HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 586/4/1059    most recent jphysiol.2007.146993v2 jphysiol.2007.146993v1 Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Delpy, A. Articles by Branchereau, P. Search for Related Content PubMed PubMed Citation Articles by Delpy, A. Articles by Branchereau, P. Related Collections Neuroscience

¹ Centre de Neuroscience Intégratives et Cognitives, Université Bordeaux and CNRS, UMR 5228, Avenue des Facultés, 33405 Talence cedex, France

Early in development, GABA and glycine exert excitatory action that turns to inhibition due to modification of the chloride equilibrium potential (E_Cl) controlled by the KCC2 and NKCC1 transporters. This switch is thought to be due to a late expression of KCC2 associated with a NKCC1 down-regulation. Here, we show in mouse embryonic spinal cord that both KCC2 and NKCC1 are expressed and functional early in development (E11.5–E13.5) when GABA_A receptor activation induces strong excitatory action. After E15.5, a switch occurs rendering GABA unable to provide excitation. At these subsequent stages, NKCC1 becomes both inactive and less abundant in motoneurons while KCC2 remains functional and hyperpolarizes E_Cl. In conclusion, in contrast to other systems, the cotransporters are concomitantly expressed early in the development of the mouse spinal cord. Moreover, whereas NKCC1 follows a classical functional extinction, KCC2 is highly expressed throughout both early and late embryonic life.

(Received 8 October 2007; accepted after revision 13 December 2007; first published online 20 December 2007)
Corresponding author P. Branchereau: Centre de Neuroscience Intégratives et Cognitives, Université Bordeaux et Centre National de la Recherche Scientifique Unité Mixte de Recherche 5228, Avenue des Facultés, 33405 Talence, France. Email: p.branchereau{at}cnic.u-bordeaux1.fr

This article has been cited by other articles:

				K. Szokol, J. C. Glover, and M.-C. Perreault Differential origin of reticulospinal drive to motoneurons innervating trunk and hindlimb muscles in the mouse revealed by optical recording J. Physiol., November 1, 2008; 586(21): 5259 - 5276. [Abstract] [Full Text] [PDF]

				Z. Wang, L. Li, M. Goulding, and E. Frank Early Postnatal Development of Reciprocal Ia Inhibition in the Murine Spinal Cord J Neurophysiol, July 1, 2008; 100(1): 185 - 196. [Abstract] [Full Text] [PDF]