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First published online on October 7, 2004.
 Copyright © 2004 by The Physiological Society
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Received July 26, 2004
Revised August 31, 2004
Accepted after revision October 5, 2004

In vivo responses of mouse superficial dorsal horn neurones to both current injection and peripheral cutaneous stimulation

Brett A Graham1, Alan M Brichta1, and Robert J Callister1*

1 University of Newcastle

* To whom correspondence should be addressed. E-mail: robert.callister{at}newcastle.edu.au.

In the superficial dorsal horn (SDH) processing of noxious and innocuous stimuli is critically dependent on the input-output relationship of its component neurones. Such relationships are routinely examined by assessing neuronal responses to somatic current injection or activation of synaptic inputs. A more complete understanding of input-output relationships would be achieved by comparing, in the same neurone, how the two forms of activation contribute to neuronal output. Therefore, we examined how SDH neurones transform depolarising current injections and synaptic excitation via peripheral cutaneous stimuli (brush and pinch of the hindpaw) into trains of action potentials, in an in vivo preparation of the adult mouse spinal cord. Under whole-cell current clamp recording conditions four action potential discharge patterns were observed during depolarising current injection: tonic firing neurones (21/93) discharged spikes throughout the step; initial bursting neurones (35/93) discharged several spikes at step onset; single spiking neurones (16/93) discharged one or two spikes at step onset; and delayed firing neurones (21/93) discharged spikes delayed from the step onset. Four characteristic profiles were observed in response to application of noxious (pinch) and innocuous (brush) cutaneous stimuli: nociceptive neurones (20/37) responded maximally to pinch stimulation; light touch neurones (9/37) responded maximally to brush stimulation, subthreshold neurones (4/37) exhibited depolarising responses without firing action potentials; and hyperpolarising neurones (4/37) exhibited a sustained pinch-induced hyperpolarisation. Comparisons of current-evoked discharge patterns with peripherally-evoked responses indicate SDH neurones expressing each of the four discharge patterns could receive, and therefore participate in the processing of information concerning, either noxious or innocuous stimuli. These data suggest that a neurone's response to current injection does not necessarily help identify or predict how the same neurone will respond to physiologically- or functionally-relevant stimuli.


Key words: Electrophysiology • Pain • Spinal cord




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