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SKELETAL MUSCLE AND EXERCISE |
1 Département de kinésiologie, Université de Montréal, C.P. 6128, Succursale Centre-Ville, Montréal, Québec, Canada H3C 3J7
2 HLHP Research Institute and Spinal Cord Research Center, Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
3 Laboratoire de Bioénergétique Fondamentale et Appliquée, Université Joseph Fourier, F-38041 Grenoble-cedex 09, France
Loss of neural input to skeletal muscle fibres induces atrophy and degeneration with evidence of mitochondria-mediated cell death. However, the effect of denervation on the permeability transition pore (PTP), a mitochondrial protein complex implicated in cell death, is uncertain. In the present study, the impact of 21 days of denervation on the sensitivity of the PTP to Ca2+-induced opening was studied in isolated muscle mitochondria. Muscle denervation increased the sensitivity to Ca2+-induced opening of the PTP, as indicated by a significant decrease in calcium retention capacity (CRC: 111 ± 12 versus 475 ± 33 nmol (mg protein)–1 for denervated and sham, respectively). This phenomenon was partly attributable to in vivo mitochondrial and whole muscle Ca2+ overload. Cyclosporin A, which inhibits PTP opening by binding to cyclophilin D (CypD), was significantly more potent in mitochondria from denervated muscle and restored CRC to the level observed in mitochondria from sham-operated muscles. In contrast, the CypD independent inhibitor trifluoperazine was equally effective at inhibiting PTP opening in sham and denervated animals and did not correct the difference in CRC between groups. This phenomenon was associated with a significant increase in the content of the PTP regulating protein CypD relative to several mitochondrial marker proteins. Together, these results indicate that Ca2+ overload in vivo and an altered expression of CypD could predispose mitochondria to permeability transition in denervated muscles.
(Received 14 March 2006;
accepted after revision 2 May 2006;
first published online 4 May 2006)
Corresponding author Y. Burelle: Université de Montréal, Department of kinesiology, P.O. Box 6128 Centre-Ville, Montreal, Quebec, Canada, H3C 3J7. Email: yan.burelle{at}umontreal.ca
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