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Received October 11, 2001
Accepted after revision December 5, 2001
1 Synaptic Mechanisms Section, NINDS, NIH, Bethesda MD 20892-4156, USA
2 Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston TX 77030, USA
3 Toronto Western Research Institute, Toronto, Ontario, Canada M5T 2S8
4 MP14-320, Toronto Western Research Institute, 399 Bathurst Street, Toronto ON M5T 2S8, Canada
* To whom correspondence should be addressed. E-mail: estanley{at}uhnres.utoronto.ca.
The isolated chick ciliary neuron calyx synapse preparation was used to test cysteine string protein (CSP) action on presynaptic Ca2+ channels. Endogenous CSP was localized primarily to secretory vesicle clusters in the presynaptic nerve terminal. Introduction of recombinant CSP into the voltage clamped terminal resulted in a prominent increase in Ca2+ current amplitude. However, this increase could not be attributed to a change in Ca2+ channel kinetics, voltage dependence, prepulse inactivation, or G protein inhibition but was attributed to the recruitment of dormant channels. Endogenous CSP may play an important role in enhancing Ca2+ channel activity at the transmitter release site.
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