J Physiol Boston Smyposia
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Physiology in Press

First published online on June 7, 2002.
 Copyright © 2002 by The Physiological Society
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
542/3/699    most recent
2002.020677v1
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sochivko, D.
Right arrow Articles by Beck, H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Sochivko, D.
Right arrow Articles by Beck, H.

Received March 17, 2002
Accepted after revision May 15, 2002

The CaV2.3 Ca2+ channel subunit contributes to R-type Ca2+ currents in murine hippocampal and neocortical neurones

Dmitry Sochivko1, Alexey Pereverzev2, Neil Smyth3, Cornelia Gissel2, Toni Schneider{dagger}2, and Heinz Beck4*

1 Department of Epileptology, University of Bonn Medical Center, Sigmund-Freud Str. 25, 53105 Bonn, Germany
2 Department of Neurophysiology, University of Cologne, Robert-Koch-Str. 39, 50931 Cologne, Germany
3 Department of Biochemistry, University of Cologne, Joseph Stelzmann-Str. 52, 50931 Cologne, Germany
4 Department of Epileptology, University of Bonn Medical Center, Sigmund-Freud Str. 25, D-53105 Bonn, Germany

* To whom correspondence should be addressed. E-mail: heinz.beck{at}ukb.uni-bonn.de.

Different subtypes of voltage-dependent Ca2+ currents in native neurones are essential in coupling action potential firing to Ca2+ influx. For most of these currents, the underlying Ca2+ channel subunits have been identified on the basis of pharmacological and biophysical similarities. In contrast, the molecular basis of R-type Ca2+ currents remains controversial. We have therefore examined the contribution of the CaV2.3 ({alpha}1E) subunits to R-type currents in different types of central neurones using wild-type mice and mice in which the CaV2.3 subunit gene was deleted. In hippocampal CA1 pyramidal cells and dentate granule neurones, as well as neocortical neurones of wild-type mice, Ca2+ current components resistant to the combined application of {omega}-conotoxin GVIA and MVIIC, {omega}-agatoxin IVa and nifedipine (ICa,R) were detected (ICa,R) that were composed of a large R-type and a smaller T-type component. In CaV2.3-deficient mice, ICa,R was considerably reduced in CA1 neurones (79 %) and cortical neurones (87 %), with less reduction occurring in dentate granule neurones (47 %). Analysis of tail currents revealed that the reduction of ICa,R is due to a selective reduction of the rapidly deactivating R-type current component in CA1 and cortical neurones. In all cell types, ICa,R was highly sensitive to Ni2+ (100 µM: 71-86 % block). A selective antagonist of cloned CaV2.3 channels, the spider toxin SNX-482, partially inhibited ICa,R at concentrations up to 300 nM in dentate granule cells and cortical neurones (50 and 57 % block, EC50 30 and 47 nM, respectively). ICa,R in CA1 neurones was significantly less sensitive to SNX-482 (27 % block, 300 nM SNX-482). Taken together, our results show clearly that CaV2.3 subunits underlie a significant fraction of ICa,R in different types of central neurones. They also indicate that CaV2.3 subunits may give rise to Ca2+ currents with differing pharmacological properties in native neurones.




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
A. J. Becker, J. Pitsch, D. Sochivko, T. Opitz, M. Staniek, C.-C. Chen, K. P. Campbell, S. Schoch, Y. Yaari, and H. Beck
Transcriptional Upregulation of Cav3.2 Mediates Epileptogenesis in the Pilocarpine Model of Epilepsy
J. Neurosci., December 3, 2008; 28(49): 13341 - 13353.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
Y. Yaari, C. Yue, and H. Su
Recruitment of apical dendritic T-type Ca2+ channels by backpropagating spikes underlies de novo intrinsic bursting in hippocampal epileptogenesis
J. Physiol., April 15, 2007; 580(2): 435 - 450.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
U. Meza, A. Thapliyal, R. A. Bannister, and B. A. Adams
Neurokinin 1 Receptors Trigger Overlapping Stimulation and Inhibition of CaV2.3 (R-Type) Calcium Channels
Mol. Pharmacol., January 1, 2007; 71(1): 284 - 293.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Toro-Castillo, A. Thapliyal, H. Gonzalez-Ochoa, B. A. Adams, and U. Meza
Muscarinic modulation of Cav2.3 (R-type) calcium channels is antagonized by RGS3 and RGS3T
Am J Physiol Cell Physiol, January 1, 2007; 292(1): C573 - C580.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. Tai, J. B. Kuzmiski, and B. A. MacVicar
Muscarinic enhancement of R-type calcium currents in hippocampal CA1 pyramidal neurons.
J. Neurosci., June 7, 2006; 26(23): 6249 - 6258.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
Y.-J. Won, K. Whang, I. D. Kong, K.-S. Park, J.-W. Lee, and S.-W. Jeong
Expression Profiles of High Voltage-Activated Calcium Channels in Sympathetic and Parasympathetic Pelvic Ganglion Neurons Innervating the Urogenital System
J. Pharmacol. Exp. Ther., June 1, 2006; 317(3): 1064 - 1071.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. E. Metz, T. Jarsky, M. Martina, and N. Spruston
R-Type Calcium Channels Contribute to Afterdepolarization and Bursting in Hippocampal CA1 Pyramidal Neurons
J. Neurosci., June 15, 2005; 25(24): 5763 - 5773.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
X. Bian, X. Zhou, and J. J. Galligan
R-type calcium channels in myenteric neurons of guinea pig small intestine
Am J Physiol Gastrointest Liver Physiol, July 1, 2004; 287(1): G134 - G142.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
R. A. Bannister, K. Melliti, and B. A. Adams
Differential Modulation of CaV2.3 Ca2+ Channels by G{alpha}q/11-Coupled Muscarinic Receptors
Mol. Pharmacol., February 1, 2004; 65(2): 381 - 388.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Brandt, J. Striessnig, and T. Moser
CaV1.3 Channels Are Essential for Development and Presynaptic Activity of Cochlear Inner Hair Cells
J. Neurosci., November 26, 2003; 23(34): 10832 - 10840.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Kato, K. Ui-Tei, M. Watanabe, and Y. Sakuma
Characterization of Voltage-Gated Calcium Currents in Gonadotropin-Releasing Hormone Neurons Tagged with Green Fluorescent Protein in Rats
Endocrinology, November 1, 2003; 144(11): 5118 - 5125.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Breustedt, K. E. Vogt, R. J. Miller, R. A. Nicoll, and D. Schmitz
{alpha}1E-Containing Ca2+ channels are involved in synaptic plasticity
PNAS, October 14, 2003; 100(21): 12450 - 12455.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2002 The Physiological Society.