| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Received July 25, 2001
Accepted after revision November 2, 2001
1 Laboratoire de Signalisation et Interactions Cellulaires, CNRS UMR 5017, Université Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux, France
2 Molecular Medicine Section, Department of Neuroscience, University of Sienna and DIBIT, Istituto Scientifico San Raffaele, Via Olgettina 58, 20132 Milano, Italy
* To whom correspondence should be addressed. E-mail: jean.mironneau{at}umr5017.u-bordeaux2.fr.
Subtype 3 of the ryanodine receptor (RYR3) is a ubiquitous Ca2+ release channel which is predominantly expressed in smooth muscle tissues and certain regions of the brain. We show by reverse transcription-polymerase chain reaction (RT-PCR) that non-pregnant mouse myometrial cells expressed only RYR3 and therefore could be a good model for studying the role of endogenous RYR3. Expression of RYR3 was confirmed by Western blotting and immunostaining. Confocal Ca2+ measurements revealed that in 1.7 mm extracellular Ca2+, neither caffeine nor photolysis of caged Ca2+ were able to trigger any Ca2+ responses, whereas in the same cells oxytocin activated propagated Ca2+ waves. However, under conditions of increased sarcoplasmic reticulum (SR) Ca2+ loading, brought about by superfusing myometrial cells in 10 mm extracellular Ca2+, all the myometrial cells responded to caffeine and photolysis of caged Ca2+, indicating that it was possible to activate RYR3. The caffeine-induced Ca2+ responses were inhibited by intracellular application of an anti-RYR3-specific antibody. Immunodetection of RYR3 with the same antibody revealed a rather homogeneous distribution of fluorescence in confocal cell sections. In agreement with these observations, spontaneous or triggered Ca2+ sparks were not detected. In conclusion, our results suggest that under conditions of increased SR Ca2+ loading, endogenous RYR3 may contribute to the Ca2+ responses of myometrial cells.
This article has been cited by other articles:
|
|
 |
|
  F. Dabertrand, N. Fritz, J. Mironneau, N. Macrez, and J.-L. Morel Role of RYR3 splice variants in calcium signaling in mouse nonpregnant and pregnant myometrium Am J Physiol Cell Physiol, September 1, 2007; 293(3): C848 - C854. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wray Insights into the uterus Exp Physiol, July 1, 2007; 92(4): 621 - 631. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Laporte, A. Hui, and I. Laher Pharmacological Modulation of Sarcoplasmic Reticulum Function in Smooth Muscle Pharmacol. Rev., December 1, 2004; 56(4): 439 - 513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Chiarella, R. Puglisi, V. Sorrentino, C. Boitani, and M. Stefanini Ryanodine receptors are expressed and functionally active in mouse spermatogenic cells and their inhibition interferes with spermatogonial differentiation J. Cell Sci., August 15, 2004; 117(18): 4127 - 4134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Aoyama, A. Yamada, J. Wang, S. Ohya, S. Furuzono, T. Goto, S. Hotta, Y. Ito, T. Matsubara, K. Shimokata, et al. Requirement of ryanodine receptors for pacemaker Ca2+ activity in ICC and HEK293 cells J. Cell Sci., June 1, 2004; 117(13): 2813 - 2825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Morel, L. Rakotoarisoa, L. H. Jeyakumar, S. Fleischer, C. Mironneau, and J. Mironneau Decreased Expression of Ryanodine Receptors Alters Calcium-induced Calcium Release Mechanism in mdx Duodenal Myocytes J. Biol. Chem., May 14, 2004; 279(20): 21287 - 21293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Jiang, B. Xiao, X. Li, and S. R. W. Chen Smooth Muscle Tissues Express a Major Dominant Negative Splice Variant of the Type 3 Ca2+ Release Channel (Ryanodine Receptor) J. Biol. Chem., February 7, 2003; 278(7): 4763 - 4769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Xiao, H. Masumiya, D. Jiang, R. Wang, Y. Sei, L. Zhang, T. Murayama, Y. Ogawa, F. A. Lai, T. Wagenknecht, et al. Isoform-dependent Formation of Heteromeric Ca2+ Release Channels (Ryanodine Receptors) J. Biol. Chem., October 25, 2002; 277(44): 41778 - 41785. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
