HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 538/2/383    most recent 2001.013397v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, S. Articles by Stanley, E. F. Search for Related Content PubMed PubMed Citation Articles by Chen, S. Articles by Stanley, E. F.

Enhancement of presynaptic calcium current by cysteine string protein

Shan Chen*, Xu Zheng*, Karen L. Schulze †, Terry Morris ‡, Hugo Bellen † and Elis F. Stanley *‡

The isolated chick ciliary neuron calyx synapse preparation was used to test cysteine string protein (CSP) action on presynaptic N-type Ca²⁺ channels. Endogenous CSP was localized primarily to secretory vesicle clusters in the presynaptic nerve terminal. Introduction of recombinant CSP into the voltage clamped terminal resulted in a prominent increase in Ca²⁺ current amplitude. However, this increase could not be attributed to a change in Ca²⁺ channel kinetics, voltage dependence, prepulse inactivation, or G protein inhibition but was attributed to the recruitment of dormant channels. Secretory vesicle associated endogenous CSP may play an important role in enhancing Ca²⁺ channel activity at the transmitter release site.

This article has been cited by other articles:

				G. B. Awatramani, J. D. Boyd, K. R. Delaney, and T. H. Murphy Effective release rates at single rat Schaffer collateral-CA1 synapses during sustained theta-burst activity revealed by optical imaging J. Physiol., July 15, 2007; 582(2): 583 - 595. [Abstract] [Full Text] [PDF]

				G. B. Smith, J. A. Umbach, A. Hirano, and C. B. Gundersen Interaction between Constitutively Expressed Heat Shock Protein, Hsc 70, and Cysteine String Protein Is Important for Cortical Granule Exocytosis in Xenopus Oocytes J. Biol. Chem., September 23, 2005; 280(38): 32669 - 32675. [Abstract] [Full Text] [PDF]

				M. Natochin, T. N. Campbell, B. Barren, L. C. Miller, S. Hameed, N. O. Artemyev, and J. E. A. Braun Characterization of the G{alpha}s Regulator Cysteine String Protein J. Biol. Chem., August 26, 2005; 280(34): 30236 - 30241. [Abstract] [Full Text] [PDF]

				P. Bronk, Z. Nie, M. K. Klose, K. Dawson-Scully, J. Zhang, R. M. Robertson, H. L. Atwood, and K. E. Zinsmaier The Multiple Functions of Cysteine-String Protein Analyzed at Drosophila Nerve Terminals J. Neurosci., March 2, 2005; 25(9): 2204 - 2214. [Abstract] [Full Text] [PDF]

				Q. Li, A. Lau, T. J. Morris, L. Guo, C. B. Fordyce, and E. F. Stanley A Syntaxin 1, G{alpha}o, and N-Type Calcium Channel Complex at a Presynaptic Nerve Terminal: Analysis by Quantitative Immunocolocalization J. Neurosci., April 21, 2004; 24(16): 4070 - 4081. [Abstract] [Full Text] [PDF]

				L. C. Miller, L. A. Swayne, L. Chen, Z.-P. Feng, J. L. Wacker, P. J. Muchowski, G. W. Zamponi, and J. E. A. Braun Cysteine String Protein (CSP) Inhibition of N-type Calcium Channels Is Blocked by Mutant Huntingtin J. Biol. Chem., December 26, 2003; 278(52): 53072 - 53081. [Abstract] [Full Text] [PDF]

				L. C. Miller, L. A. Swayne, J. G. Kay, Z.-P. Feng, S. E. Jarvis, G. W. Zamponi, and J. E. A. Braun Molecular determinants of cysteine string protein modulation of N-type calcium channels J. Cell Sci., July 15, 2003; 116(14): 2967 - 2974. [Abstract] [Full Text] [PDF]

				C. Andriamampandry, C. Muller, C. Schmidt-Mutter, S. Gobaille, M. Spedding, D. Aunis, and M. Maitre Mss4 Gene Is Up-Regulated in Rat Brain after Chronic Treatment with Antidepressant and Down-Regulated When Rats Are Anhedonic Mol. Pharmacol., December 1, 2002; 62(6): 1332 - 1338. [Abstract] [Full Text] [PDF]