| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Received February 27, 2002
Accepted after revision May 24, 2002
1 Département de Physiologie, Faculté de Médecine, 11, rue Humann, 67000 Strasbourg, France
2 Unité de Bioénergétique, CRSSA, BP 87, 38702 La-Tronche Cedex, Franc
3 Département de Physiologie, Equipe d'Accueil 3072, Faculté de Médecine, Université Louis Pasteur, Strasbourg, France
4 Département de Physiologie, Equipe d'Accueil 3072, Faculté de Médecine, Université Louis Pasteur, Strasbourge, France
5 Unité de Bioénergétique, CRSSA, BP 87, 38702 La-Tronche Cedex, France
6 Cardiologie Cellulaire et Moléculaire U-446 INSERM, Faculté de Pharmacie, Université Paris-Sud, 92296 Chatenay-Malabry, France
7 Département de Physiologie, Equipe d'Accueil 3072, Faculté de Médecine, Université Louis Pasteur, Strasbourg,, France
* To whom correspondence should be addressed. E-mail: Joffrey.Zoll{at}chru-strasbourg.fr.
This study explores the importance of creatine kinase (CK) in the regulation of muscle mitochondrial respiration in human subjects depending on their level of physical activity. Volunteers were classified as sedentary, active or athletic according to the total activity index as determined by the Baecke questionnaire in combination with maximal oxygen uptake values (peak VO2 , expressed in ml min-1 kg-1). All volunteers underwent a cyclo-ergometric incremental exercise test to estimate their peak VO2 and VO2 at the ventilatory threshold (VT). Muscle biopsy samples were taken from the vastus lateralis and mitochondrial respiration was evaluated in an oxygraph cell on saponin permeabilised muscle fibres in the absence (V) or in the presence (Vmax) of saturating [ADP]. While V was similar, Vmax differed among groups (sedentary, 3.7 ± 0.3, active, 5.9 ± 0.9 and athletic, 7.9 ± 0.5 µmol O2 min-1 (g dry weight)-1). Vmax was correlated with peak VO2 (P < 0.01, r = 0.63) and with VT (P < 0.01, r = 0.57). There was a significantly greater degree of coupling between oxidation and phosphorylation (Vmax/V) in the athletic individuals. The mitochondrial Km for ADP was significantly higher in athletic subjects (P < 0.01). Mitochondrial CK (mi-CK) activation by addition of creatine induced a marked decrease in Km in Ath individuals only, indicative of an efficient coupling of mi-CK to ADP rephosphorylation in the athletic subjects only. It is suggested that increasing aerobic performance requires an enhancement of both muscle oxidative capacity and mechanisms of respiratory control, attesting to the importance of temporal co-ordination of energy fluxes by CK for higher efficacy.
This article has been cited by other articles:
|
|
 |
|
  F. N. Daussin, J. Zoll, S. P. Dufour, E. Ponsot, E. Lonsdorfer-Wolf, S. Doutreleau, B. Mettauer, F. Piquard, B. Geny, and R. Richard Effect of interval versus continuous training on cardiorespiratory and mitochondrial functions: relationship to aerobic performance improvements in sedentary subjects Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2008; 295(1): R264 - R272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. N. Daussin, J. Zoll, E. Ponsot, S. P. Dufour, S. Doutreleau, E. Lonsdorfer, R. Ventura-Clapier, B. Mettauer, F. Piquard, B. Geny, et al. Training at high exercise intensity promotes qualitative adaptations of mitochondrial function in human skeletal muscle J Appl Physiol, May 1, 2008; 104(5): 1436 - 1441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Richard, J. Zoll, B. Mettauer, F. Piquard, and B. Geny Counterpoint: Cardiac denervation does not play a major role in exercise limitation after heart transplantation J Appl Physiol, February 1, 2008; 104(2): 560 - 562. [Full Text] [PDF]
|
|
|
|
 |
|
 O. Rouyer, J. Zoll, F. Daussin, C. Damge, P. Helms, S. Talha, L. Rasseneur, F. Piquard, and B. Geny Muscle: Effect of angiotensin-converting enzyme inhibition on skeletal muscle oxidative function and exercise capacity in streptozotocin-induced diabetic rats Exp Physiol, November 1, 2007; 92(6): 1047 - 1056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Roels, C. Thomas, D. J. Bentley, J. Mercier, M. Hayot, and G. Millet Effects of intermittent hypoxic training on amino and fatty acid oxidative combustion in human permeabilized muscle fibers J Appl Physiol, January 1, 2007; 102(1): 79 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sahlin, M. Mogensen, M. Bagger, M. Fernstrom, and P. K. Pedersen The potential for mitochondrial fat oxidation in human skeletal muscle influences whole body fat oxidation during low-intensity exercise Am J Physiol Endocrinol Metab, January 1, 2007; 292(1): E223 - E230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ventura-Clapier, B. Mettauer, and X. Bigard Beneficial effects of endurance training on cardiac and skeletal muscle energy metabolism in heart failure Cardiovasc Res, January 1, 2007; 73(1): 10 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Hannukainen, P. Nuutila, J. Kaprio, O. J Heinonen, U. M. Kujala, T. Janatuinen, T. Ronnemaa, J. Kapanen, M. Haaparanta-Solin, T. Viljanen, et al. Relationship between local perfusion and FFA uptake in human skeletal muscle--no effect of increased physical activity and aerobic fitness J Appl Physiol, November 1, 2006; 101(5): 1303 - 1311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zoll, L. Monassier, A. Garnier, B. N'Guessan, B. Mettauer, V. Veksler, F. Piquard, R. Ventura-Clapier, and B. Geny ACE inhibition prevents myocardial infarction-induced skeletal muscle mitochondrial dysfunction J Appl Physiol, August 1, 2006; 101(2): 385 - 391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ostergard, J. L. Andersen, B. Nyholm, S. Lund, K.S. Nair, B. Saltin, and O. Schmitz Impact of exercise training on insulin sensitivity, physical fitness, and muscle oxidative capacity in first-degree relatives of type 2 diabetic patients Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E998 - E1005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Eimre, R. Puhke, K. Alev, E. Seppet, A. Sikkut, N. Peet, L. Kadaja, A. Lenzner, T. Haviko, T. Seene, et al. Altered mitochondrial apparent affinity for ADP and impaired function of mitochondrial creatine kinase in gluteus medius of patients with hip osteoarthritis Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1271 - R1275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Ponsot, S. P. Dufour, J. Zoll, S. Doutrelau, B. N'Guessan, B. Geny, H. Hoppeler, E. Lampert, B. Mettauer, R. Ventura-Clapier, et al. Exercise training in normobaric hypoxia in endurance runners. II. Improvement of mitochondrial properties in skeletal muscle J Appl Physiol, April 1, 2006; 100(4): 1249 - 1257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Guerrero, B. Wuyam, P. Mezin, I. Vivodtzev, M. Vendelin, J.-C. Borel, R. Hacini, O. Chavanon, S. Imbeaud, V. Saks, et al. Functional coupling of adenine nucleotide translocase and mitochondrial creatine kinase is enhanced after exercise training in lung transplant skeletal muscle Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2005; 289(4): R1144 - R1154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Garnier, D. Fortin, J. Zoll, B. N'Guessan, B. Mettauer, E. Lampert, V. Veksler, and R. Ventura-Clapier Coordinated changes in mitochondrial function and biogenesis in healthy and diseased human skeletal muscle FASEB J, January 1, 2005; 19(1): 43 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Thomas, P. Sirvent, S. Perrey, E. Raynaud, and J. Mercier Relationships between maximal muscle oxidative capacity and blood lactate removal after supramaximal exercise and fatigue indexes in humans J Appl Physiol, December 1, 2004; 97(6): 2132 - 2138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ventura-Clapier, A. Garnier, and V. Veksler Energy metabolism in heart failure J. Physiol., February 15, 2004; 555(1): 1 - 13. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Nuhr, D. Pette, R. Berger, M. Quittan, R. Crevenna, M. Huelsman, G. F. Wiesinger, P. Moser, V. Fialka-Moser, and R. Pacher Beneficial effects of chronic low-frequency stimulation of thigh muscles in patients with advanced chronic heart failure Eur. Heart J., January 2, 2004; 25(2): 136 - 143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Bahi, N. Koulmann, H. Sanchez, I. Momken, V. Veksler, A. X. Bigard, and R. Ventura-Clapier Does ACE inhibition enhance endurance performance and muscle energy metabolism in rats? J Appl Physiol, January 1, 2004; 96(1): 59 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zoll, B. N'Guessan, F. Ribera, E. Lampert, D. Fortin, V. Veksler, X. Bigard, B. Geny, J. Lonsdorfer, R. Ventura-Clapier, et al. Preserved response of mitochondrial function to short-term endurance training in skeletal muscle of heart transplant recipients J. Am. Coll. Cardiol., July 2, 2003; 42(1): 126 - 132. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Ribera, B. N'Guessan, J. Zoll, D. Fortin, B. Serrurier, B. Mettauer, X. Bigard, R. Ventura-Clapier, and E. Lampert Mitochondrial Electron Transport Chain Function Is Enhanced in Inspiratory Muscles of Patients with Chronic Obstructive Pulmonary Disease Am. J. Respir. Crit. Care Med., March 15, 2003; 167(6): 873 - 879. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
