| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Received October 10, 2002
Accepted after revision ,
1 Department of Clinical Physiology, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden
2 Exercise Metabolism Group, School of Medical Sciences, Faculty of Life Sciences, RMIT University, Melbourne, Victoria, Australia
3 Cellular Stress Group, Medical Research Council Clinical Sciences Centre, London, UK
4 Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden
5 Department of Clinical Physiology and Integrative Physiology, Karolinska Institutet, von Eulers väg 4, II, SE-171 77 Stockholm, Sweden
* To whom correspondence should be addressed. E-mail: Juleen.Zierath{at}fyfa.ki.se.
We determined whether mitogen-activated protein kinase (MAPK) and 5â-AMP-activated protein kinase (AMPK) signalling cascades are activated in response to intense exercise in skeletal muscle from six highly trained cyclists (peak O2 uptake (VO2,peak) 5.14 ± 0.1 l min-1) and four control subjects (VO2,peak 3.8 ± 0.1 l min-1) matched for age and body mass. Trained subjects completed eight 5 min bouts of cycling at ~85% of VO2,peak with 60 s recovery between work bouts. Control subjects performed four 5 min work bouts commencing at the same relative, but a lower absolute intensity, with a comparable rest interval. Vastus lateralis muscle biopsies were taken at rest and immediately after exercise. Extracellular regulated kinase (ERK1/2), p38 MAPK, histone H3, AMPK and ACC phosphorylation was determined by immunoblot analysis using phosphospecific antibodies. Activity of mitogen and stress-activated kinase 1 (MSK1; a substrate of ERK1/2 and p38 MAPK) and 
1 and 2 subunits of AMPK were determined by immune complex assay. ERK1/2 and p38 MAPK phosphorylation and MSK1 activity increased (P < 0.05) after exercise 2.6-, 2.1- and 2.0-fold, respectively, in control subjects and 1.5-, 1.6- and 1.4-fold, respectively, in trained subjects. Phosphorylation of histone H3, a substrate of MSK1, increased (P < 0.05) ~1.8-fold in both control and trained subject. AMPK2 activity increased (P < 0.05) after exercise 4.2- and 2.3-fold in control and trained subjects, respectively, whereas AMPK1 activity was not altered. Exercise increased ACC phosphorylation (P < 0.05) 1.9- and 2.8-fold in control and trained subjects. In conclusion, intense cycling exercise in subjects with a prolonged history of endurance training increases MAPK signalling to the downstream targets MSK1 and histone H3 and isoform-specific AMPK signalling to ACC. Importantly, exercise-induced signalling responses were greater in untrained men, even at the same relative exercise intensity, suggesting muscle from previously well-trained individuals requires a greater stimulus to activate signal transduction via these pathways.
This article has been cited by other articles:
|
|
 |
|
  K. A. Rasbach and R. G. Schnellmann Isoflavones Promote Mitochondrial Biogenesis J. Pharmacol. Exp. Ther., May 1, 2008; 325(2): 536 - 543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Roze, S. Betuing, C. Deyts, E. Marcon, K. Brami-Cherrier, C. Pages, S. Humbert, K. Merienne, and J. Caboche Mitogen- and stress-activated protein kinase-1 deficiency is involved in expanded-huntingtin-induced transcriptional dysregulation and striatal death FASEB J, April 1, 2008; 22(4): 1083 - 1093. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Thomson, C. A. Fick, and S. E. Gordon AMPK activation attenuates S6K1, 4E-BP1, and eEF2 signaling responses to high-frequency electrically stimulated skeletal muscle contractions J Appl Physiol, March 1, 2008; 104(3): 625 - 632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Aughey, K. T. Murphy, S. A. Clark, A. P. Garnham, R. J. Snow, D. Cameron-Smith, J. A. Hawley, and M. J. McKenna Muscle Na+-K+-ATPase activity and isoform adaptations to intense interval exercise and training in well-trained athletes J Appl Physiol, July 1, 2007; 103(1): 39 - 47. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. F. Kramer and L. J. Goodyear Exercise, MAPK, and NF-{kappa}B signaling in skeletal muscle J Appl Physiol, July 1, 2007; 103(1): 388 - 395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Freyssenet Energy sensing and regulation of gene expression in skeletal muscle J Appl Physiol, February 1, 2007; 102(2): 529 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Birk and J. F. P. Wojtaszewski Predominant {alpha}2/{beta}2/{gamma}3 AMPK activation during exercise in human skeletal muscle J. Physiol., December 15, 2006; 577(3): 1021 - 1032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Dreyer, S. Fujita, J. G. Cadenas, D. L. Chinkes, E. Volpi, and B. B. Rasmussen Resistance exercise increases AMPK activity and reduces 4E-BP1 phosphorylation and protein synthesis in human skeletal muscle J. Physiol., October 15, 2006; 576(2): 613 - 624. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Nadeau, L. B. Ehlers, L. E. Aguirre, R. L. Moore, K. N. Jew, H. K. Ortmeyer, B. C. Hansen, J. E. B. Reusch, and B. Draznin Exercise training and calorie restriction increase SREBP-1 expression and intramuscular triglyceride in skeletal muscle Am J Physiol Endocrinol Metab, July 1, 2006; 291(1): E90 - E98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hurst, E. B. Taylor, T. D. Cline, L. J. Greenwood, C. L. Compton, J. D. Lamb, and W. W. Winder AMP-activated protein kinase kinase activity and phosphorylation of AMP-activated protein kinase in contracting muscle of sedentary and endurance-trained rats Am J Physiol Endocrinol Metab, October 1, 2005; 289(4): E710 - E715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Creer, P. Gallagher, D. Slivka, B. Jemiolo, W. Fink, and S. Trappe Influence of muscle glycogen availability on ERK1/2 and Akt signaling after resistance exercise in human skeletal muscle J Appl Physiol, September 1, 2005; 99(3): 950 - 956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-C. Gomez-Cabrera, C. Borras, F. V Pallardo, J. Sastre, L. L. Ji, and J. Vina Decreasing xanthine oxidase-mediated oxidative stress prevents useful cellular adaptations to exercise in rats J. Physiol., August 15, 2005; 567(1): 113 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Taylor, D. Hurst, L. J. Greenwood, J. D. Lamb, T. D. Cline, S. N. Sudweeks, and W. W. Winder Endurance training increases LKB1 and MO25 protein but not AMP-activated protein kinase kinase activity in skeletal muscle Am J Physiol Endocrinol Metab, December 1, 2004; 287(6): E1082 - E1089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sakamoto, O. Goransson, D. G. Hardie, and D. R. Alessi Activity of LKB1 and AMPK-related kinases in skeletal muscle: effects of contraction, phenformin, and AICAR Am J Physiol Endocrinol Metab, August 1, 2004; 287(2): E310 - E317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. K. R. Karlsson, P.-A. Nilsson, J. Nilsson, A. V. Chibalin, J. R. Zierath, and E. Blomstrand Branched-chain amino acids increase p70S6k phosphorylation in human skeletal muscle after resistance exercise Am J Physiol Endocrinol Metab, July 1, 2004; 287(1): E1 - E7. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Leng, T. L. Steiler, and J. R. Zierath Effects of Insulin, Contraction, and Phorbol Esters on Mitogen-Activated Protein Kinase Signaling in Skeletal Muscle From Lean and ob/ob Mice Diabetes, June 1, 2004; 53(6): 1436 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Clark, Z.-P. Chen, K. T. Murphy, R. J. Aughey, M. J. McKenna, B. E. Kemp, and J. A. Hawley Intensified exercise training does not alter AMPK signaling in human skeletal muscle Am J Physiol Endocrinol Metab, May 1, 2004; 286(5): E737 - E743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. S. L. Thong, W. Derave, B. Urso, B. Kiens, and E. A. Richter Prior exercise increases basal and insulin-induced p38 mitogen-activated protein kinase phosphorylation in human skeletal muscle J Appl Physiol, June 1, 2003; 94(6): 2337 - 2341. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
