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The cover illustration shows time lapsed microscopy showing that atropine reverses fluorescent labelling of tracheal smooth muscle by FM1-43, viewed intravitally in wholemounts of the guinea pig trachea. Alternate columns are taken from control and atropine treated tissues. Receptor binding assays, functional analyses and imaging studies such as the experiment depicted in this figure show that the styryl dyes FM1-43 and FM2-10 are competitive antagonists of human, guinea pig, rat and cat muscarinic receptors. See Mazzone et al. pp. 23–35.