In the nervous system, zinc can influence synaptic responses and at extreme concentrations contributes to epileptic and ischemic neuronal injury. Zinc can originate from synaptic vesicles, the extracellular space and from intracellular stores. In this study, we aimed to determine which of these zinc pools is responsible for the increased hippocampal excitability observed in zinc depleted/chelated animals. Also, we investigated the source of intracellularly accumulating zinc in vulnerable neurons. Our data indicate that membrane-permeable and membrane-impermeable zinc chelators had little or no effect on seizure activity in the CA3 region. Futhermore, extracellular zinc chelation could not prevent the accumulation of lethal concentrations of zinc in dying neurons following epileptic seizures. At the electron microscopic level, zinc-staining significantly increased at the presynaptic membrane of mossy fibre terminals in kainic acid treated animals. These data indicate that intracellular but not extracellular zinc chelators could influence neuronal excitability and seizure-induced zinc accumulation observed in the cytosol of vulnerable neurons.