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Received September 21, 2001
Accepted after revision October 30, 2001
1 Department of Otolaryngology, Center for Neuroscience, University of California, Davis, 1544 Newton Court, Davis, CA 95616, USA
2 Department of Physiology and Biophysics, University of Miami School of Medicine, Miami, FL 60612, USA
3 University of California, Davis, Center for Neuroscience, Department of Otolaryngology, 1544 Newton Court, Davis, CA 95616, USA
* To whom correspondence should be addressed. E-mail: enyamoah{at}ucdavis.edu.
We studied the permeation properties of two distinct single voltage-gated Ca2+ channels in bullfrog saccular hair cells to assess the roles of the channels as physiological Ca2+ transporters and multi-ion pores. By varying the permeant ions (Ba2+, Ca2+) and concentrations (2-70 mm), we estimated the affinity constant (KD) of the two channels as follows (mm): L-type channel, KD,Ba = 7.4 ± 1.0, KD,Ca = 7.1 ± 2.2 (n = 7); non-L-type channel, KD,Ba = 5.3 ± 3.2, KD,Ca = 2.0 ± 1.0 (n = 8). Using ionic concentrations close to physiological conditions (2 mm Ca2+ and 0.5 mm Mg2+), the conductance of the L-type channel was ~2 pS. We determined the mechanisms by which ions traverse the pore of these single Ca2+ channels, using mixtures of Ba2+ and Ca2+ at total concentrations above (70 mm) or close to (5 mm) the KD of the channels. We found evidence for an anomalous mole fraction effect (AMFE) only when the total divalent ion concentration was 5 mm, consistent with a multi-ion pore. We show that AMFE arises from the boundaries between the pore and bulk solution in the atria of the channel, which is derived from the presence of depletion zones that become apparent at low divalent cation concentrations. The present findings provide an explanation as to why previous whole-cell Ca2+ currents that were recorded in quasi-physiological Ca2+ concentrations (~2-5 mm) showed clear AMFE, whereas single Ca2+ channel currents that were recorded routinely at high Ca2+ concentrations (20-110 mm) did not.
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