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Received August 1, 2002
Accepted after revision December 18, 2002
1 Department of Otolaryngology: HNS, Johns Hopkins University, 521 Traylor Building, 720 Rutland Avenue, Baltimore, MD 21205, USA
2 Department of Otolaryngology: Head and Neck Surgery, Johns Hopkins University, 521 Traylor Building, 720 Rutland Avenue, Baltimore, MD 21205, USA
* To whom correspondence should be addressed. E-mail: rkduncan{at}bme.jhu.edu.
The mechanism for electrical tuning in non-mammalian hair cells rests within the widely diverse kinetics of functionally distinct large conductance potassium (BK) channels, thought to result from alternative splicing of the pore-forming 
subunit and variable co-expression with an accessory 
subunit. Inside-out patches from hair cells along the chicken basilar papilla revealed 'tonotopic' gradations in calcium sensitivity and deactivation kinetics. The resonant frequency for the hair cell from which the patch was taken was estimated from deactivation rates, and this frequency reasonably matched that predicted from the originating cell's tonotopic location. The rates of deactivation for native BK channels were much faster than rates reported for cloned chicken BK channels including both and subunits. This result was surprising since patches were pulled from hair cells in the apical half of the papilla where subunits are most highly expressed. Heterogeneity in the properties of native chicken BK channels implies a high degree of molecular variation and hinders our ability to identify those molecular constituents.
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