| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Received February 21, 2003
Accepted after revision May 2, 2003
1 Department of Physiology, University of Bristol Medical School, Bristol BS8 1TD, UK
2 Department of Biology, University College London, Gower Street, London WC1E 6BT, UK
3 Department of Physiology, University of Bristol, Medical School, University Walk, Bristol BS8 1TD, UK
* To whom correspondence should be addressed. E-mail: sally.lawson{at}bristol.ac.uk.
We have examined the distribution of the sensory neuron-specific Na+ channel Nav1.8 (SNS/PN3) in nociceptive and non-nociceptive dorsal root ganglion (DRG) neurons and whether its distribution is related to neuronal membrane properties. Nav1.8-like immunoreactivity (Nav1.8-LI) was examined with an affinity purified polyclonal antiserum (SNS11) in rat DRG neurons that were classified according to sensory receptive properties and by conduction velocity (CV) as C-, Ad[%%%]- or A
/
. A significantly higher proportion of nociceptive than low threshold mechanoreceptive (LTM) neurons showed Nav1.8-LI, and nociceptive neurons had significantly more intense immunoreactivity in their somata than LTM neurons. Results showed that 89, 93 and 60 % of C-, Ad[%%%]- and A/-fibre nociceptive units respectively and 88 % of C-unresponsive units were positive. C-unresponsive units had electrical membrane properties similar to C-nociceptors and were considered to be nociceptive-type neurons. Weak positive Nav1.8-LI was also present in some LTM units including a C LTM, all Ad[%%%] LTM units (D hair), about 10 % of cutaneous LTM A/-units, but no muscle spindle afferent units. Nav1.8-LI intensity was negatively correlated with soma size (all neurons) and with dorsal root CVs in A- but not C-fibre neurons. Nav1.8-LI intensity was positively correlated with action potential (AP) duration (both rise and fall time) in A-fibre neurons and with AP rise time only in positive C-fibre neurons. It was also positively correlated with AP overshoot in positive neurons. Thus high levels of Nav1.8 protein may contribute to the longer AP durations (especially in A-fibre neurons) and larger AP overshoots that are typical of nociceptors.
This article has been cited by other articles:
|
|
 |
|
  C.-F. Wang, P. Gerner, B. Schmidt, Z. Z. Xu, C. Nau, S.-Y. Wang, R.-R. Ji, and G. K. Wang Use of Bulleyaconitine A as an Adjuvant for Prolonged Cutaneous Analgesia in the Rat Anesth. Analg., October 1, 2008; 107(4): 1397 - 1405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Dussor, M. J. Zylka, D. J. Anderson, and E. W. McCleskey Cutaneous Sensory Neurons Expressing the Mrgprd Receptor Sense Extracellular ATP and Are Putative Nociceptors J Neurophysiol, April 1, 2008; 99(4): 1581 - 1589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hudmon, J.-S. Choi, L. Tyrrell, J. A. Black, A. M. Rush, S. G. Waxman, and S. D. Dib-Hajj Phosphorylation of Sodium Channel Nav1.8 by p38 Mitogen-Activated Protein Kinase Increases Current Density in Dorsal Root Ganglion Neurons J. Neurosci., March 19, 2008; 28(12): 3190 - 3201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. McGaraughty, K. L. Chu, M. J. C. Scanio, M. E. Kort, C. R. Faltynek, and M. F. Jarvis A Selective Nav1.8 Sodium Channel Blocker, A-803467 [5-(4-Chlorophenyl-N-(3,5-dimethoxyphenyl)furan-2-carboxamide], Attenuates Spinal Neuronal Activity in Neuropathic Rats J. Pharmacol. Exp. Ther., March 1, 2008; 324(3): 1204 - 1211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. De Col, K. Messlinger, and R. W. Carr Conduction velocity is regulated by sodium channel inactivation in unmyelinated axons innervating the rat cranial meninges J. Physiol., February 15, 2008; 586(4): 1089 - 1103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Pinto, V. A. Derkach, and B. V. Safronov Role of TTX-Sensitive and TTX-Resistant Sodium Channels in A{delta}- and C-Fiber Conduction and Synaptic Transmission J Neurophysiol, February 1, 2008; 99(2): 617 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Rush, T. R. Cummins, and S. G. Waxman Multiple sodium channels and their roles in electrogenesis within dorsal root ganglion neurons J. Physiol., February 15, 2007; 579(1): 1 - 14. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-S. Choi, S. D. Dib-Hajj, and S. G. Waxman Differential Slow Inactivation and Use-Dependent Inhibition of Nav1.8 Channels Contribute to Distinct Firing Properties in IB4+ and IB4- DRG Neurons J Neurophysiol, February 1, 2007; 97(2): 1258 - 1265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Leffler, A. Reiprich, D. P. Mohapatra, and C. Nau Use-Dependent Block by Lidocaine but Not Amitriptyline Is More Pronounced in Tetrodotoxin (TTX)-Resistant Nav1.8 Than in TTX-Sensitive Na+ Channels J. Pharmacol. Exp. Ther., January 1, 2007; 320(1): 354 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ekberg, A. Jayamanne, C. W. Vaughan, S. Aslan, L. Thomas, J. Mould, R. Drinkwater, M. D. Baker, B. Abrahamsen, J. N. Wood, et al. {micro}O-conotoxin MrVIB selectively blocks Nav1.8 sensory neuron specific sodium channels and chronic pain behavior without motor deficits PNAS, November 7, 2006; 103(45): 17030 - 17035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Foulkes, M. A. Nassar, T. Lane, E. A. Matthews, M. D. Baker, V. Gerke, K. Okuse, A. H. Dickenson, and J. N. Wood Deletion of Annexin 2 Light Chain p11 in Nociceptors Causes Deficits in Somatosensory Coding and Pain Behavior J. Neurosci., October 11, 2006; 26(41): 10499 - 10507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hillsley, J.-H. Lin, A. Stanisz, D. Grundy, J. Aerssens, P. J. Peeters, D. Moechars, B. Coulie, and R. H. Stead Dissecting the role of sodium currents in visceral sensory neurons in a model of chronic hyperexcitability using Nav1.8 and Nav1.9 null mice J. Physiol., October 1, 2006; 576(1): 257 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Fang, L. Djouhri, S. McMullan, C. Berry, S. G. Waxman, K. Okuse, and S. N. Lawson Intense isolectin-B4 binding in rat dorsal root ganglion neurons distinguishes C-fiber nociceptors with broad action potentials and high Nav1.9 expression. J. Neurosci., July 5, 2006; 26(27): 7281 - 7292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-S. Choi, A. Hudmon, S. G. Waxman, and S. D. Dib-Hajj Calmodulin Regulates Current Density and Frequency-Dependent Inhibition of Sodium Channel Nav1.8 in DRG Neurons J Neurophysiol, July 1, 2006; 96(1): 97 - 108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Rush, S. D. Dib-Hajj, S. Liu, T. R. Cummins, J. A. Black, and S. G. Waxman A single sodium channel mutation produces hyper- or hypoexcitability in different types of neurons PNAS, May 23, 2006; 103(21): 8245 - 8250. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Peeters, J. Aerssens, R. de Hoogt, A. Stanisz, H. W. Gohlmann, K. Hillsley, A. Meulemans, D. Grundy, R. H. Stead, and B. Coulie Molecular profiling of murine sensory neurons in the nodose and dorsal root ganglia labeled from the peritoneal cavity Physiol Genomics, February 23, 2006; 24(3): 252 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X Fang, S McMullan, S. N Lawson, and L Djouhri Electrophysiological differences between nociceptive and non-nociceptive dorsal root ganglion neurones in the rat in vivo J. Physiol., June 15, 2005; 565(3): 927 - 943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Fang, L. Djouhri, S. McMullan, C. Berry, K. Okuse, S. G. Waxman, and S. N. Lawson trkA Is Expressed in Nociceptive Neurons and Influences Electrophysiological Properties via Nav1.8 Expression in Rapidly Conducting Nociceptors J. Neurosci., May 11, 2005; 25(19): 4868 - 4878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Devaux and S. S. Scherer Altered Ion Channels in an Animal Model of Charcot-Marie-Tooth Disease Type IA J. Neurosci., February 9, 2005; 25(6): 1470 - 1480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Cummins, S. D. Dib-Hajj, and S. G. Waxman Electrophysiological Properties of Mutant Nav1.7 Sodium Channels in a Painful Inherited Neuropathy J. Neurosci., September 22, 2004; 24(38): 8232 - 8236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Nassar, L. C. Stirling, G. Forlani, M. D. Baker, E. A. Matthews, A. H. Dickenson, and J. N. Wood Nociceptor-specific gene deletion reveals a major role for Nav1.7 (PN1) in acute and inflammatory pain PNAS, August 24, 2004; 101(34): 12706 - 12711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. K. Wittmack, A. M. Rush, M. J. Craner, M. Goldfarb, S. G. Waxman, and S. D. Dib-Hajj Fibroblast Growth Factor Homologous Factor 2B: Association with Nav1.6 and Selective Colocalization at Nodes of Ranvier of Dorsal Root Axons J. Neurosci., July 28, 2004; 24(30): 6765 - 6775. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
