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Received March 4, 2005
Revised March 18, 2005
Accepted after revision March 24, 2005
1 Institute of Physiology, School of Medicine, National Yang-Ming University
2 Department of Physiology, Taipei Medical University
3 Institute of Pharmacology, School of Medicine, National Yang-Ming University
* To whom correspondence should be addressed. E-mail: yrkou{at}ym.edu.tw.
The mechanisms of sensory transduction of pulmonary reactive oxygen species (ROS) by capsaicin-sensitive vagal lung afferent fibres are unclear. To investigate the role of transient receptor potential vanilloid 1 (TRPV1) receptors and P2X purinoceptors in this sensory transduction, we recorded activity (FA) from 132 fibres of this type in 132 anaesthetized and ventilated rats. Airway challenge of aerosolized H2O2 (0, 0.2 and 0.4 %) produced a concentration-dependant fibre stimulation. The fibre responses to 0.4 % H2O2 were attenuated by dimethylthiourea [a hydroxyl radical (·OH) scavenger; 
FA, -55 ± 9 %] or deferoxamine (an iron-chelator preventing formation of ·OH; FA, -59 ± 9 %), were prevented by catalase (an enzyme catalyzing H2O2; FA, -96 ± 3 %) and were unaffected by the vehicle for dimethylthiourea, iron-saturated deferoxamine or heat-inactivated catalase. The fibre responses to 0.4 % H2O2 were attenuated by capsazepine (a TRPV1 receptor antagonist; FA, -39 ± 9 %) or iso-pyridoxalphosphate-6-azophenyl-2',5'-disulphonate (iso-PPADS, a P2X receptor antagonist; FA, -51 ± 9 %), were further reduced by capsazepine and iso-PPADS in combination (FA, -70 ± 13 %), and were unaltered by their vehicles. The fibre responses to cigarette smoke (20 ml; an irritant generating ROS) were attenuated by dimethylthiourea (FA, -61 ± 9 %) or capsazepine and iso-PPADS in combination (FA, -67 ± 9 %). These results suggest that both the TRPV1 and P2X receptors mediate the sensory transduction of ROS, especially H2O2 and ·OH, by capsaicin-sensitive vagal lung afferent fibres.
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