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First published online on June 4, 2004.
 Copyright © 2004 by The Physiological Society
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jphysiol.2004.066845v1
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Received April 21, 2004
Revised May 21, 2004
Accepted after revision May 31, 2004

P2X receptor subtype specific modulation of excitatory and inhibitory synaptic inputs in the rat brainstem

Tomokazu Watano1, Jennifer A Calvert1, Catherine Vial1, Ian D Forsythe1, and Richard J Evans1*

1 University of Leicester

* To whom correspondence should be addressed. E-mail: rje6{at}le.ac.uk.

The role of P2 receptors in synaptic transmission to the rat medial nucleus of the trapezoid body (MNTB) was studied in an in vitro brain slice preparation. Whole-cell patch recordings were made and spontaneous synaptic responses studied under voltage clamp during application of P2X agonists. ATPgS (100 microM) had no effect on holding current, but facilitated spontaneous excitatory postsynaptic current (sEPSC) frequency in 41 % of recordings and facilitated spontaneous inhibitory postsynaptic currents (sIPSCs) in 20 % of recordings. These were blocked by the P2 receptor antagonists suramin (100 microM). a,b meATP also facilitated sEPSC and sIPSC frequency, while l-b,g-meATP facilitated only sIPSCs. The sEPSC facilitation by ATPgS was blocked by TTX (but did not block facilitation of sIPSCs). sEPSC facilitation was blocked by PPADS (30 microM), the selective P2X3 receptor antagonist A-317491 (3 microM) suggesting that modulation of sEPSCs involves P2X3 receptor subunits. a,b meATP facilitated sIPSCs were also recorded in wild type mouse MNTB neurons, but were absent in the MNTB from P2X1 receptor deficient mice demonstrating a functional role for P2X1 receptors in the CNS.


Key words: Adenosine triphosphate • Brainstem • P2 purinoceptor




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