| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Neuroscience Center, Louisiana State University Health Science Center, New Orleans, LA 70112, USA
In the CA1 region of the hippocampus, LTP is thought to be initiated by a transient activation of NMDA receptors and is expressed as a persistent increase in synaptic transmission through AMPA receptors. To investigate the postsynaptic modifications of AMPA receptors involved in this enhanced synaptic transmission, the channel density and single-channel properties of extrasynaptic AMPA receptors located in synaptically active dendritic regions were examined following the induction of LTP. Following tetanic stimulation an outside-out patch was excised from the apical dendrite near the point of stimulation and saturating concentrations of glutamate were rapidly applied to the patch. AMPA current amplitude and duration were increased significantly in patches pulled from dendrites that expressed LTP. Non-stationary fluctuation analysis of AMPA currents indicated that AMPA channel number was nearly twofold larger than in controls, while single channel conductance and maximum open-probability were unchanged. Furthermore, while subtle changes in AMPA channel kinetics could also be observed, we did not find any evidence that receptor affinity or rectification properties were altered by LTP induction. Very similar results were found when CaMK-II activity was increased through the intracellular application of Ca/CaM. Together, we interpret our data to indicate that the stimuli used here produce an increased delivery of AMPA receptors to synaptically active regions of the apical dendrite without inducing any significant changes in their basic biophysical properties and that such delivery is a key element in this form of synaptic plasticity.
(Received 24 March 2004;
accepted after revision 29 June 2004;
first published online 2 July 2004)
Corresponding author B. K. Andrásfalvy: Neuroscience Center, Louisiana State University Health Science Center, New Orleans, LA 70112, USA. Email: andrasfalvy{at}koki.hu
This article has been cited by other articles:
|
|
 |
|
  Y. Yang, X.-b. Wang, M. Frerking, and Q. Zhou Delivery of AMPA receptors to perisynaptic sites precedes the full expression of long-term potentiation PNAS, August 12, 2008; 105(32): 11388 - 11393. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Pooler and R. A. J. McIlhinney Lateral Diffusion of the GABAB Receptor Is Regulated by the GABAB2 C Terminus J. Biol. Chem., August 31, 2007; 282(35): 25349 - 25356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gasparini, A. Losonczy, X. Chen, D. Johnston, and J. C. Magee Associative pairing enhances action potential back-propagation in radial oblique branches of CA1 pyramidal neurons J. Physiol., May 1, 2007; 580(3): 787 - 800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lisman and S. Raghavachari A Unified Model of the Presynaptic and Postsynaptic Changes During LTP at CA1 Synapses Sci. Signal., October 10, 2006; 2006(356): re11 - re11. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-l. Zhang, Z.-y. Zhou, J. Winterer, W. Muller, and P. K. Stanton NMDA-Dependent, But Not Group I Metabotropic Glutamate Receptor-Dependent, Long-Term Depression at Schaffer Collateral-CA1 Synapses Is Associated with Long-Term Reduction of Release from the Rapidly Recycling Presynaptic Vesicle Pool J. Neurosci., October 4, 2006; 26(40): 10270 - 10280. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. R. Holmes and L. M. Grover Quantifying the Magnitude of Changes in Synaptic Level Parameters With Long-Term Potentiation J Neurophysiol, September 1, 2006; 96(3): 1478 - 1491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. McDermott, M. N. Hardy, N. G. Bazan, and J. C. Magee Sleep deprivation-induced alterations in excitatory synaptic transmission in the CA1 region of the rat hippocampus J. Physiol., February 1, 2006; 570(3): 553 - 565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Oh, V. A. Derkach, E. S. Guire, and T. R. Soderling Extrasynaptic Membrane Trafficking Regulated by GluR1 Serine 845 Phosphorylation Primes AMPA Receptors for Long-term Potentiation J. Biol. Chem., January 13, 2006; 281(2): 752 - 758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. L. Bloodgood and B. L. Sabatini Neuronal Activity Regulates Diffusion Across the Neck of Dendritic Spines Science, November 4, 2005; 310(5749): 866 - 869. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Palmer, L. Cotton, and J. M. Henley The Molecular Pharmacology and Cell Biology of {alpha}-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Receptors Pharmacol. Rev., June 1, 2005; 57(2): 253 - 277. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
