Modular Organization of Excitatory Circuits between Neurons of the Spinal Superficial Dorsal Horn (Laminae I and II) J. Neurosci., April 13, 2005; 25(15): 3900 - 3907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A Graham, A. M Brichta, and R. J Callister In vivo responses of mouse superficial dorsal horn neurones to both current injection and peripheral cutaneous stimulation J. Physiol., December 15, 2004; 561(3): 749 - 763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yang, T. Fujita, and E. Kumamoto Adenosine Inhibits GABAergic and Glycinergic Transmission in Adult Rat Substantia Gelatinosa Neurons J Neurophysiol, November 1, 2004; 92(5): 2867 - 2877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Heinke, R. Ruscheweyh, L. Forsthuber, G. Wunderbaldinger, and J. Sandkuhler Physiological, neurochemical and morphological properties of a subgroup of GABAergic spinal lamina II neurones identified by expression of green fluorescent protein in mice J. Physiol., October 1, 2004; 560(1): 249 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Kato, H. Furue, T. Katafuchi, T. Yasaka, Y. Iwamoto, and M. Yoshimura Electrophysiological mapping of the nociceptive inputs to the substantia gelatinosa in rat horizontal spinal cord slices J. Physiol., October 1, 2004; 560(1): 303 - 315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. V. Melnick, S. F. A. Santos, and B. V. Safronov Mechanism of spike frequency adaptation in substantia gelatinosa neurones of rat J. Physiol., September 1, 2004; 559(2): 383 - 395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-Z. Pan and H.-L. Pan Primary Afferent Stimulation Differentially Potentiates Excitatory and Inhibitory Inputs to Spinal Lamina II Outer and Inner Neurons J Neurophysiol, June 1, 2004; 91(6): 2413 - 2421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. V. Melnick, S. F. A. Santos, K. Szokol, P. Szucs, and B. V. Safronov Ionic Basis of Tonic Firing in Spinal Substantia Gelatinosa Neurons of Rat J Neurophysiol, February 1, 2004; 91(2): 646 - 655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Hantman, A. N. van den Pol, and E. R. Perl Morphological and Physiological Features of a Set of Spinal Substantia Gelatinosa Neurons Defined by Green Fluorescent Protein Expression J. Neurosci., January 28, 2004; 24(4): 836 - 842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Y. Yang, T. Verhovshek, and D. R. Sengelaub Brain-Derived Neurotrophic Factor and Androgen Interact in the Maintenance of Dendritic Morphology in a Sexually Dimorphic Rat Spinal Nucleus Endocrinology, January 1, 2004; 145(1): 161 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Lu and E. R. Perl A Specific Inhibitory Pathway between Substantia Gelatinosa Neurons Receiving Direct C-Fiber Input J. Neurosci., September 24, 2003; 23(25): 8752 - 8758. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-J. Hu, K. S. Glauner, and R. W. Gereau IV ERK Integrates PKA and PKC Signaling in Superficial Dorsal Horn Neurons. I. Modulation of A-Type K+ Currents J Neurophysiol, September 1, 2003; 90(3): 1671 - 1679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-J. Hu and R. W. Gereau IV ERK Integrates PKA and PKC Signaling in Superficial Dorsal Horn Neurons. II. Modulation of Neuronal Excitability J Neurophysiol, September 1, 2003; 90(3): 1680 - 1688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Schneider Spike Frequency Adaptation and Signaling Properties of Identified Neurons in Rodent Deep Spinal Dorsal Horn J Neurophysiol, July 1, 2003; 90(1): 245 - 258. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
