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The cover image shows images of ECFP (left two columns) and EYFP (right two columns) emissions from Chinese hamster ovary cells expressing a membrane targeted tandem construct of ECFP and either EYFP (Rho-pYC; upper two rows) or EYFP tagged WT CaM (lower two rows) before (first and third columns) and after (second and fourth columns) selective photobleaching of EYFP. Experiments were conducted with either 2 mM Ca 2⁺ (first and third rows) or 10 mM EGTA (second and fourth rows) in the bath. From Bal et al. pp.2307–2320 .