| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Received December 13, 2002
Accepted after revision March 18, 2003
1 Abteilung Zellphysiologie, Max-Planck-Institüt f ür medizinische Forschung, D-69120 Heidelberg, Germany,
2 Department of Physiology, New York Medical College, Valhalla, NY 10595, USA
3 Department of Molecular Neurobiology, University of Tokyo, Tokyo 108-8639, Japan
* To whom correspondence should be addressed. E-mail: ross{at}nymc.edu.
Calcium waves in layer 2/3 and layer 5 neocortical somatosensory pyramidal neurons were examined in slices from 2- to 8-week-old rats. Repetitive synaptic stimulation evoked a delayed, all-or-none [Ca2+]i increase primarily on the main dendritic shaft. This component was blocked by 1 mM MCPG, 10 µM ryanodine, 1 mg ml-1 internal heparin, and was not blocked by 400 µM internal Ruthenium Red, indicating that it was due to Ca2+ release from internal stores by inositol 1,4,5-trisphosphate (IP3) mobilized via activation of metabotropic glutamate receptors. Calcium waves were initiated on the apical shaft at sites between the soma to around the main branch point, mostly at insertion points of oblique dendrites, and spread in both directions along the shaft. In the proximal dendrites the peak amplitude of the resulting [Ca2+]i change was much larger than that evoked by a train of Na+ spikes. In distal dendrites the peak amplitude was comparable to the [Ca2+]i change due to a Ca2+ spike. IP3-mediated Ca2+ release also was observed in the presence of the metabotropic agonists t-ACPD and carbachol when backpropagating spikes were generated. Ca2+ entry through NMDA receptors was observed primarily on the oblique dendrites. The main differences between waves in neocortical neurons and in previously described hippocampal pyramidal neurons were, (a) Ca2+ waves in L5 neurons could be evoked further out along the main shaft, (b) Ca2+ waves extended slightly further out into the oblique dendrites and (c) higher concentrations of bath-applied t-ACPD and carbachol were required to generate Ca2+ release events by backpropagating action potentials.
This article has been cited by other articles:
|
|
 |
|
  K.-H. Cho, H.-J. Jang, E.-H. Lee, S. H. Yoon, S. J. Hahn, Y.-H. Jo, M.-S. Kim, and D.-J. Rhie Differential Cholinergic Modulation of Ca2+ Transients Evoked by Backpropagating Action Potentials in Apical and Basal Dendrites of Cortical Pyramidal Neurons J Neurophysiol, June 1, 2008; 99(6): 2833 - 2843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Mironov Metabotropic glutamate receptors activate dendritic calcium waves and TRPM channels which drive rhythmic respiratory patterns in mice J. Physiol., May 1, 2008; 586(9): 2277 - 2291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Larkum, S. Watanabe, N. Lasser-Ross, P. Rhodes, and W. N. Ross Dendritic Properties of Turtle Pyramidal Neurons J Neurophysiol, February 1, 2008; 99(2): 683 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Hagenston, J. S. Fitzpatrick, and M. F. Yeckel MGluR-Mediated Calcium Waves that Invade the Soma Regulate Firing in Layer V Medial Prefrontal Cortical Pyramidal Neurons Cereb Cortex, February 1, 2008; 18(2): 407 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Milojkovic, W.-L. Zhou, and S. D. Antic Voltage and calcium transients in basal dendrites of the rat prefrontal cortex J. Physiol., December 1, 2007; 585(2): 447 - 468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hong and W. N. Ross Priming of intracellular calcium stores in rat CA1 pyramidal neurons J. Physiol., October 1, 2007; 584(1): 75 - 87. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Power and P. Sah Distribution of IP3-mediated calcium responses and their role in nuclear signalling in rat basolateral amygdala neurons J. Physiol., May 1, 2007; 580(3): 835 - 857. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Cui, B. E. Bernier, M. T. Harnett, and H. Morikawa Differential Regulation of Action Potential- and Metabotropic Glutamate Receptor-Induced Ca2+ Signals by Inositol 1,4,5-Trisphosphate in Dopaminergic Neurons J. Neurosci., April 25, 2007; 27(17): 4776 - 4785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Billups, B. Billups, R. A. J. Challiss, and S. R. Nahorski Modulation of Gq-Protein-Coupled Inositol Trisphosphate and Ca2+ Signaling by the Membrane Potential J. Neurosci., September 27, 2006; 26(39): 9983 - 9995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Watanabe, M. Hong, N. Lasser-Ross, and W. N. Ross Modulation of calcium wave propagation in the dendrites and to the soma of rat hippocampal pyramidal neurons J. Physiol., September 1, 2006; 575(2): 455 - 468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Iyer, T. M. Hoogland, and P. Saggau Fast Functional Imaging of Single Neurons Using Random-Access Multiphoton (RAMP) Microscopy J Neurophysiol, January 1, 2006; 95(1): 535 - 545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Warrier, S. Borges, D. Dalcino, C. Walters, and M. Wilson Calcium From Internal Stores Triggers GABA Release From Retinal Amacrine Cells J Neurophysiol, December 1, 2005; 94(6): 4196 - 4208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Gulledge and G. J. Stuart Cholinergic Inhibition of Neocortical Pyramidal Neurons J. Neurosci., November 2, 2005; 25(44): 10308 - 10320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Jacob, C.-U. Choe, P. Uhlen, B. DeGray, M. F. Yeckel, and B. E. Ehrlich Signaling Microdomains Regulate Inositol 1,4,5-Trisphosphate-Mediated Intracellular Calcium Transients in Cultured Neurons J. Neurosci., March 16, 2005; 25(11): 2853 - 2864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Power and P. Sah Intracellular calcium store filling by an L-type calcium current in the basolateral amygdala at subthreshold membrane potentials J. Physiol., January 15, 2005; 562(2): 439 - 453. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Verkhratsky Physiology and Pathophysiology of the Calcium Store in the Endoplasmic Reticulum of Neurons Physiol Rev, January 1, 2005; 85(1): 201 - 279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Nash, J. M. Willets, B. Billups, R. A. John Challiss, and S. R. Nahorski Synaptic Activity Augments Muscarinic Acetylcholine Receptor-stimulated Inositol 1,4,5-Trisphosphate Production to Facilitate Ca2+ Release in Hippocampal Neurons J. Biol. Chem., November 19, 2004; 279(47): 49036 - 49044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Cai, C. Hisatsune, K. Nakamura, T. Nakamura, T. Inoue, and K. Mikoshiba Activity-dependent Expression of Inositol 1,4,5-Trisphosphate Receptor Type 1 in Hippocampal Neurons J. Biol. Chem., May 28, 2004; 279(22): 23691 - 23698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Birtoli and D. Ulrich Firing Mode-Dependent Synaptic Plasticity in Rat Neocortical Pyramidal Neurons J. Neurosci., May 26, 2004; 24(21): 4935 - 4940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-I. Yamada, H. Takechi, I. Kanchiku, T. Kita, and N. Kato Small-Conductance Ca2+-Dependent K+ Channels Are the Target of Spike-Induced Ca2+ Release in a Feedback Regulation of Pyramidal Cell Excitability J Neurophysiol, May 1, 2004; 91(5): 2322 - 2329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Connor, L. D. Pozzo-Miller, and B. Ross Synaptically driven Ca2+ release in hippocampal neurons J Neurophysiol, November 1, 2003; 90(5): 3579 - 3579. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
