HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 544/3/715    most recent 2002.029736v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sudweeks, S. N. Articles by Yakel, J. L. Search for Related Content PubMed PubMed Citation Articles by Sudweeks, S. N. Articles by Yakel, J. L.

Serotonin 5-HT₃ receptors in rat CA1 hippocampal interneurons: functional and molecular characterization

Sterling N. Sudweeks, Johannes A. van Hooft* and Jerrel L. Yakel

The molecular makeup of the serotonin 5-HT₃ receptor (5-HT₃R) channel was investigated in rat hippocampal CA1 interneurons in slices using single-cell RT-PCR and patch-clamp recording techniques. We tested for the expression of the 5-HT_3A (both short and long splice variants) and 5-HT_3B subunits, as well as the expression of the 4 subunit of the neuronal nicotinic ACh receptors (nAChRs), the latter of which has been shown to co-assemble with the 5-HT_3A subunit in heterologous expression systems. Both the 5-HT_3A-short and 4-nAChR subunits were expressed in these interneurons, but we could not detect any expression of either the 5-HT_3B or the 5-HT_3A-long subunits. Furthermore, there was a strong tendency for the 5-HT_3A-short and 4-nAChR subunits to be co-expressed in individual interneurons. To assess whether there was any functional evidence for co-assembly between the 5-HT_3A-short and 4-nAChR subunits, we used the sulphydryl agent 2-aminoethyl methanethiosulphonate (MTSEA), which has previously been shown to modulate expressed 5-HT₃Rs that contain the 4-nAChR subunit. In half of the interneurons examined, MTSEA significantly enhanced the amplitude of the 5-HT₃R-mediated responses, which is consistent with the notion that the 4-nAChR subunit co-assembles with the 5-HT_3A subunit to form a native heteromeric 5-HT₃R channel in rat CA1 hippocampal interneurons in vivo. In addition, the single-channel properties of the 5-HT₃R were investigated in outside-out patches. No resolvable single-channel currents were observed. Using non-stationary fluctuation analysis, we obtained an estimate of the single-channel conductance of 4 pS, which is well below that expected for channels containing both the 5-HT_3A and 5-HT_3B subunits.

This article has been cited by other articles:

				L. Khiroug, R. Giniatullin, R. C. Klein, D. Fayuk, and J. L. Yakel Functional Mapping and Ca2+ Regulation of Nicotinic Acetylcholine Receptor Channels in Rat Hippocampal CA1 Neurons J. Neurosci., October 8, 2003; 23(27): 9024 - 9031. [Abstract] [Full Text] [PDF]

				J. A. van Hooft and W. J. Wadman Ca2+ Ions Block and Permeate Serotonin 5-HT3 Receptor Channels in Rat Hippocampal Interneurons J Neurophysiol, April 1, 2003; 89(4): 1864 - 1869. [Abstract] [Full Text] [PDF]