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Neuroscience |
1 Department of Neurology and Neurological Sciences, Stanford University Medical Center, Stanford, CA 94305, USA
Synchrony within the thalamocortical system is regulated in part by intranuclear synaptic inhibition within the reticular nucleus (RTN). Inhibitory postsynaptic currents (IPSCs) in RTN neurons are largely characterized by slow decay kinetics that result in powerful and prolonged suppression of spikes. Here we show that some individual RTN neurons are characterized by highly variable mixtures of fast, slow and mixed IPSCs. Heterogeneity arose largely through differences in the contribution of an initial decay component (
D
10 ms) which was insensitive to loreclezole, suggesting involvement of the GABAA receptor ß1 subunit. Single-cell RT-PCR revealed the presence of ß1 subunit mRNA only in those neurons whose IPSCs were dominated by a rapid and prominent initial decay phase. These data show that brief, ß1-dependent, loreclezole-insensitive IPSCs are present in a subpopulation of RTN neurons, and suggest that striking differences in IPSC heterogeneity within single neurons can result from of the presence or absence of a single GABAA receptor subunit.
(Received 31 January 2006;
accepted after revision 6 February 2006;
first published online 9 February 2006)
Corresponding author J. R. Huguenard: Department of Neurology and Neurological Sciences, Room M016, Stanford University, Stanford University Medical Center, Stanford, CA 94305-5300, USA. Email: john.huguenard{at}stanford.edu
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