J Physiol Editor in Chief
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Physiology in Press

First published online on November 29, 2002.
 Copyright © 2002 by The Physiological Society
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
546/3/733    most recent
2002.028704v1
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, L.
Right arrow Articles by Sanderson, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, L.
Right arrow Articles by Sanderson, M. J.

Received July 18, 2002
Accepted after revision October 30, 2002

Oscillations in ciliary beat frequency and intracellular calcium concentration in rabbit tracheal epithelial cells induced by ATP

Luo Zhang1 and M. J. Sanderson2*

1 Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
2 Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA

* To whom correspondence should be addressed. E-mail: Michael.Sanderson{at}umassmed.edu.

To investigate how Ca2+ regulates airway ciliary activity, changes in ciliary beat frequency (CBF) and intracellular calcium concentration ([Ca2+]i) of rabbit tracheal ciliated cells in response to ATP, ciliated cells were simultaneously quantified with high-speed phase-contrast and fast fluorescence imaging. [ATP]<= 1 µM induced an increase in [Ca2+]i and CBF that declined to the initial basal levels and was followed by irregular brief increases in [Ca2+]i and CBF. [ATP] >1 but < 16 µM induced a similar increase in [Ca2+]i and CBF but this was followed by oscillations in CBF and [Ca2+]i. The minimum CBF of the oscillations in CBF remained elevated above the basal rate while the minimum concentration of the [Ca2+]i oscillations returned to the basal level. The minimum and maximum CBF of the oscillations in CBF were independent of the [ATP], whereas the frequency of the oscillations in CBF was dependent on the [ATP]. Similar oscillations in CBF and [Ca2+]i were induced by ATP- {gamma} -S. Although ADP, AMP and adenosine induced a Ca2+-independent increase in CBF, neither ATP nor ATP- {gamma} -S induced an increase in CBF when the Ca2+ increases were abolished by 20 µM BAPTA AM; a result suggesting that ATP hydrolysis was minimal. [ATP] >=16 µM induced a sustained elevation in CBF and only a temporary, non-oscillating increase in [Ca2+]i. A similar response was induced by thapsigargin (2 µM). Flash photolysis of caged Ca2+ (NP-EGTA) produced both transient and prolonged increases in [Ca2+]i which were accompanied by transient and sustained increases in CBF, respectively. From these results, we propose that CBF can be increased by a direct Ca2 -dependent mechanism that generates the rapid increases in CBF associated with the oscillations or by an indirect Ca2+-dependent mechanism that is responsible for the sustained minimum increase in CBF.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
I. M. Lorenzo, W. Liedtke, M. J. Sanderson, and M. A. Valverde
TRPV4 channel participates in receptor-operated calcium entry and ciliary beat frequency regulation in mouse airway epithelial cells
PNAS, August 26, 2008; 105(34): 12611 - 12616.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
K.-F. Lechtreck, P. Delmotte, M. L. Robinson, M. J. Sanderson, and G. B. Witman
Mutations in Hydin impair ciliary motility in mice
J. Cell Biol., February 6, 2008; 180(3): 633 - 643.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
B. Button, M. Picher, and R. C. Boucher
Differential effects of cyclic and constant stress on ATP release and mucociliary transport by human airway epithelia
J. Physiol., April 15, 2007; 580(2): 577 - 592.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. Nakahari
Regulation of ciliary beat frequency in airways: shear stress, ATP action, and its modulation
Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L612 - L613.
[Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Schmid, G. Bai, N. Schmid, M. Zaccolo, L. E. Ostrowski, G. E. Conner, N. Fregien, and M. Salathe
Real-time analysis of cAMP-mediated regulation of ciliary motility in single primary human airway epithelial cells
J. Cell Sci., October 15, 2006; 119(20): 4176 - 4186.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
P. Delmotte and M. J. Sanderson
Ciliary Beat Frequency Is Maintained at a Maximal Rate in the Small Airways of Mouse Lung Slices
Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 110 - 117.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Yang, J. Gao, M. Adamian, X.-H. Wen, B. Pawlyk, L. Zhang, M. J. Sanderson, J. Zuo, C. L. Makino, and T. Li
The Ciliary Rootlet Maintains Long-Term Stability of Sensory Cilia
Mol. Cell. Biol., May 15, 2005; 25(10): 4129 - 4137.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2002 The Physiological Society.