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First published online on April 23, 2004.
 Copyright © 2004 by The Physiological Society
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Received December 16, 2003
Revised February 2, 2004
Accepted after revision April 22, 2004

Synaptic interactions between pyramidal cells and interneuron subtypes during seizure-like activity in the rat hippocampus

Yoko Fujiwara-Tsukamoto1, Yoshikazu Isomura1*, Katsuyuki Kaneda1, and Masahiko Takada1

1 Tokyo Metropolitan Institute for Neuroscience

* To whom correspondence should be addressed. E-mail: isomura{at}tmin.ac.jp.

We have recently reported that excitatory GABAergic and glutamatergic mechanisms may be involved in the generation of seizure-like (ictal) rhythmic synchronization (afterdischarge), induced by a strong synaptic stimulation, of the CA1 pyramidal cells in the mature rat hippocampus in vitro. To clarify the network mechanism of this neuronal synchronization, dual whole-cell patch-clamp recordings of the afterdischarge responses were performed simultaneously from a variety of interneurons and their neighboring pyramidal cells in hippocampal CA1-isolated slice preparations. According to morphological and electrophysiological criteria, the recorded interneurons were then classified into distinct subtypes. The non-fast-spiking interneurons located in the strata lacunosum-moleculare and radiatum hardly discharged during the afterdischarge, whereas most of the fast-spiking and non-fast-spiking interneurons in the strata oriens and pyramidale, including the basket, chandelier, and bistratified cells, exhibited prominent firings that were precisely synchronous with oscillatory responses in the pyramidal cells. Field potential recordings showed that excitatory synaptic transmissions might take place primarily in the strata oriens and pyramidale during the afterdischarge. Restricted lesions in the strata oriens and pyramidale, but not in the other layers, resulted in the complete desynchronization of afterdischarge activity, and also, local application of glutamate receptor antagonists to these layers greatly blocked the expression of afterdischarge. The present findings indicate that the neuronal synchronization of epileptic afterdischarge may be accomplished in a 'positive feedback circuit' formed by the excitatory GABAergic interneurons and the glutamatergic pyramidal cells within the strata oriens and/or pyramidale of the hippocampal CA1 region.


Key words: Hippocampus • Interneurone • Oscillation




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