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Received November 17, 2003
Revised January 8, 2004
Accepted after revision March 8, 2004
1 McMaster University
2 Brock University
3 Deakin University
4 McMaster University Health Center
* To whom correspondence should be addressed. E-mail: leblanpj{at}mcmaster.ca.
This study examined the effects of short and long-term
aerobic training on the stable up regulation of pyruvate
dehydrogenase (PDH) and PDH kinase (PDK) in human
skeletal muscle. We hypothesized that 8 weeks, but not
1 week, of aerobic training would increase total PDH
(PDHt) and PDK activities compared to pre-training, and
this would be detectable at the level of gene
transcription (mRNA) and/or gene translation (protein).
Resting muscle biopsies were taken before and after 1
and 8 weeks of aerobic cycle exercise training. PDHt
and PDK activities, and their respective protein and
mRNA expression, did not differ after 1 week of aerobic
training. PDHt activity increased 15% after 8 weeks and
this may be partially due to a 1.3 fold increase in
PDHE1
protein expression. PDK activity
approximately doubled after 8 weeks of aerobic training
and this was attributed to a 1.3 fold increase in PDK2
isoform protein expression. Similar to 1 week, no
changes were observed at the mRNA level after 8 weeks of
training. These findings suggest that aerobically
trained human skeletal muscle has an increased maximal
capacity to utilize carbohydrates, evident by increased
PDHt, but increased metabolic control sensitivity to
pyruvate through increased contribution of PDK2 to total
PDK activity.
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