| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Received September 25, 2003
Revised October 23, 2003
Accepted after revision October 23, 2003
1 Dept. Pharmcology & Toxicology, Medical Faculty TU Dresden
2 Medical Clinics I, Medical Faculty TU Dresden
* To whom correspondence should be addressed. E-mail: ravens{at}rcs.urz.tu-dresden.de.
Human mesenchymal stem cells (hMSC) have gained
considerable interest due to their potential use for
cell replacement therapy and tissue engineering. One
strategy is to in vitro differentiate these bone marrow
stem cells into cardiomyocytes prior to implantation. In
this context ion channels can be important functional
markers of cardiac differentiation. At present there is
little information about the electrophysiological
behaviour of the undifferentiated hMSC. We therefore
investigated mRNA expression of 26 ion channel subunits
using semi-quantitative RT-PCR and recorded
transmembrane ion currents with the whole-cell voltage
clamp technique. Bone marrow hMSC were obtained from
healthy donors. The cells revealed a distinct pattern of
ion channel mRNA with high expression levels for some
channel subunits (e.g. Kv4.2, Kv4.3, MaxiK, HCN2, and
1C of the L-type calcium channel). Outward
currents were recorded in almost all cells. The most
abundant outward current rapidly activated at potentials
positive to +20 mV. This current was identified as a
large-conductance voltage- and Ca2+-activated
K+ current, conducted by MaxiK channels, due
to its high sensitivity to tetraethylammonium
(IC50 = 340 µM) and its inhibition by
100 nM
iberiotoxin. A large fraction of cells also demonstrated
a more slowly activating current at potentials positive
to -30 mV. This current was selectively inhibited by
clofilium (IC50 = 0.8 µM).
Ba2+ inward currents, stimulated by
1 µM BayK 8644 were found in a few cells, indicating
expression of functional L-type Ca2+
channels. Other inward currents like sodium currents or
inward rectifier currents were absent. We conclude that
undifferentiated hMSC express a distinct pattern of ion
channel mRNA and functional ion channels that might
contribute to physiological cell function.
This article has been cited by other articles:
|
|
 |
|
  F. Sbrana, C. Sassoli, E. Meacci, D. Nosi, R. Squecco, F. Paternostro, B. Tiribilli, S. Zecchi-Orlandini, F. Francini, and L. Formigli Role for stress fiber contraction in surface tension development and stretch-activated channel regulation in C2C12 myoblasts Am J Physiol Cell Physiol, July 1, 2008; 295(1): C160 - C172. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Pedrotty, R. Y. Klinger, N. Badie, S. Hinds, A. Kardashian, and N. Bursac Structural coupling of cardiomyocytes and noncardiomyocytes: quantitative comparisons using a novel micropatterned cell pair assay Am J Physiol Heart Circ Physiol, July 1, 2008; 295(1): H390 - H400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Bai, J. Ma, Z. Pan, Y.-H. Song, S. Freyberg, Y. Yan, D. Vykoukal, and E. Alt Electrophysiological properties of human adipose tissue-derived stem cells Am J Physiol Cell Physiol, November 1, 2007; 293(5): C1539 - C1550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Tao, C.-P. Lau, H.-F. Tse, and G.-R. Li Functional ion channels in mouse bone marrow mesenchymal stem cells Am J Physiol Cell Physiol, November 1, 2007; 293(5): C1561 - C1567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Miragoli, N. Salvarani, and S. Rohr Myofibroblasts Induce Ectopic Activity in Cardiac Tissue Circ. Res., October 12, 2007; 101(8): 755 - 758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Park, K. H. Jung, S. H. Kim, K. S. Kim, M. R. Choi, Y. Kim, and Y. G. Chai Functional Expression of Ion Channels in Mesenchymal Stem Cells Derived from Umbilical Cord Vein Stem Cells, August 1, 2007; 25(8): 2044 - 2052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Coppi, A. M. Pugliese, S. Urbani, A. Melani, E. Cerbai, B. Mazzanti, A. Bosi, R. Saccardi, and F. Pedata ATP Modulates Cell Proliferation and Elicits Two Different Electrophysiological Responses in Human Mesenchymal Stem Cells Stem Cells, July 1, 2007; 25(7): 1840 - 1849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Schweizer, U. Krause, R. Becker, A. Seckinger, A. Bauer, C. Hardt, V. Eckstein, A. D. Ho, M. Koenen, H. A. Katus, et al. Atrial-Radiofrequency Catheter Ablation Mediated Targeting of Mesenchymal Stromal Cells Stem Cells, June 1, 2007; 25(6): 1546 - 1551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Pijnappels, M. J. Schalij, J. van Tuyn, D. L. Ypey, A. A.F. de Vries, E. E. van der Wall, A. van der Laarse, and D. E. Atsma Progressive increase in conduction velocity across human mesenchymal stem cells is mediated by enhanced electrical coupling Cardiovasc Res, November 1, 2006; 72(2): 282 - 291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-R. Li, X.-L. Deng, H. Sun, S. S.M. Chung, H.-F. Tse, and C.-P. Lau Ion Channels in Mesenchymal Stem Cells from Rat Bone Marrow Stem Cells, June 1, 2006; 24(6): 1519 - 1528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Chang, L. Tung, R. B. Sekar, C. Y. Chang, J. Cysyk, P. Dong, E. Marban, and M. R. Abraham Proarrhythmic Potential of Mesenchymal Stem Cell Transplantation Revealed in an In Vitro Coculture Model Circulation, April 18, 2006; 113(15): 1832 - 1841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Wang, T. Xue, S.-Y. Tsang, R. Van Huizen, C. W. Wong, K. W. Lai, Z. Ye, L. Cheng, K. W. Au, J. Zhang, et al. Electrophysiological Properties of Pluripotent Human and Mouse Embryonic Stem Cells Stem Cells, October 1, 2005; 23(10): 1526 - 1534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Berchtold, K.-S. Chen, S. Miyamoto, and M. N. Gould Perillyl Alcohol Inhibits a Calcium-Dependent Constitutive Nuclear Factor-{kappa}B Pathway Cancer Res., September 15, 2005; 65(18): 8558 - 8566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-R. Li, H. Sun, X. Deng, and C.-P. Lau Characterization of Ionic Currents in Human Mesenchymal Stem Cells from Bone Marrow Stem Cells, March 1, 2005; 23(3): 371 - 382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Boheler Functional markers and the 'homogeneity' of human mesenchymal stem cells J. Physiol., February 1, 2004; 554(3): 592 - 592. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
