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First published online on November 7, 2003.
 Copyright © 2003 by The Physiological Society
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Received July 21, 2003
Revised August 14, 2003
Accepted after revision November 3, 2003

Influence of age and resistance exercise on skeletal muscle proteolysis: A microdialysis approach

Todd Trappe1*, Rick Williams1, John Carrithers1, Ulrika Raue1, Birgitte Esmarck2, Michael Kjaer2, and Robert Hickner3

1 University of Arkansas for Medical Sciences
2 Copenhagen University Hospital at Bispebjerg
3 East Carolina University

* To whom correspondence should be addressed. E-mail: trappetodda{at}uams.edu.

We combined the interstitial sampling method of microdialysis with the natural tracer qualities (i.e., non-recyclable) of the amino acid 3-methylhistidine (3MH) to uniquely study in-vivo degradation of the two most abundant skeletal muscle proteins, myosin and actin. Interstitial 3MH concentration was measured before and for 24 h following a single bout of resistance exercise in eight young (27 ±2 y) and eight old (75 ±4 y) men. The exercise bout consisted of four exercises (3 sets of 8 reps @ 80% 1 RM per exercise) emphasizing the quadriceps. Interstitial 3MH concentration was calculated using the internal reference method from microdialysate samples that were obtained from two microdialysis probes placed in the vastus lateralis. Resting interstitial 3MH concentration was 44% higher (p<0.05) in the old (6.16 ±0.56 nmoloml-1) as compared with the young (4.28 ±0.27 nmoloml-1). Interstitial 3MH was not different (p>0.05) from preexercise at any time point within the 24 hours following exercise in both the young and old. Leg arteriovenous exchange measurements in a separate group of young subjects also showed no increase in 3MH release during the four hours following a resistance exercise bout compared with a non-exercised control leg (control leg: -28 ±6, exercise leg: - 28 ±11 nmolomin-1). These results suggest that myosin and actin proteolysis are not increased in the first 24 hours following a standard bout of resistance exercise, and this response is not altered with aging. The higher interstitial 3MH concentration in the old suggests an increased proteolysis of the two main contractile proteins in the rested and fasted state, which is consistent with a decrease in muscle mass with aging. Microdialysis is an appropriate methodology for use in aging individuals and is compatible with high- intensity resistance exercise.


Key words: Ageing • Protein metabolism • Skeletal muscle




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