J Physiol Society Membership
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Physiology in Press

First published online on April 28, 2005.
 Copyright © 2005 by The Physiological Society
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
566/1/205    most recent
jphysiol.2005.087577v1
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, M.-G.
Right arrow Articles by Undem, B. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, M.-G.
Right arrow Articles by Undem, B. J.

Received March 28, 2005
Revised April 22, 2005
Accepted after revision April 22, 2005

"Role of Chloride Channels in Bradykinin-Induced Guinea Pig Airway Vagal C-fiber Activation"

Min-Goo Lee1, Donald MacGlashan Jr.1, and Bradley J. Undem1*

1 Johns Hopkins University

* To whom correspondence should be addressed. E-mail: bundem{at}jhmi.edu.

We tested the hypothesis that an ionic current carried by chloride ions contributes to bradykinin (BK)-induced membrane depolarization and activation of vagal afferent C-fibers. In an ex vivo innervated trachea/bronchus preparation, BK (1 µM) consistently produced action potential discharge in vagal afferent C-fibers with receptive fields in the trachea or main stem bronchus. The calcium-activated chloride channel (CLCA) inhibitor, niflumic acid (NFA, 100 µM), significantly reduced BK-induced action potential discharge to 21 ± 7% of the control BK response. NFA did not inhibit capsaicin-induced or citric acid-induced action potential discharge in tracheal C-fibers. The inhibitory effect of NFA was mimicked by another CLCA inhibitor, 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB, 100 µM). NFA also inhibited the BK-induced inward current in gramicidin-perforated whole cell patch clamp recordings of capsaicin-sensitive jugular ganglion neurons retrogradely labeled from the airways. NFA did not inhibit the BK-induced increase in intracellular free calcium. The TRPV1 inhibitor, iodo-resiniferatoxin (1 µM), also partially inhibited BK-induced action potential discharge, and the combination of iodo-resiniferatoxin and NFA virtually abolished the BK-induced action potential discharge. We concluded that in vagal afferent C-fibers, BK evokes membrane depolarization and action potential discharge through the additive effects of TRPV1 and chloride channel activation.


Key words: Bradykinin • C fibre • Chloride channel




This article has been cited by other articles:


Home page
 J. Physiol.Home page
K. Kwong, M. J. Carr, A. Gibbard, T. J. Savage, K. Singh, J. Jing, S. Meeker, and B. J. Undem
Voltage-gated sodium channels in nociceptive versus non-nociceptive nodose vagal sensory neurons innervating guinea pig lungs
J. Physiol., March 1, 2008; 586(5): 1321 - 1336.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. Qin, J. P. Farber, K. E. Miller, and R. D. Foreman
Responses of thoracic spinal neurons to activation and desensitization of cardiac TRPV1-containing afferents in rats
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2006; 291(6): R1700 - R1707.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. Taylor-Clark and B. J. Undem
Transduction mechanisms in airway sensory nerves
J Appl Physiol, September 1, 2006; 101(3): 950 - 959.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
B. Chuaychoo, M.-G. Lee, M. Kollarik, R. Pullmann Jr, and B. J. Undem
Evidence for both adenosine A1 and A2A receptors activating single vagal sensory C-fibres in guinea pig lungs
J. Physiol., September 1, 2006; 575(2): 481 - 490.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. B. Mazzone and A. E. McGovern
Na+-K+-2Cl- cotransporters and Cl- channels regulate citric acid cough in guinea pigs
J Appl Physiol, August 1, 2006; 101(2): 635 - 643.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
B. J. Canning, D. G. Farmer, and N. Mori
Mechanistic studies of acid-evoked coughing in anesthetized guinea pigs
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2006; 291(2): R454 - R463.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
H. Kaneko, F. Mohrlen, and S. Frings
Calmodulin Contributes to Gating Control in Olfactory Calcium-activated Chloride Channels
J. Gen. Physiol., May 30, 2006; 127(6): 737 - 748.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M.-G. Lee, B. J. Undem, C. Brown, and M. J. Carr
Effect of Nociceptin in Acid-evoked Cough and Airway Sensory Nerve Activation in Guinea Pigs
Am. J. Respir. Crit. Care Med., February 1, 2006; 173(3): 271 - 275.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C.H. Yeung, J.P. Barfield, and T.G. Cooper
Chloride Channels in Physiological Volume Regulation of Human Spermatozoa
Biol Reprod, November 1, 2005; 73(5): 1057 - 1063.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2005 The Physiological Society.