J Physiol Editor in Chief
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Physiology in Press

First published online on June 6, 2003.
 Copyright © 2003 by The Physiological Society
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
550/3/739    most recent
2003.042127v2
2003.042127v1
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Djouhri, L.
Right arrow Articles by Lawson, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Djouhri, L.
Right arrow Articles by Lawson, S.

Received February 21, 2003
Accepted after revision May 2, 2003

The TTX-resistant sodium channel Nav1.8 (SNS/PN3): expression and correlation with membrane properties in rat nociceptive primary afferent neurons

Laiche Djouhri1, Xing Fang1, Kengi Okuse2, John N. Wood2, Carol M. Berry1, and S. Lawson3*

1 Department of Physiology, University of Bristol Medical School, Bristol BS8 1TD, UK
2 Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
3 Department of Physiology, University of Bristol, Medical School, University Walk, Bristol BS8 1TD, UK

* To whom correspondence should be addressed. E-mail: sally.lawson{at}bristol.ac.uk.

We have examined the distribution of the sensory neuron-specific Na+ channel Nav1.8 (SNS/PN3) in nociceptive and non-nociceptive dorsal root ganglion (DRG) neurons and whether its distribution is related to neuronal membrane properties. Nav1.8-like immunoreactivity (Nav1.8-LI) was examined with an affinity purified polyclonal antiserum (SNS11) in rat DRG neurons that were classified according to sensory receptive properties and by conduction velocity (CV) as C-, A{delta}- or A{alpha}/{beta}. A significantly higher proportion of nociceptive than low threshold mechanoreceptive (LTM) neurons showed Nav1.8-LI, and nociceptive neurons had significantly more intense immunoreactivity in their somata than LTM neurons. Results showed that 89, 93 and 60 % of C-, A{delta}- and A{alpha}/{beta}-fibre nociceptive units respectively and 88 % of C-unresponsive units were positive. C-unresponsive units had electrical membrane properties similar to C-nociceptors and were considered to be nociceptive-type neurons. Weak positive Nav1.8-LI was also present in some LTM units including a C LTM, all A{delta} LTM units (D hair), about 10 % of cutaneous LTM A{alpha}/{beta}-units, but no muscle spindle afferent units. Nav1.8-LI intensity was negatively correlated with soma size (all neurons) and with dorsal root CVs in A- but not C-fibre neurons. Nav1.8-LI intensity was positively correlated with action potential (AP) duration (both rise and fall time) in A-fibre neurons and with AP rise time only in positive C-fibre neurons. It was also positively correlated with AP overshoot in positive neurons. Thus high levels of Nav1.8 protein may contribute to the longer AP durations (especially in A-fibre neurons) and larger AP overshoots that are typical of nociceptors.




This article has been cited by other articles:


Home page
 Anesth. Analg.Home page
C.-F. Wang, P. Gerner, B. Schmidt, Z. Z. Xu, C. Nau, S.-Y. Wang, R.-R. Ji, and G. K. Wang
Use of Bulleyaconitine A as an Adjuvant for Prolonged Cutaneous Analgesia in the Rat
Anesth. Analg., October 1, 2008; 107(4): 1397 - 1405.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
G. Dussor, M. J. Zylka, D. J. Anderson, and E. W. McCleskey
Cutaneous Sensory Neurons Expressing the Mrgprd Receptor Sense Extracellular ATP and Are Putative Nociceptors
J Neurophysiol, April 1, 2008; 99(4): 1581 - 1589.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Hudmon, J.-S. Choi, L. Tyrrell, J. A. Black, A. M. Rush, S. G. Waxman, and S. D. Dib-Hajj
Phosphorylation of Sodium Channel Nav1.8 by p38 Mitogen-Activated Protein Kinase Increases Current Density in Dorsal Root Ganglion Neurons
J. Neurosci., March 19, 2008; 28(12): 3190 - 3201.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. McGaraughty, K. L. Chu, M. J. C. Scanio, M. E. Kort, C. R. Faltynek, and M. F. Jarvis
A Selective Nav1.8 Sodium Channel Blocker, A-803467 [5-(4-Chlorophenyl-N-(3,5-dimethoxyphenyl)furan-2-carboxamide], Attenuates Spinal Neuronal Activity in Neuropathic Rats
J. Pharmacol. Exp. Ther., March 1, 2008; 324(3): 1204 - 1211.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
R. De Col, K. Messlinger, and R. W. Carr
Conduction velocity is regulated by sodium channel inactivation in unmyelinated axons innervating the rat cranial meninges
J. Physiol., February 15, 2008; 586(4): 1089 - 1103.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
V. Pinto, V. A. Derkach, and B. V. Safronov
Role of TTX-Sensitive and TTX-Resistant Sodium Channels in A{delta}- and C-Fiber Conduction and Synaptic Transmission
J Neurophysiol, February 1, 2008; 99(2): 617 - 628.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. M. Rush, T. R. Cummins, and S. G. Waxman
Multiple sodium channels and their roles in electrogenesis within dorsal root ganglion neurons
J. Physiol., February 15, 2007; 579(1): 1 - 14.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J.-S. Choi, S. D. Dib-Hajj, and S. G. Waxman
Differential Slow Inactivation and Use-Dependent Inhibition of Nav1.8 Channels Contribute to Distinct Firing Properties in IB4+ and IB4- DRG Neurons
J Neurophysiol, February 1, 2007; 97(2): 1258 - 1265.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
A. Leffler, A. Reiprich, D. P. Mohapatra, and C. Nau
Use-Dependent Block by Lidocaine but Not Amitriptyline Is More Pronounced in Tetrodotoxin (TTX)-Resistant Nav1.8 Than in TTX-Sensitive Na+ Channels
J. Pharmacol. Exp. Ther., January 1, 2007; 320(1): 354 - 364.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Ekberg, A. Jayamanne, C. W. Vaughan, S. Aslan, L. Thomas, J. Mould, R. Drinkwater, M. D. Baker, B. Abrahamsen, J. N. Wood, et al.
{micro}O-conotoxin MrVIB selectively blocks Nav1.8 sensory neuron specific sodium channels and chronic pain behavior without motor deficits
PNAS, November 7, 2006; 103(45): 17030 - 17035.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. Foulkes, M. A. Nassar, T. Lane, E. A. Matthews, M. D. Baker, V. Gerke, K. Okuse, A. H. Dickenson, and J. N. Wood
Deletion of Annexin 2 Light Chain p11 in Nociceptors Causes Deficits in Somatosensory Coding and Pain Behavior
J. Neurosci., October 11, 2006; 26(41): 10499 - 10507.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
K. Hillsley, J.-H. Lin, A. Stanisz, D. Grundy, J. Aerssens, P. J. Peeters, D. Moechars, B. Coulie, and R. H. Stead
Dissecting the role of sodium currents in visceral sensory neurons in a model of chronic hyperexcitability using Nav1.8 and Nav1.9 null mice
J. Physiol., October 1, 2006; 576(1): 257 - 267.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
X. Fang, L. Djouhri, S. McMullan, C. Berry, S. G. Waxman, K. Okuse, and S. N. Lawson
Intense isolectin-B4 binding in rat dorsal root ganglion neurons distinguishes C-fiber nociceptors with broad action potentials and high Nav1.9 expression.
J. Neurosci., July 5, 2006; 26(27): 7281 - 7292.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J.-S. Choi, A. Hudmon, S. G. Waxman, and S. D. Dib-Hajj
Calmodulin Regulates Current Density and Frequency-Dependent Inhibition of Sodium Channel Nav1.8 in DRG Neurons
J Neurophysiol, July 1, 2006; 96(1): 97 - 108.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. M. Rush, S. D. Dib-Hajj, S. Liu, T. R. Cummins, J. A. Black, and S. G. Waxman
A single sodium channel mutation produces hyper- or hypoexcitability in different types of neurons
PNAS, May 23, 2006; 103(21): 8245 - 8250.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
P. J. Peeters, J. Aerssens, R. de Hoogt, A. Stanisz, H. W. Gohlmann, K. Hillsley, A. Meulemans, D. Grundy, R. H. Stead, and B. Coulie
Molecular profiling of murine sensory neurons in the nodose and dorsal root ganglia labeled from the peritoneal cavity
Physiol Genomics, February 23, 2006; 24(3): 252 - 263.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
X Fang, S McMullan, S. N Lawson, and L Djouhri
Electrophysiological differences between nociceptive and non-nociceptive dorsal root ganglion neurones in the rat in vivo
J. Physiol., June 15, 2005; 565(3): 927 - 943.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
X. Fang, L. Djouhri, S. McMullan, C. Berry, K. Okuse, S. G. Waxman, and S. N. Lawson
trkA Is Expressed in Nociceptive Neurons and Influences Electrophysiological Properties via Nav1.8 Expression in Rapidly Conducting Nociceptors
J. Neurosci., May 11, 2005; 25(19): 4868 - 4878.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. J. Devaux and S. S. Scherer
Altered Ion Channels in an Animal Model of Charcot-Marie-Tooth Disease Type IA
J. Neurosci., February 9, 2005; 25(6): 1470 - 1480.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. R. Cummins, S. D. Dib-Hajj, and S. G. Waxman
Electrophysiological Properties of Mutant Nav1.7 Sodium Channels in a Painful Inherited Neuropathy
J. Neurosci., September 22, 2004; 24(38): 8232 - 8236.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. A. Nassar, L. C. Stirling, G. Forlani, M. D. Baker, E. A. Matthews, A. H. Dickenson, and J. N. Wood
Nociceptor-specific gene deletion reveals a major role for Nav1.7 (PN1) in acute and inflammatory pain
PNAS, August 24, 2004; 101(34): 12706 - 12711.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
E. K. Wittmack, A. M. Rush, M. J. Craner, M. Goldfarb, S. G. Waxman, and S. D. Dib-Hajj
Fibroblast Growth Factor Homologous Factor 2B: Association with Nav1.6 and Selective Colocalization at Nodes of Ranvier of Dorsal Root Axons
J. Neurosci., July 28, 2004; 24(30): 6765 - 6775.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2003 The Physiological Society.