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Received February 18, 2004
Revised March 11, 2004
Accepted after revision June 17, 2004
1 University of Nevada School of Medicine
2 University of Nevada
3 Australian National University
* To whom correspondence should be addressed. E-mail: david.hirst{at}anu.edu.au.
Intracellular recordings were made from isolated bundles of the circular muscle layer of mouse and guinea-pig gastric fundus. These preparations displayed an on going discharge of membrane noise (unitary potentials), similar to that recorded from similar preparations made from the circular layer of the antrum. Bundles of muscle from the fundus of W/WV mice, which lack intramuscular interstitial cells of Cajal, ICCIM, lacked the discharge of membrane noise observed in wild-type tissues. When the membrane potential was changed by passing depolarizing or hyperpolarizing current pulses, the discharge of membrane noise was little changed. The membrane noise was unaffected by adding chloride channel blockers however, agents which buffered the internal concentration of calcium ions reduced the discharge of membrane noise. Treatment of tissues with CCCP, which interfere with the uptake of calcium ions by mitochondria, also reduced the membrane noise and caused membrane hyperpolarization. Similar observations were made on bundles of tissue isolated from the circular layer of the guinea-pig antrum. Together the observations indicate that membrane noise is generated by a pathway located in ICCIM. This properties of the pathway appear to vary dramatically within a given organ. The lack of voltage sensitivity of the discharge of membrane noise in the fundus provides a possible explanation for the lack of rhythmic electrical activity in this region of the stomach.
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