| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Received April 11, 2002
Accepted after revision September 2, 2002
1 Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, Nagoya City University, Mizuho-ku, Nagoya 467-8601, Japan
2 Department of Cellular and Molecular Pharmacology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Japan
3 Departments of Obstetrics and Gynecology and Cellular and Molecular Pharmacology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Japan
4 Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Japan
* To whom correspondence should be addressed. E-mail: og.yo{at}med.nagoya-cu.ac.jp.
It remains unclear in pre-eclampsia whether or not a functional change occurs in the role played by prostacyclin in endothelium-dependent relaxation in resistance arteries. We examined this using human omental resistance arteries (obtained from pre-eclamptic or normotensive pregnant women) in the presence of NG-nitro-L-arginine (L-NNA, an inhibitor of nitric oxide synthase). In endothelium-intact strips from both groups, 9,11-epithio-11,12-methano-thromboxane A2 (STA2, a thromboxane A2 mimetic) produced a contraction. Diclofenac (an inhibitor of cyclooxygenase) enhanced the STA2 contraction only in the normotensive pregnant group (1.4 times control, P < 0.01). In the presence of STA2, bradykinin (0.1 µM) produced an endothelium-dependent relaxation in both groups, the relaxation being significantly smaller for the pre-eclamptic group (P < 0.002). Diclofenac significantly attenuated the bradykinin-induced relaxation only for the normotensive pregnant group (31 % inhibition, P < 0.001). The bradykinin-induced membrane hyperpolarization consisted of diclofenac-sensitive and -insensitive components. The former, but not the latter, was significantly smaller in pre-eclampsia (-4.3 vs. -2.6 mV, P < 0.05). The concentrations of 6-keto-PGF1
(a stable metabolite of prostacyclin) in these arteries were significantly lower in pre-eclampsia in both the absence and presence of bradykinin (about 0.2-0.4 times the normotensive pregnant value in each case, P < 0.01). By contrast, both the relaxation and the membrane hyperpolarization in response to beraprost (10 nM, a stable analogue of prostacyclin) were similar between the two groups. We conclude that, in pre-eclampsia, a reduced part is played by prostaglandins in the endothelium-dependent relaxation seen in resistance arteries and that this may be due to a reduced production of prostacyclin by the endothelium.
This article has been cited by other articles:
|
|
 |
|
  L. Luksha, H. Nisell, N. Luksha, M. Kublickas, K. Hultenby, and K. Kublickiene Endothelium-derived hyperpolarizing factor in preeclampsia: heterogeneous contribution, mechanisms, and morphological prerequisites Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2008; 294(2): R510 - R519. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Kaaja and I. A. Greer Manifestations of Chronic Disease During Pregnancy JAMA, December 7, 2005; 294(21): 2751 - 2757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-L. Dong, K. E. Green, S. Vegiragu, G. D. V. Hankins, E. Martin, M. Chauhan, C. Thota, and C. Yallampalli Evidence for Decreased Calcitonin Gene-Related Peptide (CGRP) Receptors and Compromised Responsiveness to CGRP of Fetoplacental Vessels in Preeclamptic Pregnancies J. Clin. Endocrinol. Metab., April 1, 2005; 90(4): 2336 - 2343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Takeuchi, H. Watanabe, Q.-K. Tran, M. Ozeki, D. Sumi, T. Hayashi, A. Iguchi, L. J Ignarro, K. Ohashi, and H. Hayashi Nitric oxide: inhibitory effects on endothelial cell calcium signaling, prostaglandin I2 production and nitric oxide synthase expression Cardiovasc Res, April 1, 2004; 62(1): 194 - 201. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
