| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Received March 29, 2006
Revised April 24, 2006
Accepted after revision June 13, 2006
1 Karolinska Institutet
2 University of Kentucky Medical Center
* To whom correspondence should be addressed. E-mail: abram.katz{at}fyfa.ki.se.
Exercise increases glucose transport via a pathway that is poorly understood. We investigated the role of endogenously produced reactive oxygen species (ROS) in contraction-mediated glucose transport. Repeated contractions increased 2-deoxyglucose (2-DG) uptake ~3-fold in isolated, mouse extensor digitorum longus (fast-twitch) muscle. N-acetylcysteine (NAC), a nonspecific antioxidant, inhibited contraction-mediated 2-DG uptake by ~50% (P<0.05 vs control), but did not significantly affect basal 2-DG uptake or the uptake induced by insulin, hypoxia or 5-aminoimidazole-4-carboxamide-1-â-D-ribofuranoside (AICAR, mimics AMP-mediated activation of AMP-activated protein kinase, AMPK). Ebselen, a glutathione peroxidase mimetic, also inhibited contraction-mediated 2-DG uptake (almost 60%, P<0.001 vs. control). Muscles from mice over-expressing Mn-superoxide dismutase, which catalyzes H2O2 production from superoxide anions, exhibited an ~25% higher rate of contraction-mediated 2-DG uptake vs. muscles from wild type controls (P<0.05). Exogenous H2O2 induced oxidative stress, as judged by an increase in the GSSG/[GSH+GSSG] ratio to 2.5-fold of control and this increase was substantially blocked by NAC. Similarly, NAC significantly attenuated contraction-mediated oxidative stress as judged by measurements of glutathione status and the intracellular ROS level with the fluorescent indicator 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein (P<0.05). Last, contraction increased AMPK activity and phosphorylation ~10-fold and NAC blocked ~50% of these changes. These data indicate that endogenously produced ROS, possibly H2O2 or its derivatives, play an important role in contraction-mediated activation of glucose transport in fast-twitch muscle.
This article has been cited by other articles:
|
|
 |
|
  Y. Higaki, T. Mikami, N. Fujii, M. F. Hirshman, K. Koyama, T. Seino, K. Tanaka, and L. J. Goodyear Oxidative stress stimulates skeletal muscle glucose uptake through a phosphatidylinositol 3-kinase-dependent pathway Am J Physiol Endocrinol Metab, May 1, 2008; 294(5): E889 - E897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. P. Whitehead, C. Pham, O. L. Gervasio, and D. G. Allen N-Acetylcysteine ameliorates skeletal muscle pathophysiology in mdx mice J. Physiol., April 1, 2008; 586(7): 2003 - 2014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Bruton, N. Place, T. Yamada, J. P. Silva, F. H. Andrade, A. J. Dahlstedt, S.-J. Zhang, A. Katz, N.-G. Larsson, and H. Westerblad Reactive oxygen species and fatigue-induced prolonged low-frequency force depression in skeletal muscle fibres of rats, mice and SOD2 overexpressing mice J. Physiol., January 1, 2008; 586(1): 175 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Ross, G. D. Wadley, M. G. Clark, S. Rattigan, and G. K. McConell Local Nitric Oxide Synthase Inhibition Reduces Skeletal Muscle Glucose Uptake but Not Capillary Blood Flow During In Situ Muscle Contraction in Rats Diabetes, December 1, 2007; 56(12): 2885 - 2892. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-J. Zhang, M. E. Sandstrom, J. T. Lanner, A. Thorell, H. Westerblad, and A. Katz Activation of aconitase in mouse fast-twitch skeletal muscle during contraction-mediated oxidative stress Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1154 - C1159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. N. Edwards, W. A. Macdonald, C. van der Poel, and D. G. Stephenson O2bullet production at 37{degrees}C plays a critical role in depressing tetanic force of isolated rat and mouse skeletal muscle Am J Physiol Cell Physiol, August 1, 2007; 293(2): C650 - C660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. F. Kramer and L. J. Goodyear Exercise, MAPK, and NF-{kappa}B signaling in skeletal muscle J Appl Physiol, July 1, 2007; 103(1): 388 - 395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Clanton Hypoxia-induced reactive oxygen species formation in skeletal muscle J Appl Physiol, June 1, 2007; 102(6): 2379 - 2388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Jensen, A. J. Rose, S. B. Jorgensen, N. Brandt, P. Schjerling, J. F. P. Wojtaszewski, and E. A. Richter Possible CaMKK-dependent regulation of AMPK phosphorylation and glucose uptake at the onset of mild tetanic skeletal muscle contraction Am J Physiol Endocrinol Metab, May 1, 2007; 292(5): E1308 - E1317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pelletier and L. Coderre Ketone bodies alter dinitrophenol-induced glucose uptake through AMPK inhibition and oxidative stress generation in adult cardiomyocytes Am J Physiol Endocrinol Metab, May 1, 2007; 292(5): E1325 - E1332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Katz Modulation of glucose transport in skeletal muscle by reactive oxygen species J Appl Physiol, April 1, 2007; 102(4): 1671 - 1676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Sandstrom, S.-J. Zhang, H. Westerblad, and A. Katz Mechanical load plays little role in contraction-mediated glucose transport in mouse skeletal muscle J. Physiol., March 1, 2007; 579(2): 527 - 534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Freyssenet Energy sensing and regulation of gene expression in skeletal muscle J Appl Physiol, February 1, 2007; 102(2): 529 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. W. Balon Many pathways are called! Many may be chosen! J. Physiol., August 15, 2006; 575(1): 3 - 3. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
