J Physiol Society Meetings
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Physiol Volume 541, Number 1, 139-158, May 15, 2002 DOI: 10.1113/jphysiol.2001.013267
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
541/1/139    most recent
2001.013267v1
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vaughan-Jones, R. D.
Right arrow Articles by Spitzer, K. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vaughan-Jones, R. D.
Right arrow Articles by Spitzer, K. W.
Journal of Physiology (2002), 541.1, pp. 139-158
© Copyright 2002 The Physiological Society
DOI: 10.1113/jphysiol.2001.013267

Intrinsic H+ ion mobility in the rabbit ventricular myocyte

R. D. Vaughan-Jones *, B. E. Peercy †, J. P. Keener † and K. W. Spitzer ‡

* University Laboratory of Physiology, Parks Road, Oxford OX1 3PT, UK, ‡ Nora Eccles Harrison Cardiovascular Research and Training Institute and † Department of Mathematics, University of Utah, Salt Lake City, UT, USA

The intrinsic mobility of intracellular H+ ions was investigated by confocally imaging the longitudinal movement of acid inside rabbit ventricular myocytes loaded with the acetoxymethyl ester (AM) form of carboxy-seminaphthorhodafluor-1 (carboxy-SNARF-1). Acid was diffused into one end of the cell through a patch pipette filled with an isotonic KCl solution of pH 3.0. Intracellular H+ mobility was low, acid taking 20-30 s to move 40 µm down the cell. Inhibiting sarcolemmal Na+-H+ exchange with 1 mM amiloride had no effect on this time delay. Net H+i movement was associated with a longitudinal intracellular pH (pHi) gradient of up to 0.4 pH units. H+i movement could be modelled using the equations for diffusion, assuming an apparent diffusion coefficient for H+ ions (DHapp) of 3.78 times 10-7 cm2 s-1, a value more than 300-fold lower than the H+ diffusion coefficient in a dilute, unbuffered solution. Measurement of the intracellular concentration of SNARF (~400 µM) and its intracellular diffusion coefficient (0.9 times 10-7 cm2 s-1) indicated that the fluorophore itself exerted an insignificant effect (between 0.6 and 3.3 %) on the longitudinal movement of H+ equivalents inside the cell. The longitudinal movement of intracellular H+ is discussed in terms of a diffusive shuttling of H+ equivalents on high capacity mobile buffers which comprise about half (~11 mM) of the total intrinsic buffering capacity within the myocyte (the other half being fixed buffer sites on low mobility, intracellular proteins). Intrinsic H+i mobility is consistent with an average diffusion coefficient for the intracellular mobile buffers (Dmob) of ~9 times 10-7 cm2 s-1.



This article has been cited by other articles:


Home page
 J. Physiol.Home page
T. E. DeCoursey
Voltage-gated proton channels: what's next?
J. Physiol., November 15, 2008; 586(22): 5305 - 5324.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. M. Becker and J. W. Deitmer
Nonenzymatic Proton Handling by Carbonic Anhydrase II during H+-Lactate Cotransport via Monocarboxylate Transporter 1
J. Biol. Chem., August 1, 2008; 283(31): 21655 - 21667.
[Abstract] [Full Text] [PDF]

Home page
 PerfusionHome page
J. R Triana, M. Yanagihashi, and D. F Larson
Mathematical modeling of buffers used in myocardial preservation
Perfusion, September 1, 2007; 22(5): 353 - 362.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M.-H. T. Nguyen, S. J. Dudycha, and M. S. Jafri
Effect of Ca2+ on cardiac mitochondrial energy production is modulated by Na+ and H+ dynamics
Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2004 - C2020.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
L. F. Barros and C. Martinez
An Enquiry into Metabolite Domains
Biophys. J., June 1, 2007; 92(11): 3878 - 3884.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
P. Swietach, A. Rossini, K. W. Spitzer, and R. D. Vaughan-Jones
H+ Ion Activation and Inactivation of the Ventricular Gap Junction: A Basis for Spatial Regulation of Intracellular pH
Circ. Res., April 13, 2007; 100(7): 1045 - 1054.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
P. Swietach, K. W. Spitzer, and R. D. Vaughan-Jones
pH-Dependence of Extrinsic and Intrinsic H+-Ion Mobility in the Rat Ventricular Myocyte, Investigated Using Flash Photolysis of a Caged-H+ Compound
Biophys. J., January 15, 2007; 92(2): 641 - 653.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
E. J. Crampin and N. P. Smith
A Dynamic Model of Excitation-Contraction Coupling during Acidosis in Cardiac Ventricular Myocytes
Biophys. J., May 1, 2006; 90(9): 3074 - 3090.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. M. Becker, D. Hirnet, C. Fecher-Trost, D. Sultemeyer, and J. W. Deitmer
Transport Activity of MCT1 Expressed in Xenopus Oocytes Is Increased by Interaction with Carbonic Anhydrase
J. Biol. Chem., December 2, 2005; 280(48): 39882 - 39889.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
P. Hunter and P. Nielsen
A Strategy for Integrative Computational Physiology
Physiology, October 1, 2005; 20(5): 316 - 325.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
P. Swietach and R. D Vaughan-Jones
Relationship between intracellular pH and proton mobility in rat and guinea-pig ventricular myocytes
J. Physiol., August 1, 2005; 566(3): 793 - 806.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
P. Swietach, C.-H. Leem, K. W. Spitzer, and R. D. Vaughan-Jones
Experimental Generation and Computational Modeling of Intracellular pH Gradients in Cardiac Myocytes
Biophys. J., April 1, 2005; 88(4): 3018 - 3037.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P. Swietach and R. D. Vaughan-Jones
Novel method for measuring junctional proton permeation in isolated ventricular myocyte cell pairs
Am J Physiol Heart Circ Physiol, November 1, 2004; 287(5): H2352 - H2363.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H.-a. Ro and J. H. Carson
pH Microdomains in Oligodendrocytes
J. Biol. Chem., August 27, 2004; 279(35): 37115 - 37123.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
F. B. Loiselle, P. E. Morgan, B. V. Alvarez, and J. R. Casey
Regulation of the human NBC3 Na+/HCO3- cotransporter by carbonic anhydrase II and PKA
Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1423 - C1433.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
M. Karmazyn, Q. Liu, X. T. Gan, B. J. Brix, and L. Fliegel
Aldosterone Increases NHE-1 Expression and Induces NHE-1-Dependent Hypertrophy in Neonatal Rat Ventricular Myocytes
Hypertension, December 1, 2003; 42(6): 1171 - 1176.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. Zaniboni, A. Rossini, P. Swietach, N. Banger, K. W. Spitzer, and R. D. Vaughan-Jones
Proton Permeation Through the Myocardial Gap Junction
Circ. Res., October 17, 2003; 93(8): 726 - 735.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Zaniboni, P. Swietach, A. Rossini, T. Yamamoto, K. W. Spitzer, and R. D. Vaughan-Jones
Intracellular proton mobility and buffering power in cardiac ventricular myocytes from rat, rabbit, and guinea pig
Am J Physiol Heart Circ Physiol, August 7, 2003; 285(3): H1236 - H1246.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 The Physiological Society.